AKAP associated Sperm Protein (ROPN1L) (NM_031916) Human Untagged Clone

SKU SC106880

ROPN1L (untagged)-Human rhophilin associated tail protein 1-like (ROPN1L), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: AKAP associated Sperm Protein
• Synonyms: ASP; RSPH11
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031916, the custom clone sequence may differ by one or more nucleotides; ; ; ATGCCGCTTCCCGACACCATGTTCTGCGCTCAGCAGATCCACATTCCCCCGGAGCTGCCGGACATCCTGA; AGCAATTCACCAAGGCTGCCATCCGCACCCAGCCGGCCGACGTGCTGCGGTGGTCCGCGGGCTATTTTTC; AGCTCTGTCGAGAGGAGATCCACTTCCTGTAAAGGACAGAATGGAAATGCCCACGGCAACCCAGAAAACA; GACACAGGCCTGACTCAAGGACTCCTGAAAGTTTTGCACAAGCAGTGTCACCACAAGCGGTATGTGGAAT; TAACAGATCTTGAGCAGAAGTGGAAGAACTTGTGCCTGCCGAAGGAAAAATTCAAAGCGCTCTTACAACT; GGATCCTTGTGAAAACAAAATCAAGTGGATAAACTTTTTAGCGCTTGGATGCAGCATGCTTGGTGGGTCC; TTGAACACTGCGCTGAAGCACCTGTGCGAGATCCTCACGGACGATCCGGAGGGCGGGCCCGCTCGCATCC; CCTTCAAGACGTTTTCCTACGTTTACCGCTACTTGGCCAGATTAGACTCAGATGTGTCTCCCTTGGAGAC; GGAATCCTACCTTGCCTCTCTAAAGGAAAATATAGACGCCAGGAAGAACGGCATGATAGGTCTTTCAGAT; TTCTTCTTTCCAAAGAGGAAACTTTTAGAAAGCATTGAAAACTCTGAAGATGTAGGCCATTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031916 unedited; ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGGAGGCGGCCCT; GGCCGCAAGCCCCGCGCTGCTAGCGGGTCCACCGCGTCGTAGCCGACAGCCGCCCTTCTT; CCTCGCAGCGCGCCGCGATTCACCAGCCTGGTCCCTTCTGCGGAGAGCGATGCCGCTTCC; CGACACCATGTTCTGCGCTCAGCAGATCCACATTCCCCCGGAGCTGCCGGACATCCTGAA; GCAATTCACCAAGGCTGCCATCCGCACCCAGCCGGCCGACGTGCTGCGGTGGTCCGCGGG; CTATTTTTCAGCTCTGTCGAGAGGAGATCCACTTCCTGTAAAGGACAGAATGGAAATGCC; CACGGCAACCCAGAAAACAGACACAGGCCTGACTCAAGGACTCCTGAAAGTTTTGCACAA; GCAGTGTCACCACAAGCGGTATGTGGAATTAACAGATCTTGAGCAGAAGTGGAAGAACTT; GTGCCTGCCGAAGGAAAAATTCAAAGCGCTCTTACAACTGGATCCTTGTGAAAACAAAAT; CAAGTGGATAAACTTTTTAGCGCTTGGATGCAGCATGCTTGGTGGGTCCTTGAACACTGC; GCTGAAGCACCTGTGCGAGATCCTCACGGACGATCCGGAGGGCGGGCCCGCTCGCATCCC; CTTCAAGACGTTTTCCTACGTTTACCGCTACTTGGCCAGATTAGACTCAGATGTGTCTCC; CTTGGAGACGGNATCCTACCTTGCCTCNTCTAAGGAAATATAGACGCCAGNAAGAAACGC; ATGATAGGTCTTTCAGATTTCTTCTTTCCAAGAGGNAACTNTTAGAAGCATTGAAACTCT; GNAGATGTAGGCCATTATACAGAGAAGATACATTNTC;
• Restriction Sites: Please inquire
• ACCN: NM_031916
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_031916.2, NP_114122.2
• RefSeq Size: 1052 bp
• RefSeq ORF: 693 bp
• Locus ID: 83853
• UniProt ID: Q96C74
• Cytogenetics: 5p15.2
• Summary: This gene encodes a member of the ropporin family. The encoded protein is present in sperm and interacts with A-kinase anchoring protein, AKAP3, through the amphipathic helix region of AKAP3. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2014]; Transcript Variant: This variant (1) and variant 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.