Pirh2 (RCHY1) (NM_015436) Human Untagged Clone

SKU SC107968

RCHY1 (untagged)-Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Pirh2
• Synonyms: ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY; ZNF363
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107968 sequence for NM_015436 edited (data generated by NextGen Sequencing); ATGGCGGCGACGGCCCGGGAAGATGGCGCCAGCGGTCAAGAGCGAGGTCAGCGGGGCTGC; GAGCACTATGACAGAGGATGTCTCCTAAAGGCACCTTGCTGTGACAAGCTTTATACTTGC; CGCTTGTGTCATGATAACAATGAAGATCATCAACTAGATCGCTTTAAAGTGAAGGAAGTG; CAGTGCATAAACTGTGAAAAAATTCAACATGCCCAACAGACTTGTGAAGAATGTAGCACA; TTGTTTGGAGAATATTATTGCGATATATGCCATTTGTTTGACAAAGATAAGAAGCAGTAT; CACTGTGAAAACTGTGGAATTTGTAGGATTGGTCCAAAGGAAGATTTTTTCCATTGTTTG; AAATGTAACTTATGCCTAGCTATGAATCTTCAAGGAAGACACAAGTGTATTGAAAATGTG; TCCCGACAGAATTGTCCAATATGTTTGGAGGACATTCACACATCCCGTGTTGTTGCTCAT; GTCTTGCCATGTGGACATCTTTTACATAGAACGTGTTATGAAGAAATGTTGAAAGAAGGC; TACAGATGTCCATTATGTATGCACTCTGCTTTAGATATGACCAGGTATTGGAGACAGCTG; GATGATGAAGTAGCACAGACTCCTATGCCATCAGAATATCAGAACATGACTGTGGATATT; CTCTGCAATGACTGTAATGGACGATCCACTGTTCAGTTTCATATATTAGGCATGAAATGT; AAGATTTGTGAATCCTATAATACTGCTCAAGCTGGAGGACGTAGAATTTCACTGGATCAG; CAATGA; ; Clone variation with respect to NM_015436.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015436 unedited; GCCGCTGAATTCGCACGAGGGTGGAATGTTATGGGAGGTGGAGGAGATGGCGGCGACGGC; CCGGGAAGATGGCGCCAGCGGTCAAGAGCGAGGTCAGCGGGGCTGCGAGCACTATGACAG; AGGATGTCTCCTAAAGGCACCTTGCTGTGACAAGCTTTATACTTGCCGCTTGTGTCATGA; TAACAATGAAGATCATCAACTAGATCGCTTTAAAGTGAAGGAAGTGCAGTGCATAAACTG; TGAAAAAAATTCAACATGCCCAACAGACTTGTGAAGAATGTAGCACATTGTTTGGAGAAT; ATTATTGCGATATATGCCATTTGTTTGACAAAGATAAGAAGCAGTATCACTGTGAAAACT; GTGGAATTTGTAGGATTGGTCCAAAGGAAGATTTTTTCCATTGTTTGAAATGTAACTTAT; GCCTAGCTATGAATCTTCAAGGAAGACACAAGTGTATTGAAAATGTGTCCCGACAGAATT; GTCCAATATGTTTGGAGGACATTCACACATCCCGTGTTGTTGCTCATGTCTTGCCATGTG; GACATCTTTTACATAGAACGTGTTATGAAGAAATGTTGAAAGAAGGCTACAGATGTCCAT; TATGTATGCACTCTGCTTTAGATATGACCAGGTATTGGAGACAGCTGGATGATGAAGTAG; CACAGACTCCTATGCCATCAGAATATCAGAACATGACTGTGGATATTCTCTGCAATGAAC; TGTATGGACGATCCACTGTTCAGTTTCATATATTANGCATGAAAATGTAAGATTTGTGAA; TTCTATTATACTGCTCAAGCTTGGAGACCTAGAATTTCACTGGATCAGCAATGACGACCC; TCACAGTACTGGAGAACTTGCAATTTCTGATTTATAAAAAGCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015436 unedited; TCACCTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTGGTTTT; TTTACCACAAAAAAGGTATGAGGCAATAAATTTATTACATTGATTTAATCATTCTACACT; GTAAACATATATCAAAACATCACATTGTACTCCATATATATAATATACACAATTATCAAT; TAAATTTTAATAACACAAAGGACAAAAGAAAAATAAAGTGGCATAGTTTTGAACAACTCC; CATTTAAAATTAATTCATTTCAAATTAAACTATTCAACTAGTGAATACCTATGGCCAGGC; ACTTATAAAATAATGTTGGCTCTTTAGTACTTAGTAAATTTATTTTGATGCATGACCAAA; ATAAAAGGGAAATTTAACATGTATATCATATGAAAATAGGTTTTAGTGAAGAAATAAAAA; CTGAGTTTGATTTTAAAATCCATGACACTATAAACAAACTACTACTTCAAACATAAGCTA; AAGTTAATTCATAAAGAGTTCTATGTACTGGTTAAGAAGACCCCAGAAACTAAGAAATGA; ACCACTTGGTATACACTCATTTTTTTTTTATACATACATCTTTAATGATAAAAGAAACTG; AGATGCTATGCACCAAAAAATTCCTAAGGGGATTGGTATTTTACCTTTTTTAAAAATAAT; CCCAATAAACTATAGATACAAATTCTTGCGCAGGGCAACCCTACTTATTTCAGGGACAGA; ATATTCTAGTGTACTTAAGAACTGTCTAAGTAATTAACAATCCCTGGAGCCTTTTAAAAA; GAATAGGAAAAGAAGAAGATGTCTGTTAAAATGGTAACAGTAGTTCTCTCTGAAAGAAGG; NATGTTGAGAGATTTTTACCTTCTTCTTTTTGNAGGATAAACTTTCCTATTGAGTCCGAA; AAGGAAAAAAATTANGGTAAAGTTTTGATTTTAATTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_015436
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015436.2, NP_056251.2
• RefSeq Size: 4336 bp
• RefSeq ORF: 786 bp
• Locus ID: 25898
• UniProt ID: Q96PM5
• Cytogenetics: 4q21.1
• Domains: RING
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: p53 signaling pathway, Ubiquitin mediated proteolysis
• Summary: The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013]; Transcript Variant: This variant (1) encodes the longest isoform (1, also known as Pirh2A).