Beta Arrestin 2 (ARRB2) (NM_004313) Human Untagged Clone

SKU SC108950

ARRB2 (untagged)-Human arrestin, beta 2 (ARRB2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Beta Arrestin 2
• Synonyms: ARB2; ARR2; BARR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004313 edited; GAATTCGGCACGAGGTGGCAGCGGGCGAGGAGGCTGCGAGCGAGCCGCGAACCGAGCGGG; CGGCGGGCGCGCGCACCATGGGGGAGAAACCCGGGACCAGGGTCTTCAAGAAGTCGAGCC; CTAACTGCAAGCTCACCGTGTACTTGGGCAAGCGGGACTTCGTAGATCACCTGGACAAAG; TGGACCCTGTAGATGGCGTGGTGCTTGTGGACCCTGACTACCTGAAGGACCGCAAAGTGT; TTGTGACCCTCACCTGCGCCTTCCGCTATGGCCGTGAAGACCTGGATGTGCTGGGCTTGT; CCTTCCGCAAAGACCTGTTCATCGCCACCTACCAGGCCTTCCCCCCGGTGCCCAACCCAC; CCCGGCCCCCCACCCGCCTGCAGGACCGGCTGCTGAGGAAGCTGGGCCAGCATGCCCACC; CCTTCTTCTTCACCATACCCCAGAATCTTCCATGCTCCGTCACACTGCAGCCAGGCCCAG; AGGATACAGGAAAGGCCTGCGGCGTAGACTTTGAGATTCGAGCCTTCTGTGCTAAATCAC; TAGAAGAGAAAAGCCACAAAAGGAACTCTGTGCGGCTGGTGATCCGAAAGGTGCAGTTCG; CCCCGGAGAAACCCGGCCCCCAGCCTTCAGCCGAAACCACACGCCACTTCCTCATGTCTG; ACCGGTCCCTGCACCTCGAGGCTTCCCTGGACAAGGAGCTGTACTACCATGGGGAGCCCC; TCAATGTAAATGTCCACGTCACCAACAACTCCACCAAGACCGTCAAGAAGATCAAAGTCT; CTGTGAGACAGTACGCCGACATCTGCCTCTTCAGCACCGCCCAGTACAAGTGTCCTGTGG; CTCAACTCGAACAAGATGACCAGGTATCTCCCAGCTCCACATTCTGTAAGGTGTACACCA; TAACCCCACTGCTCAGTGACAACCGGGAGAAGCGGGGTCTCGCCCTGGATGGGAAACTCA; AGCACGAGGACACCAACCTGGCTTCCAGCACCATCGTGAAGGAGGGTGCCAACAAGGAGG; TGCTGGGAATCCTGGTGTCCTACAGGGTCAAGGTGAAGCTGGTGGTGTCTCGAGGCGGGG; ATGTCTCTGTGGAGCTGCCTTTTGTTCTTATGCACCCCAAGCCCCACGACCACATCCCCC; TCCCCAGACCCCAGTCAGCCGCTCCGGAGACAGATGTCCCTGTGGACACCAACCTCATTG; AATTTGATACCAACTATGCCACAGATGATGACATTGTGTTTGAGGACTTTGCCCGGCTTC; GGCTGAAGGGGATGAAGGATGACGACTATGATGATCAACTCTGCTAGGAAGCGGGGTGGG; AAGAAGGGAGGGGATGGGGTTGGGAGAGGTGAGGGCAGGATTAAGATCCCCACTGTCAAT; GGGGGATTGTCCCAGCCCCTCTTCCCTTCCCCTCACCTGGAAGCTTCTTCAACCAATCCC; TTCACACTCTCTCCCCCATCCCCCCAAGATACACACTGGACCCTCTCTTGCTGAATGTGG; GCATTAATTTTTTGACTGCAGCTCTGCTTCTCCAGCCCCGCCGTGGGTGGCAAGCTGTGT; TCATACCTAAATTTTCTGGAAGGGGACAGTGAAAAGAGGAGTGACAGGAGGGAAAGGGGG; AGACAAAACTCCTACTCTCAACCTCACACCAACACCTCCCATTATCACTCTCTCTGCCCC; CATTCCTTCAAGAGGAGACCCTTTGGGGACAAGGCCGTTTCTTTGTTTCTGAGCATAAAG; AAGAAAATAAATCTTTTACTAAGAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004313 