CD82 (NM_002231) Human Untagged Clone

CAT#: SC107926

CD82 (untagged)-Human CD82 molecule (CD82), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD82
• Synonyms: 4F9; C33; GR15; IA4; KAI1; R2; SAR2; ST6; TSPAN27
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107926 sequence for NM_002231 edited (data generated by NextGen Sequencing)
  ATGGGCTCAGCCTGTATCAAAGTCACCAAATACTTTCTCTTCCTCTTCAACTTGATCTTC
  TTTATCCTGGGCGCAGTGATCCTGGGCTTCGGGGTGTGGATCCTGGCCGACAAGAGCAGT
  TTCATCTCTGTCCTGCAAACCTCCTCCAGCTCGCTTAGGATGGGGGCCTATGTCTTCATC
  GGCGTGGGGGCAGTCACTATGCTCATGGGCTTCCTGGGCTGCATCGGCGCCGTCAACGAG
  GTCCGCTGCCTGCTGGGGCTGTACTTTGCTTTCCTGCTCCTGATCCTCATTGCCCAGGTG
  ACGGCCGGGGCCCTCTTCTACTTCAACATGGGCAAGCTGAAGCAGGAGATGGGCGGCATC
  GTGACTGAGCTCATTCGAGACTACAACAGCAGTCGCGAGGACAGCCTGCAGGATGCCTGG
  GACTACGTGCAGGCTCAGGTGAAGTGCTGCGGCTGGGTCAGCTTCTACAACTGGACAGAC
  AACGCTGAGCTCATGAATCGCCCTGAGGTCACCTACCCCTGTTCCTGCGAAGTCAAGGGG
  GAAGAGGACAACAGCCTTTCTGTGAGGAAGGGCTTCTGCGAGGCCCCCGGCAACAGGACC
  CAGAGTGGCAACCACCCTGAGGACTGGCCTGTGTACCAGGAGGGCTGCATGGAGAAGGTG
  CAGGCGTGGCTGCAGGAGAACCTGGGCATCATCCTCGGCGTGGGCGTGGGTGTGGCCATC
  ATCGAGCTCCTGGGGATGGTCCTGTCCATCTGCTTGTGCCGGCACGTCCATTCCGAAGAC
  TACAGCAAGGTCCCCAAGTACTGA
  
  Clone variation with respect to NM_002231.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002231 unedited
  GCAGCCCAATTCGCACGAGGCCGACGCCGTCTGAGGCACGAGCGGGTGACGCTGGGCCTG
  CAGCGCGGAGCAGAAAGCAGAACCCGCAGAGTCCTCCCTGCTGCTGTGTGGACGACACGT
  GGGCACAGGCAGAAGTGGGCCCTGTGACCAGCTGCACTGGTTTCGTGGAAGGAAGCTCCA
  TACTGGCGGGATGGGCTCAGCCTGTATCAAAGTCACCAAATACTTTCTCTTCCTCTTCAA
  CTTGATCTTCTTTATCCTGGGCGCAGTGATCCTGGGCTTCGGGGTGTGGATCCTGGCCGA
  CAAGAGCAGTTTCATCTCTGTCCTGCAAACCTCCTCCAGCTCGCTTAGGATGGGGGCCTA
  TGTCTTCATCGGCGTGGGGGCAGTCACTATGCTCATGGGCTTCCTGGGCTGCATCGGCGC
  CGTCAACGAGGTCCGCTGCCTGCTGGGGCTGTACTTTGCTTTCCTGCTCCTGATCCTCAT
  TGCCCAGGTGACGGCCGGGGCCCTCTTCTACTTCAACATGGGCAAGCTGAAGCAGGAGAT
  GGGCGGCATCGTGACTGAGCTCATTCGAGACTACAACAGCAGTCGCGAGGACAGCCTGCA
  GGATGCCTGGGACTACGTGCAGGCTCAGGTGAAGTGCTGCGGCTGGGTCAGCTTCTACAA
  CTGGACAGACAACGCTGAGCTCATGAATCGCCCTGAGGTCACCTACCCCTGTTCCTGCGA
  AGTCAAGGGGGAAGAGGACAACAGCCTTTCTGTGAGGAAGGGCTTCTGCGAGGCCCCCGG
  CCACAGGACCCANAGTGGCAACCACCCTGAGGACTGGCCTGTGTACCAGGAAGGCTGCAT
  GGGAAAAGTGCAAGCTTGGCTGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002231 unedited
  GCTCTTAGCGGGCCCCTAGTCTGGAAGTGCAGTAGCACGATCTCAGCTCGCTGCAACCTT
  CTCTCCCGGGTTCACACCATTGTGCTGCCTGAGCCTCCCAATAGCTGGGACTACAGGTGC
  CCGCCACCGCGCCCGGCTAAATTTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGT
  TAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAAAGGG
  CGGGGAGTACAGGCGTGAGCCACCGCGCCCGGCCCCCTCCTCCCCAATTTTTCATACAGC
  TGCCCCTATACAATATACACACCCTTGAGGGCAGGTAGAAGTCCAGCCCACCTGCGCCAG
  GGACGCTGTGGGGAGCATTTTTCTCTGCGTTGCCTAAGAGAACCCTGATGGGCGGTGAGC
  TGAGGAACCACACAACAGTCACGGCTCAATGCTGGTACCGCCTACGCCAGGAGAGATCGT
  TATGTCCTACAAAGCCCTCTACTACCATTTAGGGAGGGCTCCAGGGTCTTCCCACTGCAC
  CGGGATGCACGCCCTGCAGACGGAGCCATGGGACATCTCTGGAGCCCCGAGGTTGTGTGT
  CACGCCTGCACATGCCATTTTCCCTTCCTCACTTCTTGTTGTTTTTTTTTTGCTTTCCCG
  ATGGCCCTCCTCCGCCTATTCGTTTGTCCGGTACTATCCCCCCTTTTTCCTTTTTCGCCC
  CTCTCTTTCCTCTCCCTCGCTCCTACTTTGCCCCCCCCGCTCCACCCGTTTTTTCTTTTC
  CTCTTCCTCTTTCGGTTCCTTATTTCCGCACTCACGTCCTTCCCTCTCCCTATCCACTTC
  TCCCGTCCCCTCTCTTTTCTCTCCCCCATTTATTCTCCCCCCTATTTTTTCCTTTTTCGC
  GGCCTCGCCTTTCCTCCCGCCTTTTTCTCTCTTTCACCGCTTTTCATCTCTCTNCTCTTA
  TCCTCTTTCTCCACGTCATTCCTACCCACCCACACCACTCTATTTCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002231
• Insert Size: 1680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002231.3, NP_002222.1
• RefSeq Size: 1715 bp
• RefSeq ORF: 804 bp
• Locus ID: 3732
• UniProt ID: P27701
• Cytogenetics: 11p11.2
• Domains: transmembrane4
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: p53 signaling pathway
• Gene Summary: This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).