Apolipoprotein L 1 (APOL1) (NM_003661) Human Untagged Clone

CAT#: SC109941

APOL1 (untagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Apolipoprotein L 1
• Synonyms: APO-L; APOL; APOL-I; FSGS4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109941 sequence for NM_003661 edited (data generated by NextGen Sequencing)
  ATGGAGGGAGCTGCTTTGCTGAGAGTCTCTGTCCTCTGCATCTGGATGAGTGCACTTTTC
  CTTGGTGTGGGAGTGAGGGCAGAGGAAGCTGGAGCGAGGGTGCAACAAAACGTTCCAAGT
  GGGACAGATACTGGAGATCCTCAAAGTAAGCCCCTCGGTGACTGGGCTGCTGGCACCATG
  GACCCAGAGAGCAGTATCTTTATTGAGGATGCCATTAAGTATTTCAAGGAAAAAGTGAGC
  ACACAGAATCTGCTACTCCTGCTGACTGATAATGAGGCCTGGAACGGATTCGTGGCTGCT
  GCTGAACTGCCCAGGAATGAGGCAGATGAGCTCCGTAAAGCTCTGGACAACCTTGCAAGA
  CAAATGATCATGAAAGACAAAAACTGGCACGATAAAGGCCAGCAGTACAGAAACTGGTTT
  CTGAAAGAGTTTCCTCGGTTGAAAAGTGAGCTTGAGGATAACATAAGAAGGCTCCGTGCC
  CTTGCAGATGGGGTTCAGAAGGTCCACAAAGGCACCACCATCGCCAATGTGGTGTCTGGC
  TCTCTCAGCATTTCCTCTGGCATCCTGACCCTCGTCGGCATGGGTCTGGCACCCTTCACA
  GAGGGAGGCAGCCTTGTACTCTTGGAACCTGGGATGGAGTTGGGAATCACAGCCGCTTTG
  ACCGGGATTACCAGCAGTACCATGGACTACGGAAAGAAGTGGTGGACACAAGCCCAAGCC
  CACGACCTGGTCATCAAAAGCCTTGACAAATTGAAGGAGGTGAAGGAGTTTTTGGGTGAG
  AACATATCCAACTTTCTTTCCTTAGCTGGCAATACTTACCAACTCACACGAGGCATTGGG
  AAGGACATCCGTGCCCTCAGACGAGCCAGAGCCAATCTTCAGTCAGTACCGCATGCCTCA
  GCCTCACGCCCCCGGGTCACTGAGCCAATCTCAGCTGAAAGCGGTGAACAGGTGGAGAGA
  GTTAATGAACCCAGCATCCTGGAAATGAGCAGAGGAGTCAAGCTCACGGATGTGGCCCCT
  GTAAGCTTCTTTCTTGTGCTGGATGTAGTCTACCTCGTGTACGAATCAAAGCACTTACAT
  GAGGGGGCAAAGTCAGAGACAGCTGAGGAGCTGAAGAAGGTGGCTCAGGAGCTGGAGGAG
  AAGCTAAACATTCTCAACAATAATTATAAGATTCTGCAGGCGGACCAAGAACTGTGA
  
