CDK2 (NM_001798) Human Untagged Clone

CAT#: SC109060

CDK2 (untagged)-Human cyclin-dependent kinase 2 (CDK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK2
• Synonyms: CDKN2; p33(CDK2)
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001798 edited
  CGGCCGCGAATTCGGCACGAGGCAACATTGTTTCAAGTTGGCCAAATTGACAAGAGCGAG
  AGGTATACTGCGTTCCATCCCGACCCGGGGCCACGGTACTGGGCCCTGTTTCCCCCTCCT
  CGGCCCCCGAGAGCCAGGGTCCGCCTTCTGCAGGGTTCCCAGGCCCCCGCTCCAGGGCCG
  GGCTGACCCGACTCGCTGGCGCTTCATGGAGAACTTCCAAAAGGTGGAAAAGATCGGAGA
  GGGCACGTACGGAGTTGTGTACAAAGCCAGAAACAAGTTGACGGGAGAGGTGGTGGCGCT
  TAAGAAAATCCGCCTGGACACTGAGACTGAGGGTGTGCCCAGTACTGCCATCCGAGAGAT
  CTCTCTGCTTAAGGAGCTTAACCATCCTAATATTGTCAAGCTGCTGGATGTCATTCACAC
  AGAAAATAAACTCTACCTGGTTTTTGAATTTCTGCACCAAGATCTCAAGAAATTCATGGA
  TGCCTCTGCTCTCACTGGCATTCCTCTTCCCCTCATCAAGAGCTATCTGTTCCAGCTGCT
  CCAGGGCCTAGCTTTCTGCCATTCTCATCGGGTCCTCCACCGAGACCTTAAACCTCAGAA
  TCTGCTTATTAACACAGAGGGGGCCATCAAGCTAGCAGACTTTGGACTAGCCAGAGCTTT
  TGGAGTCCCTGTTCGTACTTACACCCATGAGGTGGTGACCCTGTGGTACCGAGCTCCTGA
  AATCCTCCTGGGCTGCAAATATTATTCCACAGCTGTGGACATCTGGAGCCTGGGCTGCAT
  CTTTGCTGAGATGGTGACTCGCCGGGCCCTATTCCCTGGAGATTCTGAGATTGACCAGCT
  CTTCCGGATCTTTCGGACTCTGGGGACCCCAGATGAGGTGGTGTGGCCAGGAGTTACTTC
  TATGCCTGATTACAAGCCAAGTTTCCCCAAGTGGGCCCGGCAAGATTTTAGTAAAGTTGT
  ACCTCCCCTGGATGAAGATGGACGGAGCTTGTTATCGCAAATGCTGCACTACGACCCTAA
  CAAGCGGATTTCGGCCAAGGCAGCCCTGGCTCACCCTTTCTTCCAGGATGTGACCAAGCC
  AGTACCCCATCTTCGACTCTGATAGCCTTCTTGAAGCCCCCAGCCCTAATCTCACCCTCT
  CCTCCAGTGTGGGCTTGACCAGGCTTGGCCTTGGGCTATTTGGACTCAGGTGGGCCCTCT
  GAACTTGCCTTAAACACTCACCTTCTAGTCTTGGCCAGCCAACTCTGGGAATACAGGGGT
  GAAAGGGGGGGAACCAGTGAAAATGAAAGGAAGTTTCAGTATTAGATGCACTTAAGTTAG
  CCTCCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001798 unedited
  TGTAATACGACTCACTATAGGGNNNCGGCCGCGAATTCGGCACGAGGCAACATTGTTTCA
  AGTTGGCCAAATTGACAAGAGCGAGAGGTATACTGCGTTCCATCCCGACCCGGGGCCACG
  GTACTGGGCCCTGTTTCCCCCTCCTCGGCCCCCGAGAGCCAGGGTCCGCCTTCTGCAGGG
  TTCCCAGGCCCCCGCTCCAGGGCCGGGCTGACCCGACTCGCTGGCGCTTCATGGAGAACT
  TCCAAAAGGTGGAAAAGATCGGAGAGGGCACGTACGGAGTTGTGTACAAAGCCAGAAACA
  AGTTGACGGGAGAGGTGGTGGCGCTTAAGAAAATCCGCCTGGACACTGAGACTGAGGGTG