unedited; TGTCAGAAATTTTGTATACGAACTCACTATAGGGCGGCCGCGATTCGGCACGAGGTGGCA; GCGGGCGAGGAGGCTGCGAGCGAGCCGCGAACCGAGCGGGCGGCGGGCGCGCGCACCATG; GGGGAGAAACCCGGGACCAGGGTCTTCAAGAAGTCGAGCCCTAACTGCAAGCTCACCGTG; TACTTGGGCAAGCGGGACTTCGTAGATCACCTGGACAAAGTGGACCCTGTAGATGGCGTG; GTGCTTGTGGACCCTGACTACCTGAAGGACCGCAAAGTGTTTGTGACCCTCACCTGCGCC; TTCCGCTATGGCCGTGAAGACCTGGATGTGCTGGGCTTGTCCTTCCGCAAAGACCTGTTC; ATCGCCACCTAACCAGGCCTTCCCCCCCGGTGCCCAACCCACCCCGGCCCCCCACCCGCC; TGCAGGACCGGCTGCTGAGGAAGCTGGGCCAGCATGCCCACCCCTTCTTCTTCACCATAC; CCCAGAATCTTCCATGCTCCGTCACACTGCAGCCAGGCCCAGAGGATACAGGAAAGGCCT; GCGGCGTAGACTTTGAGATTCGAGCCTTCTGTGCTAAATCACTAGAAGAGAAAAGCCACA; AAAGGAACTCTGTGCGGCTGGTGATCCGAAAGGTGCAGTTCGCCCCGGAGAAACCCGGCC; CCCAGCCTTCAGCCGAAACCACACGCCACTTCCTCATGTCTGACCGGTCCCTGCACCTCG; AGGCTTCCCTGGACAGGGAGCTGTACTACCATGGNGAGCCCCTCATGTANATGTCCACGT; CACCAACAACTCCACCAAGACCGTNCAGAAGATCANAGTCTCTGTGAGACAGTACGCCGA; CATCTGCCTCTTCAGCACCGCCCAGTACAAGTGTCCTGTGGCTCAACTCGAACAGAATGA; CCAGTATCTCCCCAGCTCCATA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004313 unedited; GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTCTTAGTAAAAGATTTATTTTTC; TTCTTTATGCTCAGAAACAAAGAAACGGCCTTGTCCCCAAAGGGTCTCCTCTTGAAGGAA; TGGGGGCAGAGAGAGTGATAATGGGAGGTGTTGGTGTGAGGTTGAAAGTAGGAGTTTTGT; CTCCCCCTTTCCCTCCTGTCACTCCTCTTTTCACTGTCCCCTTCCAGAAAATTTAGGTAT; GAACACAGCTTGCCACCCACGGCGGGGCTGGAGAAGCAGAGCTGCAGTCAAAAAATTAAT; GCCCACATTCAGCAAGAGAGGGTCCAGTGTGTATCTTGGGGGGATGGGGGAGAGAGTGTG; AAGGGATCTGGTTTGAAGAAGCTTCCAGGTGAGGGGAAGGGAAGAGGGGCTGGGACAATC; CCCCATTGACAGTGGGGATCTTAATCCTGCCCTCACCTCTCCCAACCCCATCCCCTCCCT; TCTTCCCACCCCGCTTCCTAGCAGAGTTGATCATCATAGTCGTCATCCTTCATCCCCTTC; AGCCGAAGCCGGGCAAAGTCCTCAAACACAATGTCATCATCTGTGGCATAGTTGGTATCA; AATTCAATGAGGTTTGGTGTCCACAGGGACATCTGTCTCCGGAGCGGCTGACTGGGGTCT; GAGGATGGGGNATGCTGGTCNTGGGGGCTGGGGGTGCATAANAACAAAGGCATCTGCCAG; AGACATCCCCGCCTCGAGACACACAAGTTTAACCTAGACCCTGTAAGACACCAGGATTCC; CAGCACTTCCTTGNTGGCACCCTNCTCACGATGGTGCTGGAAGCCAGGTTGGTGTCCTCG; AGCTTGAGTTTGCATTCAGGGCGATACCCCGCTCTCCCGGTTGCACTGGCCATGGGGGTT; GGGGGTCCCCTACAAATTGGGAGCGGGAATCCCGGCATCTTGTCAATTGACCCAGGCCTT; GTCCTGGCGGGCGAAGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004313
• Insert Size: 1880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004313.3, NP_004304.1
• RefSeq Size: 1936 bp
• RefSeq ORF: 1230 bp
• Locus ID: 409
• UniProt ID: P32121
• Cytogenetics: 17p13.2
• Domains: arrestin
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Endocytosis, MAPK signaling pathway, Olfactory transduction
• Summary: Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]; Transcript Variant: This variant (1) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 3. The resulting isoform (1) lacks an alternate internal segment compared to isoform 3.