  Clone variation with respect to NM_003661.3
  764 g=>a;960 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003661 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCAGAACAGCTGGATCTTGC
  TCAGTCTCTGCCAGGGGAAGATTCCTTGGAGGAGGCCCTGCAGCGACATGGAGGGAGCTG
  CTTTGCTGAGAGTCTCTGTCCTCTGCATCTGGATGAGTGCACTTTTCCTTGGTGTGGGAG
  TGAGGGCAGAGGAAGCTGGAGCGAGGGTGCAACAAAACGTTCCAAGTGGGACAGATACTG
  GAGATCCTCAAAGTAAGCCCCTCGGTGACTGGGCTGCTGGCACCATGGACCCAGAGAGCA
  GTATCTTTATTGAGGATGCCATTAAGTATTTCAAGGAAAAAGTGAGCACACAGAATCTGC
  TACTCCTGCTGACTGATAATGAGGCCTGGAACGGATTCGTGGCTGCTGCTGAACTGCCCA
  GGAATGAGGCAGATGAGCTCCGTAAAGCTCTGGACAACCTTGCAAGACAAATGATCATGA
  AAGACAAAAACTGGCACGATAAAGGCCAGCAGTACAGAAACTGGTTTCTGAAAGAGTTTC
  CTCGGTTGAAAAGTAAGCTTGAGGATAACATAAGAAGGCTCCGTGCCCTTGCAGATGGGG
  TTCAGAAGGTCCACAAAGGCACCACCATCGCCAATGTGGNTGTCTGGCTCTCTCAGCATT
  TCCTCTGGCATCCTGACCCTCGTCGGCATGGGTTTGGCACCCTTCACAGAGGGAGGCAGC
  CTTGTACTCTTGGAACCTGNGNNATGGAGTGGNAATCACAGCAGCTTTGACCGGGGATAC
  CAGCAGTACATAGACTACGNAANGAAGTGGTGGGACACAGCCCAAGCCCACGACTGGTCA
  TCANAAGCTTGACANNATGANGAGTGAANGGGAGTTTTGGGTGAGACATTATCACTTTCT
  TTNCTAGCTGGCATACTTACACTACACGAGCATGGGAGGGACTNCTGCCTAGACAGCAAG
  CATTTTATCATACGATGCTAGCTACCCCCGGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003661 unedited
  CTATGGCACGCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTGAGAGGAGTC
  TCGCTCTGTCACCCAGGCTGGAGTGCAGTGGCGATATCTCGGCTCACTGCAAGCTCCACC
  TCCCAGGCTCACGCCATTCTCCTGCCTCAGCCGCCCAGTCAGCCGGAACCACAGGCGCCC
  GCCACCATGCCCGGCCAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGTGCTAGC
  CAGGATGGCCTCGATCTCCTGACCTCGTGATCCGCCCGCCTTGGCCTCCCAAAGTGCCCG
  GACCACAGGCATGAGCCACTGCGCCCGGACCCCCAATATATTCTTTAGTCTAAAGCAAAC
  TGCTTCTTCCCATTCCCCACACTCTGCAGTTCCCATTTTTCCCTTATTGCAGGCTCAAAT
  GTTCCTGCCTTATACTTGCTGCACTGCCATCTGCATTAACCCCTCCCCTGCTGTGCTCAC
  CTATGGAAATGCCAAAGCCCCTAACACTCAGTTCCATAAACTTTACCTGACCCTCTTTAT
  CCCCCTAATAAAATGTTTGCATTTGGGCCAAAACCGATGGCTCACGCCTGTAATCCCACT
  ACTTTGGGAGGCCAAGGTGGGCATATCAACAGGCCAGGAGATCGACACCATCCTGGCGCA
  CACGGCCGAAACCCCATCTCTATTAAAAATTCCCAAATTACCTGGGCAGGCNGGCAACCG
  CCTGTTTACTCAACTACTTGGGCAGGCCAAGGCAGGAGAATCCGCTTGACATGGGAAGAC
  ACCCCTGTCTGGACCCAGATCGCCCCACTGCACTCCACTTTGCGAACAGACAAGACTTTG
  GCTCAAACAAAAAAAAATAGTTTGCTTTTGGCCTGCGCCCCGCCAGCTTATCTTTCTGGG
  GGTGGACTGCCCGGAGCCCACATCCGCCCCCCCTCTCAATATTTAACCCCCCATAATCTC
  CCCTCCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003661
• Insert Size: 2140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003661.2, NP_003652.2
• RefSeq Size: 2856 bp
• RefSeq ORF: 1197 bp
• Locus ID: 8542
• UniProt ID: O14791
• Cytogenetics: 22q12.3
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
  Transcript Variant: This variant (1) encodes the predominant protein (isoform a). Variants 1 and 3 encode the same protein (isoform a). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.