CHKL (CHKB) (NM_152253) Human Untagged Clone

SKU SC109066

CHKB (untagged)-Human choline kinase beta (CHKB), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: CHKL
• Synonyms: CHETK; CHKL; choline/ethanolamine kinase; choline kinase-like; choline kinase beta; CKEKB; EKB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109066 sequence for NM_152253 edited (data generated by NextGen Sequencing); ATGGCGGCCGAGGCGACAGCTGTGGCCGGAAGCGGGGCTGTTGGCGGCTGCCTGGCCAAA; GACGGCTTGCAGCAGTCTAAGTGCCCGGACACTACCCCAAAACGGCGGCGCGCCTCGTCG; CTGTCGCGTGACGCCGAGCGCCGAGCCTACCAATGGTGCCGGGAGTACTTGGGCGGGGCC; TGGCGCCGAGTGCAGCCCGAGGAGCTGAGGGTTTACCCCGTGAGGTGGGAGGTCAGGGGT; CAGCCTCTCCGGTGCGCGGATCGGGGTCAGGGGTCAGCCGCGGGGCCCTCAGGATGCTCC; ATGTTTTCGCCCCCCTCTTGCGCCCGCGCCTGGGGCGGGGCGGGGCCGGCCTGGCCGGGA; GGGGGCCGGGGCCGCGGCAGGTAG; ; Clone variation with respect to NM_152253.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152253 unedited; TTGGATTATGTAACACGATCTTATATAGGCGGCCGCGCAATTCGCACCAGGNAAAAAGCC; CCCGGGCCGGGGCACGGAGAGAGCCGAGCGCCGCAGCCGTGAGCCGAATAGAGCCGGAGA; GACCCGAGTATGACCGGAGAAGCCCAGGCCGGCCGGAAGAGGAGCCGAGCGCGGCCGGAA; GGAACCGAGCCCGTCCGAAGGGAGCGGAGCGCAGCCTGGCCTGGGGCCCGGTCGAGCCCG; CGCCATGGCGGCCGAGGCGACAGCTGTGGCCGGAAGCGGGGCTGTTGGCGGCTGCCTGGC; CAAAGACGGCTTGCAGCAGTCTAAGTGCCCGGACACTACCCCAAAACGGCGGCGCGCCTC; GTCGCTGTCGCGTGACGCCGAGCGCCGAGCCTACCAATGGTGCCGGGAGTACTTGGGCGG; GGCCTGGCGCCGAGTGCAGCCCGAGGAGCTGAGGGTTTACCCCGTGAGGTGGGAGGTCAG; GGGTCAGCCTCTCCGGTGCGCGGATCGGGGTCAGGGGTCAGCCGCGGGGCCCTCAGGATG; CTCCATGTTTTCGCCCCCCTCTTGCGCCCGCGCCTGGGGCGGGGCGGGGCCGGCCTGGCC; GGGAGGGGGCCGGGGCCGCGGCAGGTAGGGCCGGCCGCGGGCTGAGCGCGCCTGGTGTGG; GTCTGCAGCGGGAGCCTCAGCAACCTGCTCTTCCGCTGCTCGCTCCCGGACCACCTGCCC; AGCGTTGGCGAGGAGCCCCGGGAGGTGCTTCTGCNGCTGTACGGAGCCATCTTGCANNGT; GAGGGGTTGTGAGCGCCGCAGCACCAGTGGCTTTAGGGCCTGTCGCTTACGCGATGCGGG; TAGTATTGGTCCCGTTGCGCAGTTGAGGACACCGAGTTCACGGTCTGAGTAACACTCATT; ACACGAAGGCTGGGGCTGTATCCCAGAGCTTTNGGAGCTGNAGGAGAGGATCACTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_152253
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152253.1, NP_689466.1
• RefSeq Size: 4914 bp
• RefSeq ORF: 384 bp
• Locus ID: 1120
• Cytogenetics: 22q13.33
• Domains: Carn_acyltransf
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Summary: Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]; Transcript Variant: This variant (2) contains alternate segments in the coding region which causes a frameshift, compared to variant 1. The resulting protein (isoform b) is shorter and has a distinct C-terminus than isoform a. In addition, this variant is bicistronic, with the coding region of a carnitine palmitoyltransferase gene present in the 3' end of the transcript.