CAMKK2 (NM_172226) Human Untagged Clone

SKU SC110186

CAMKK2 (untagged)-Human calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), transcript variant 7

Product Data

• Type: Human Untagged Clone
• Target Symbol: CAMKK2
• Synonyms: CAMKK; CAMKKB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_172226 edited; ATGTCATCATGTGTCTCTAGCCAGCCCAGCAGCAACCGGGCCGCCCCCCAGGATGAGCTG; GGGGGCAGGGGCAGCAGCAGCAGCGAAAGCCAGAAGCCCTGTGAGGCCCTGCGGGGCCTC; TCATCCTTGAGCATCCACCTGGGCATGGAGTCCTTCATTGTGGTCACCGAGTGTGAGCCG; GGCTGTGCTGTGGACCTCGGCTTGGCGCGGGACCGGCCCCTGGAGGCCGATGGCCAAGAG; GTCCCCCTTGACACCTCCGGGTCCCAGGCCCGGCCCCACCTCTCCGGTCGCAAGCTGTCT; CTGCAAGAGCGGTCCCAGGGTGGGCTGGCAGCCGGTGGCAGCCTGGACATGAACGGACGC; TGCATCTGCCCGTCCCTGCCCTACTCACCCGTCAGCTCCCCGCAGTCCTCGCCTCGGCTG; CCCCGGCGGCCGACAGTGGAGTCTCACCACGTCTCCATCACGGGTATGCAGGACTGTGTG; CAGCTGAATCAGTATACCCTGAAGGATGAAATTGGAAAGGGCTCCTATGGTGTCGTCAAG; TTGGCCTACAATGAAAATGACAATACCTACTATGCAATGAAGGTGCTGTCCAAAAAGAAG; CTGATCCGGCAGGCCGGCTTTCCACGTCGCCCTCCACCCCGAGGCACCCGGCCAGCTCCT; GGAGGCTGCATCCAGCCCAGGGGCCCCATTGAGCAGGTGTACCAGGAAATTGCCATCCTC; AAGAAGCTGGACCACCCCAATGTGGTGAAGCTGGTGGAGGTCCTGGATGACCCCAATGAG; GACCATCTGTACATGGTGTTCGAACTGGTCAACCAAGGGCCCGTGATGGAAGTGCCCACC; CTCAAACCACTCTCTGAAGACCAGGCCCGTTTCTACTTCCAGGATCTGATCAAAGGCATC; GAGTACTTACACTACCAGAAGATCATCCACCGTGACATCAAACCTTCCAACCTCCTGGTC; GGAGAAGATGGGCACATCAAGATCGCTGACTTTGGTGTGAGCAATGAATTCAAGGGCAGT; GACGCGCTCCTCTCCAACACCGTGGGCACGCCCGCCTTCATGGCACCCGAGTCGCTCTCT; GAGACCCGCAAGATCTTCTCTGGGAAGGCCTTGGATGTTTGGGCCATGGGTGTGACACTA; TACTGCTTTGTCTTTGGCCAGTGCCCATTCATGGACGAGCGGATCATGTGTTTACACAGT; AAGATCAAGAGTCAGGCCCTGGAATTTCCAGACCAGCCCGACATAGCTGAGGACTTGAAG; GACCTGATCACCCGTATGCTGGACAAGAACCCCGAGTCGAGGATCGTGGTGCCGGAAATC; AAGCTGCACCCCTGGGTCACGAGGCATGGGGCGGAGCCGTTGCCGTCGGAGGATGAGAAC; TGCACGCTGGTCGAAGTGACTGAAGAGGAGGTCGAGAACTCAGTCAAACACATTCCCAGC; TTGGCAACCGTGATCCTGGTGAAGACCATGATACGTAAACGCTCCTTTGGGAACCCATTC; GAGGGCAGCCGGCGGGAGGAACGCTCACTGTCAGCGCCTGGAAACTTGCTCACCAAAAAA; CCAACCAGGGAATGTGAGTCCCTGTCTGAGCTCAAGGAAGCAAGGCAGCGAAGACAACCT; CCAGGGCACCGACCCGCCCCCCGTGGGGGAGGAGGAAGTGCTCTTGTGAGAGGCAGTCCC; TGCGTGGAAAGTTGCTGGGCCCCCGCCCCCGGCTCCCCCGCACGCATGCATCCACTGCGG; CCGGAGGAGGCCATGGAGCCCGAGTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172226 