  TGCCCAGTACTGCCATCCGAGAGATCTCTCTGCTTAAGGACGCTTAACCATCCTAATATT
  GTCAAGCTGCTGGATGTCATTCACACAGAAAATAAACTCTACCTGGTTTTTGAATTTCTG
  CACCAAGATCTCAAGAAATTCATGGATGCCTCTGCTCTCACTGGCATTCCTCTTCCCCTC
  ATCAAGAGCTATCTGTTCCAGCTGCTCCAGGGCCTAGCTTTCTGCCATTCTNCATCGGTC
  CTCCACCGAGACCTTANACCTCAGAATCTGCTTATTAACACAGAGGGGGCCATCAAGCTA
  GCAGACTTTGGACTAACCAGAGCTTTTGGAGTCCCTGTTCGTACTTACACCCATGAGGTG
  GTGACCCTGTGGTCCCGAGCNTCCTGAAATCCTCCTGGGCTGNCAATATTATCCCACAGC
  TTGTGGACATCTTGGAGCCTGGGCTGCATCTTTGCTGAGATGGTGACTTCGCCGCCCCTA
  TTCCCTGGAGATTCTGAGATTGACCAGCTCTTCCGGTATCTTCNGACTCTGGNGACCCAN
  ATGAGGTGGTGTGGCCAGNAATACTCNATGNCTGATACAGCCAGTTTCCCAGTGGCCCGC
  AG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001798 unedited
  TGCCATTTTTTTAAGATTTACAAACTAAATTACAAATAACGTTAATAGCAAGAGCACTCA
  AGTACAGGGTGACAGAGACAAATTATAAAAAAAAAAGCCCTTTTTTCTTTAGTACTGCCA
  TTACAAAATCTCAATGTGCACATGGTAACAAATCCACCCACTATGACCAAAAAAAAAAAA
  AAAATCTAACTTTACTATTGGATATAACCCCAAATATCAATACAATCGCCTATGCTCCCT
  TTAAACAAATATTCCCACCCTGCCCTCTCTCTCTCCCCATCTTCAAAAATCGCCATTATT
  CTTTACAATGATGCTCAACTTTTACTTTTAACTTTCTATGGCGTCTCTCTTCTTTCCTCC
  TTTTCTTTTTTTCTCCTTTCCCTTTCCCCCTTTTCCCCCCTTCCATCTTTTCCCCCCCCT
  TTTTCCCCCTTCCCCCTTTCCCCCCTCCCCTCCCCCCCCTTTTCCCTTTTTCCCCATCCA
  TTTTTTCCCCCATTTCCCCCCCCTTTTATTTTCCCTTTTCTTCCCTTCATTTCCCCCCCT
  CCTTTTTCCATTTCCCTCCTCTTCCCTTTTTTCCCTTCCCTCCCCCCTCCCCCTTTTCTT
  TTCCCCTTCTTTCCCCCCCCCCCCCTTTCCCTCCCCCCCCCCTCCCCCTTTTTCTTCCCC
  CCCTTTCCCCCCCCCTTCCTACTTATCCTTATTTTTTCCTTCCCCCATCCCCCCCCCCCC
  TTTTTTCCACCTTTTTCTCTCCCCCCCCCCCCTTTTTCCCCCCCCCCCTCTCCGCTTTCC
  CTTCCCTTTCTCCCCCTTTCCCCTCCCTTTCTTTTCACCCCATTCCCCTACCCCTCTCTC
  TTCCTTTTTTCCTTATTCTCCCCCCACCCTTTTCTTTTTTTTTTACTCCCTCCCCTGTCC
  CCCCCTTTTCTTTCTTCCCCCCTGTTTCTTTATCCCCCCCCTATTCCCCTCTTCCCTTTT
  CCCGCTCACCCTTTCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001798
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001798.2, NP_001789.2
• RefSeq Size: 2328 bp
• RefSeq ORF: 897 bp
• Locus ID: 1017
• UniProt ID: P24941
• Cytogenetics: 12q13.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Small cell lung cancer
• Gene Summary: This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).