Chromosome X open reading frame 6 (MAMLD1) (NM_005491) Human Untagged Clone

SKU SC108540

MAMLD1 (untagged)-Human mastermind-like domain containing 1 (MAMLD1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Chromosome X open reading frame 6
• Synonyms: CG1; CXorf6; F18; HYSP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005491 edited; ATGGATGACTGGAAAAGTCGGCTTGTAATCAAGAGCATGCTTCCCCATTTCGCCATGGTG; GGAAATCGTCAGGAGCCCAGAAAGCTCCAGGAATCGGGAAAGAAGCCCTCGTGGATGGAG; GAAGAAGATTTATCTTTTCTCTACAAGAGCAGCCCAGGAAGAAAGCATCAGGGAACTGTT; AAGAGGAGACAAGAAGAAGACCACTTCCAGTTTCCAGACATGGCTGATGGGGGCTACCCT; AATAAAATTAAGAGGCCTTGCCTTGAAGATGTCACCCTTGCAATGGGCCCAGGTGCTCAT; CCTAGTACTGCTTGTGCAGAACTGCAGGTCCCTCCATTGACAATAAATCCTAGCCCTGCG; GCTATGGGAGTGGCTGGCCAGTCATTACTGCTGGAGAATAACCCTATGAATGGCAACATC; ATGGGCTCACCATTTGTAGTACCACAGACTACAGAAGTGGGACTGAAAGGGCCCACTGTT; CCTTACTATGAGAAAATCAACAGCGTGCCGGCTGTAGACCAGGAGCTTCAAGAGCTGCTA; GAGGAGCTCACCAAAATTCAAGACCCTTCTCCAAATGAGCTAGATCTTGAGAAGATACTG; GGGACGAAGCCAGAAGAGCCACTGGTTTTAGATCATCCCCAGGCAACCCTAAGCACAACT; CCCAAGCCTTCGGTTCAGATGTCACACTTGGAGAGCCTGGCTTCCAGCAAGGAGTTTGCT; TCTAGTTGCAGCCAAGTTACTGGCATGTCACTTCAGATCCCATCCTCCTCCACAGGGATC; AGCTATTCGATTCCTTCCACCAGTAAGCAGATAGTGTCACCGAGTTCTTCAATGGCACAG; TCCAAGAGCCAGGTCCAGGCCATGCTCCCTGTCGCTCTGCCCCCCTTACCAGTGCCTCAG; TGGCATCACGCCCACCAGCTGAAGGCGTTGGCAGCCAGCAAGCAGGGGTCTGCTACAAAG; CAGCAAGGGCCCACCCCCAGTTGGTCTGGTCTGCCTCCTCCAGGACTCTCTCCACCTTAC; CGCCCAGTGCCATCACCACACCCACCACCGCTGCCACTGCCACCACCACCACCCCCATTC; AGCCCCCAGAGCCTCATGGTGTCCTGCATGTCGTCCAATACCTTGTCGGGTAGCACTCTC; CGAGGCTCTCCCAATGCCTTACTGTCAAGCATGACGTCCAGCAGCAATGCTGCCCTGGGG; CCCGCCATGCCCTATGCTCCTGAGAAGCTCCCCAGCCCTGCTCTCACTCAACAGCCGCAG; TTCGGCCCTCAGAGCTCCATTCTTGCCAACCTCATGTCCTCTACCATCAAAACCCCTCAA; GGACACCTGATGTCTGCTCTGCCTGCCAGCAACCCTGGGCCGTCCCCACCCTATCGCCCA; GAGAAGCTCTCTAGCCCAGGCTTGCCACAGCAGTCCTTCACCCCACAGTGTTCCCTGATC; CGAAGCCTCACTCCCACCAGTAATCTTCTAAGCCAGCAACAGCAGCAGCAGCAGCAGCAG; CAGCAAGCAAATGTGATCTTTAAGCCCATAAGCAGCAACTCATCCAAAACCCTGAGCATG; ATCATGCAGCAGGGGATGGCAAGCTCCAGCCCAGGAGCCACGGAGCCATTTACTTTTGGC; AACACCAAGCCCTTGTCCCATTTTGTTTCTGAGCCGGGTCCCCAGAAGATGCCCTCCATG; CCTACCACCTCTAGGCAGCCTTCCCTGCTCCACTACCTGCAGCAGCCGACACCAACGCAG; GCCTCCTCAGCCACTGCCTCCTCCACGGCCACTGCCACCTTGCAGCTGCAGCAGCAGCAG; CAGCAACAGCAGCAGCAGCCTGACCATTCTTCATTCCTTCTGCAGCAGATGATGCAGCAA; CCCCAGCGTTTTCAGCGATCAGTGGCCTCAGATTCCATGCCTGCTCTGCCCAGACAGGGC; TGTTGCCATCTGTTTGCATGGACTTCTGCAGCTAGCTCGGTGAAGCCCCAGCATCAACAC; GGGAACTCTTTCACTAGCAGGCAAGATCCTCAGCCTGGAGACGTGTCACCGTCTAACATT; ACTCATGTAGACAAAGCCTGCAAGCTTGGGGAAGCCAGGCACCCCCAGGTCAGCCTCGGG; CGACAGCCCCCCTCCTGCCAGGCCCTGGGGAGTGAGTCCTTCCTGCCCGGCAGCTCCTTT; GCTCATGAGCTGGCCCGAGTCACCTCCTCGTACAGCACCTCAGAGGCAGCGCCCTGGGGC; AGCTGGGATCCGAAGGCCTGGAGGCAGGTGCCCGCTCCACTACTGCCTAGCTGCGACGCC; ACAGCAAGAGGAACAGAGATCAGGTCTTATGGCAATGACCCCTGA;
• Restriction Sites: Please inquire
• ACCN: NM_005491
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone has been fully sequenced and found three SNPs within the protein associated with this reference, NM_005491.1:At position 2422, a C to G change results in no amino acid change.At position 2354, a A to C change results a H to N change.At position 2343, a A to C change results in no amino acid change.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005491.1, NP_005482.1
• RefSeq Size: 4599 bp
• RefSeq ORF: 2106 bp
• Locus ID: 10046
• UniProt ID: Q13495
• Cytogenetics: Xq28
• Summary: This gene encodes a mastermind-like domain containing protein. This protein may function as a transcriptional co-activator. Mutations in this gene are the cause of X-linked hypospadias type 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]; Transcript Variant: This variant (2) has a shorter 5' UTR and has multiple differences in the coding region, compared to variant 1, one of which results in a translational frameshift. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1.