unedited; ACTCGGATTTGTAACCGACTTATATAGGCGGCCGCGCAATTCGCACGAGGGCCGAGCCGA; GCTGGGGGCGCAGAGCGCGGGAGGCGGCGGCGGCGCGGAGCCCAGGTGGCTCCGCTGCCG; GATGGGAGTGCCCCAGTGTGCTGGATGAAGCTGGCGCATGCACCATGTCATCATGTGTCT; CTAGCCAGCCCAGCAGCAACCGGGCCGCCCTTTTGTATGAGCTGGGGGGCAGGGGCAGCA; GCAGCAGCGAAAGCCAGAAGCCCTGTGAGGCCCTGCGGGGCCTCTCATCCTTGAGCATCC; ACCTGGGCATGGAGTCCTTCATTGTGGTCACCGAGTGTGAGCCGGGCTGTGCTGTGGACC; TCGGCTTGGCGCGGGACCGGCCCCTGGAGGCCGATGGCCAAGAGGTCCCCCTTGACACCT; CCGGGTCCCAGGCCCGGCCCCACCTCTCCGGTCGCAAGCTGTCTCTGCAAGAGCGGTCCC; AGGGTGGGCTGGCAGCCGGTGGCAGCCTGGACATGAACGGACGCTGCATCTGCCCGTCCC; TGCCCTACTCACCCGTCAGCTCCCCGCAGTCCTCGCCTCGGCTGCCCCGGCGGCCGACAG; TGGAGTCTCACCACGTCTCCATCACGGGTATGCAGGACTGTGTGCAGCTGAATCAGTATA; CCCTGGAAGATGAAATTGGAAAGGGCTCCTATGGTGTCGTCAAGTTGGCCTACAATGAAN; ATGAACATACCTACTATGCAATGAAAGTGCTGTCCAAAAAGAAGCTGATCCGGCAGGNNC; CGCTTCCACGTCGCCCTCCACCCCGAGGCACCCGGCCAGCTNCTGGNAGCTGCATCCAGC; CCNAGGGGCCCCATTGAGCAGGTGTACCAGGAAAATGCCATCCTCCAGAAGCTGGACCAC; CCCAATGTGGTGAAGCTGGTGGAAGTCCTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_172226 unedited; NNNCCTTACTCTGGACCGCGGGCCGCATGCTANGATCGAGTTTTTTTTTTTTTTTTTTTT; AAGTTCATGTACTTTTTAGTGATAAATACACAGTATTTAACTTTATACACCTGATAAAAG; GAAAATGCATAGTAGAAAGGGTCGGGAATGAAACAGAAGCATCGGGCATTGAATCGATCA; CAGAGTAGAAAAATCTGCAGTCCCCAGAAACTCGGCCTCCAGCTGGAGAGGGGAACCAGC; GTACCCTGAAACTTCCCTAGCACCTTGGAAGCAAGTGGAGGTCATGGTTCTTCCACTCGG; CAACGTGAAGCTCCCCGCTGGAAGACAAGTGAGAGCGACAGGCCAGGCCTGTGTGTCCAC; CTGCACAGGCATTCTCCTTGTTCCAGAAAGGCTCTGAGGACGGAAGACCTGCCTCTCAGG; AAAGGCCAGACCAGTAGGGAGAGGCCTCCGCAGCCCCAAGTGTCAACAAGGGGCTCAATA; AGGCTTTCTGGGAGCCACTGGCAGCTGGTGGGATGGAAGGGGGAGGTGGAAAAGGGCAGA; GGAAATGGGAAGTGGTGGGGGGTTTTGTGCTTCCTTCGAGGCCACAAGTGCAGGCCACAG; GTTGCTTACACCAAATTGGTCCCACAGATGAGCTAGCAATGGAAGAAATTATGGGGCGTC; CTCTTTGCCCACTGGAAGAGCTGCTCATAACAGCACCTCCTTTCCTGACAGAGCTGGCTT; TTGAAAAATCAATGTGCTCCAAGAGTGGCAGCTCAAATGCTCTGTGTTTACAGCCATCTT; GGCTTATCCTTTTCATGGGGGGCAGGGCCTTTCCCAGCTTTTTTTGGGGTGTCTTCATTA; AAGAAAAAAAAAGTCATCATTGTGGGTCTTCCCACCCACTGCCCCACAGCTGGGTGCTGN; CTGAATGGCTGCATTCCTGGAAGAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_172226
• Insert Size: 4860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_172226.1, NP_757380.1
• RefSeq Size: 4873 bp
• RefSeq ORF: 1626 bp
• Locus ID: 10645
• UniProt ID: Q96RR4
• Cytogenetics: 12q24.31
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway
• Summary: The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases. [provided by RefSeq, Jul 2012]; Transcript Variant: This variant (7) differs in the 5' UTR and also lacks a segment in the coding region, which leads to a frameshift, compared to variant 1. The resulting isoform (2) contains a shorter and distinct C-terminus compared to isoform 1. This isoform (2) is also encoded by variant 2.