Von Hippel Lindau (VHL) (NM_000551) Human Untagged Clone

SKU SC109881

VHL (untagged)-Human von Hippel-Lindau tumor suppressor (VHL), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Von Hippel Lindau
• Synonyms: HRCA1; pVHL; RCA1; VHL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000551 edited; ATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTC; GAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCG; GAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGGCGGCCGCGG; CCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGT; CCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACG; CTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGA; GATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTC; AATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAG; CGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATC; GTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGG; CTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000551 unedited; GGCTCAGATTGTAAACGACTTACTGTGGGCGGCAAAGCCCTTCGCACGAGCCTCGTGCCT; ATTTCGGCACGAGGCCGCGGCGTCCCGCCCGGGTGGTCTGGATCGCGGAGGGAATGCCCC; GGAGGGCGGACAACTGGGACGAGGCCGAGGTAAGCGCGGAGGAGGCAGGCGTCGAAGAGT; ACGGCCCTGCAACAAGACGGCGGGGAGGAGTTGTTTTTCTGACGAGTCCGGCCCGGAAGA; GTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGATATGGAGGCCGGGCGGCCGCGGCCCGT; GCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCG; CGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCC; GCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAAAGATGC; AGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGT; TGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATG; CCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAG; GTCGCTCTACGAAGATCTGGAAGACCACCCATATGTGCAGAAAGACCTGGAGCGGCTGAC; ACAGGAGCGCATTGCACATCACCGGATGGGAGATTGAAGATTTCTGTTGAAACTTACACT; GGTTCATCTCAACTTTTGATGGGACTGGTGAGTCTTGATCTAGATACAGGACTGGGTTCT; TTCTTAAGTTTCAAAGGGTCTCATTCTCAGAGTTAAATAGGCCCCATTGCTTAAAAGGAA; ATTAACGGCCTTCCCTAGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000551 unedited; CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGAGGCAGGGTCTTGCCCT; GTCCCCCAGGCTGGAGTGCAGTGGCATACTCATGGCTCACTGCCGCCTCCAATTCCTAAA; CTCCAATGATCTTCTCACCTCAGCCTGCCAAGTAGCTAGGATTACAGATGTGTGCCGCCA; TGCTAATTTTTAAAAATTTTTTGTATAGATGGGTTCTCACTATGAGCCCAGGCTCATCTT; GAAGTTCTGGGCTCAAGCAATCCTCTCACCTTGGCTTCCCAAAGTACTGGGATTACAGGC; GGGAGCCACCATGCCAGCCGTCACTCTCATTTTTGAGCACAGGAATTGTGCCCAAAGGCT; AGGATAAGGAATTAAAGATGCATGCCAAGAAATACCCACAAACATTAATTATAAAAGGGA; GACTATACTAATGTTTGTGAAAAGCTTATACTAACATAGAGCAATATTTAAGTTATAAAG; TTAAATGAAAAAGGGTATAAAACTGTATGAACAGTACAATCTCAACTATGTAAAAACCAA; CCAAAATCTGCCCTAAAAAGGCTGGAAGGAAATAGCTCAAAATGTTAATGTGATTGTATT; TAAGAAGTGTATCTAATAGGGATCTTTTTTCTTCCTTTTTAGCTCTCTGCCCTTTCCAAA; TGTTTCTCTACTGGGGAGATTTTTTTAAAAAGCTCTTAGGGAAGGAGTACCAACCAAAGT; GGAAAAAAAAATCTATTATTTACCAAAAACTGAGTCTGAAAAAACCATTGAATTTGAGGT; CATGACTCCAAGTCTACCCTTAAAGAATTTGCGCATCTATAAACCTTGGAACCGTCCCGC; TTCCTCATGAATCTCTGTTAGACCAAAGCTGAAGTGGAACGCCCCTGGCCTGCTGAAAAA; AAGCTGATAGCCGGCCGGGGCCCCACCCGGTTCCCCCTTTCGGAGCTGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000551
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000551.2, NP_000542.1
• RefSeq Size: 2968 bp
• RefSeq ORF: 642 bp
• Locus ID: 7428
• UniProt ID: P40337
• Cytogenetics: 3p25.3
• Domains: VHL
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis
• Summary: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.