DHX30 (NM_014966) Human Untagged Clone

SKU SC110281

DHX30 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DHX30
• Synonyms: DDX30; NEDMIAL; RETCOR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014966, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCAGCTTCTAGGGACCTATTAAAAGAGTTCCCACAGCCCAAAAATCTTCTCAACAGTGTGATTGGAA; GAGCCCTCGGCATCTCACATGCAAAAGACAAACTAGTCTACGTGCACACAAATGGACCGAAGAAAAAGAA; AGTCACACTGCACATAAAATGGCCCAAGAGCGTGGAGGTAGAAGGCTATGGCAGCAAGAAGATCGATGCT; GAGCGGCAGGCTGCAGCTGCAGCCTGCCAGCTGTTCAAGGGTTGGGGTCTGCTAGGTCCCCGGAATGAGT; TGTTTGACGCAGCCAAATACCGAGTGCTAGCTGATCGCTTTGGCTCCCCTGCCGACAGCTGGTGGCGTCC; GGAACCCACCATGCCCCCTACTTCCTGGCGGCAGCTGAATCCAGAGAGTATTCGACCAGGGGGACCTGGG; GGCCTATCCCGCTCTTTAGGCCGGGAAGAAGAGGAGGACGAGGAGGAAGAGCTAGAAGAAGGGACCATAG; ATGTTACCGACTTCTTGTCCATGACCCAGCAGGATTCCCACGCTCCACTCAGGGACTCAAGGGGGAGTTC; CTTTGAGATGACAGATGACGACAGTGCCATTAGGGCTCTGACCCAGTTTCCACTTCCCAAGAACCTTCTG; GCCAAGGTGATTCAGATTGCAACGTCATCCTCCACAGCTAAGAACCTCATGCAGTTCCATACTGTGGGCA; CCAAGACCAAGCTGTCTACACTCACCCTGCTCTGGCCCTGCCCCATGACCTTTGTTGCCAAAGGGCGCCG; CAAAGCAGAGGCTGAGAATAAGGCGGCAGCCTTGGCCTGCAAGAAACTGAAGAGCCTGGGCCTGGTGGAC; AGGAACAACGAACCGCTTACACACGCCATGTATAACCTGGCCTCTTTGCGTGAGCTGGGTGAGACCCAGC; GCCGACCATGCACCATCCAGGTGCCCGAGCCCATCCTCCGCAAGATAGAGACCTTCCTGAACCATTACCC; TGTGGAGAGTTCATGGATCGCCCCAGAACTCCGGCTGCAGAGTGATGACATCTTGCCCTTGGGCAAGGAC; TCAGGGCCTCTGAGTGACCCTATCACAGGCAAGCCCTATGTGCCCCTGTTGGAAGCAGAGGAGGTACGTC; TCAGCCAGAGTCTGCTAGAACTGTGGCGGCGGCGAGGGCCGGTCTGGCAGGAGGCCCCCCAGCTACCTGT; GGACCCACATCGGGACACCATCCTCAACGCCATTGAGCAGCACCCGGTGGTGGTCATCTCTGGGGACACG; GGCTGTGGGAAGACCACGCGCATCCCCCAGCTGTTGCTGGAGCGCTATGTGACCGAGGGCCGAGGTGCCC; GCTGCAATGTTATCATCACCCAACCTCGCCGCATCTCTGCTGTGTCTGTGGCACAGCGGGTCAGCCACGA; ACTGGGCCCCTCCCTGCGCCGGAATGTGGGCTTCCAGGTGCGGTTGGAAAGTAAGCCCCCATCCCGAGGC; GGGGCCCTGCTCTTCTGCACTGTGGGTATCCTGCTGCGTAAGCTGCAGAGCAACCCCAGCCTGGAGGGCG; TGAGCCACGTCATCGTGGATGAGGTGCATGAGCGGGACGTGAACACAGACTTTCTGCTGATCCTGCTCAA; GGGCCTGCAGCGGCTCAACCCGGCCCTGCGGCTGGTGCTCATGAGTGCCACAGGGGACAATGAGCGCTTC; TCCCGATACTTTGGTGGCTGCCCCGTCATCAAGGTGCCTGGCTTCATGTACCCAGTCAAGGAGCACTACC; TAGAGGACATCCTGGCCAAGTTGGGCAAGCACCAGTACCTGCACCGGCACCGGCACCATGAGTCTGAGGA; TGAATGCGCACTCGATTTGGACCTTGTGACTGATCTGGTTCTGCACATCGATGCTCGCGGGGAACCAGGT; GGGATCCTGTGCTTCCTGCCTGGGTGGCAGGAGATCAAAGGAGTGCAGCAGCGCCTCCAGGAGGCCCTGG; GCATGCACGAGAGCAAGTACCTCATCCTGCCAGTGCACTCCAACATCCCCATGATGGATCAGAAGGCCAT; ATTCCAGCAGCCTCCAGTTGGGGTGCGCAAGATTGTCTTGGCCACCAACATTGCTGAGACTTCCATCACA; ATCAATGACATCGTGCATGTGGTGGACAGTGGGCTGCACAAGGAAGAACGCTATGACCTGAAGACCAAGG; TGTCCTGCCTGGAGACAGTGTGGGTATCAAGAGCCAATGTGATCCAGCGCCGGGGCCGGGCGGGCCGCTG; CCAGTCCGGCTTTGCCTACCACTTGTTCCCTCGAAGCCGGCTGGAGAAAATGGTCCCTTTCCAAGTGCCA; GAGATCCTGCGCACACCTCTTGAGAACCTGGTGCTGCAAGCGAAAATCCACATGCCTGAGAAGACGGCGG; TGGAGTTCCTGTCCAAGGCTGTGGACAGTCCAAACATCAAGGCAGTGGACGAGGCTGTGATCTTGCTCCA; GGAGATCGGGGTGCTGGACCAGCGGGAGTACCTGACTACCCTGGGGCAGCGCCTGGCTCACATCTCCACC; GACCCCCGGTTGGCCAAGGCCATTGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTACTGGTGGTCG; TTTCCTGCCTCACCCGGGACCCCTTCAGCAGCAGCCTACAGAACCGGGCAGAGGTGGACAAGGTGAAAGC; ACTGTTGAGCCATGACAGCGGCAGTGACCACCTGGCCTTTGTGCGGGCTGTCGCCGGCTGGGAGGAGGTG; CTGCGTTGGCAGGACCGCAGCTCCCGGGAGAATTACCTGGAGGAAAACCTGCTGTACGCACCCAGCCTGC; GCTTCATCCACGGACTCATCAAGCAGTTCTCAGAGAACATTTATGAGGCCTTCCTGGTGGGGAAGCCCTC; GGACTGCACCCTGGCCTCCGCCCAGTGCAACGAGTACAGTGAGGAGGAGGAGCTGGTGAAGGGCGTGCTG; ATGGCCGGCCTCTACCCCAACCTCATCCAGGTGAGGCAGGGCAAGGTCACCCGGCAGGGGAAGTTCAAGC; CCAACAGCGTCACATATAGGACCAAATCAGGCAACATCCTGCTGCACAAGTCGACCATTAACAGGGAGGC; CACACGGTTACGGAGCCGATGGCTGACGTATTTCATGGCAGTCAAGTCCAATGGCAGCGTCTTCGTCCGG; GACTCCTCTCAGGTGCACCCGCTAGCTGTGCTGCTGCTGACCGACGGGGACGTGCACATCCGTGATGACG; GGCGCCGGGCCACCATCTCACTGAGCGACAGTGACCTGCTGCGGCTGGAGGGTGACTCGCGTACCGTGCG; GCTGCTGAAGGAGCTGCGGCGGGCCCTGGGCCGCATGGTGGAGCGGAGCCTGCGCAGCGAGCTGGCTGCA; CTTCCCCCCAGCGTACAGGAGGAGCACGGGCAGCTGCTTGCGCTACTGGCAGAGCTGCTGCGAGGACCCT; GTGGCAGCTTTGATGTGCGCAAGACAGCTGACGACTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014966 unedited; NGTTGGCGCATTTGTATACGATTCCTATAGGCGGCCCGCGAATTCGCACGAGGGCTCATG; CGCGGTGCACAGAGGCTTGTTTCACATCTGTAACAACAGGAGGAGGCCCAGCCTCGTGAT; GAGGAATAGCAAGGAGAGAATTCAGCTCCAGTTCAAAAGCCTACAAAATCTGAGACTGTC; ATTGCTTTTATAAGGATTCCAGCTTTCCCTCCTGGCCAGAAATGTTCAGCCTGGACTCAT; TCAGAAAAGATCGGGCCCAGCACAGGCAGCGTCAGTGCAAACTTCCCCCACCCCGCCTTC; CACCCATGTGTGTCAACCCTACCCCAGGAGGGACCATCTCTCGAGCTTCTAGGGACCTAT; TAAAAGAGTTCCCACAGCCCAAAAATCTTCTCAACAGTGTGATTGGAAGAGCCCTCGGCA; TCTCACATGCAAAAGACAAACTAGTCTACGTGCACACAAATGGACCGAAGAAAAAGAAAG; TCACACTGCACATAAAATGGCCCAAGAGCGTGGAGGTAGAAGGCTATGGCAGCAAGAAGA; TCGATGCTGAGCGGCAGGCTGCAGCTGCAGCCTGCCAGCTGTTCAAGGGTTGGGGTCTGC; TAGGTCCCCGGAATGAGTTGTTTGACGCAGCCAAATACCGAGTGCTAGCTGATCGCTTTG; GCTCCCCTGCCGACAGCTGGTGGCGTCCGGAACCCACCATGCCCCCTACTTCCTGGCGGC; AGCTGAATCCAGAGAGTATTCGACCAGGGNGGACCTGGGGGCCTATCCCGCTCTTTTAGG; CCGGAGAAAAGAGAGACGAGGAGGAAGAGCTAGAAGAAAGGACCATAGATGTTACCGACT; TCTGTCCATGACCCAGCAGGATTCCCACGCTCCACTCAGGGACTCAAGGGGNAGTTCCTT; TGAGAGACAGATGACGACAGTGCCATTTAGGCTCTGACCCAGTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014966 unedited; TGGCCGCGGCCGCAATCTATATCGAGTNCTTTTTTTTTTTTTTTTTTTTTTTCACACAAG; GGATAAATAGAACTTTATTTTAAATAAACATTGGCACTCTGTACACAGCCCCAGCAAAAG; CAGGGCTCATTCGTCAGCTGTCTTGCGCACATCAAAGCTGCCACAGGGTCCTCGCAGCAG; CTCTGCCATTAGCGCAAGCAGCTGCCCGTGCTCCTCCTGTACGCTGGGGGGAAGTGCAGC; CAGCTCGCTGCGCAGGCTCCGCTCCACCATGCGGCCCAGGGCCCGCCGAAGCTCCTTCAG; CAGCCGCACGGTACGCGAGTCACCCTCCAGCCGCAGCAGGTCACTGTCGCTCATTGAGAT; GGTGGCCCGGCGCCCGTCATCACGGATGTGCACGTCCCCGTCGGTCAGCAGCAGCACAGC; TAGCGGGGGCCCCTGATAGGAGTCCCGGACAAAGACGCTGCCATTGGACTTGACGGCCAT; GAAATACGCCAGCCCTCCGGCCCGGTATCCGTGGGGCCCCCCTGTAATGGGCCACTTGGG; CACCAGGAAGCGGCCGGGTCTGGGTCCCTTTGTGACCCTGTTGGGCTTTATCTTCCCCCG; CCCGGTTACTCGCTCCCCCTGCCTCGTTCCCGTTCGGGCTCACCCCCGCCCTCCCACCCC; TCATACTCGCCCCCGGCGCCCCGTCCCCTAGCCTCCATAGCTTCCCTCTCCCCTCCCTTG; CCCCCCCCCACCGCGACCCGCCCAGACCCGTGTGCTTTAGGTGCGCGCGTTTTCGCCCCT; TCCCCCCACCACTACGCGCTCCATGCCCCACCCACTGTCTATCGAGCTTCCTTTTGTCTC; CCCCCTTCCCTCCTCCAATCCCCTCGCGCCTTCCTGCGCCCGCTCGTTTCTCCTCCCGCC; CCATTTTTTCTTTTTGCTTTCCTCCTTACACTTTCGTCGCTCGTCTCTCTTGCTGTCGCA; TCGTCTTTTTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014966
• Insert Size: 3670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014966.2, NP_055781.2
• RefSeq Size: 3880 bp
• RefSeq ORF: 3468 bp
• Locus ID: 22907
• UniProt ID: Q7L2E3
• Cytogenetics: 3p21.31
• Domains: DSRM, HA2, helicase_C
• Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]; Transcript Variant: This variant (2) differs in its 5' UTR and uses an alternate translational start codon, compared to variant 1. The resulting isoform (2) has a distinct and shorter N-terminus, compared to isoform 1.