KCTD10 (NM_031954) Human Untagged Clone

SKU
SC106747
KCTD10 (untagged)-Human potassium channel tetramerisation domain containing 10 (KCTD10)
$300.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol KCTD10
Synonyms BTBD28; hBACURD3; MSTP028; ULRO61
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC106747 sequence for NM_031954 edited (data generated by NextGen Sequencing)
ATGGAAGAGATGTCAGGAGAAAGTGTGGTGAGCTCAGCGGTGCCAGCGGCTGCTACCCGC
ACCACTTCCTTCAAGGGCACGAGCCCCAGCTCCAAATACGTGAAGCTGAATGTGGGTGGA
GCCCTCTACTATACCACCATGCAGACGCTGACCAAGCAGGACACCATGCTGAAGGCCATG
TTCAGCGGGCGCATGGAAGTGCTCACCGACAGTGAAGGCTGGATCCTCATTGACCGCTGT
GGGAAGCACTTTGGTACGATACTCAACTACCTTCGAGACGGGGCGGTGCCTTTACCCGAG
AGCCGCCGGGAGATCGAGGAGCTGCTAGCAGAAGCCAAGTACTACCTAGTCCAAGGCCTG
GTGGAAGAGTGCCAGGCGGCCCTACAAAACAAAGATACTTATGAGCCTTTCTGCAAGGTC
CCTGTGATCACCTCATCCAAGGAAGAACAAAAACTTATAGCGACTTCAAATAAGCCAGCC
GTGAAGTTGCTCTACAACAGAAGTAACAACAAATACTCATATACCAGCAATTCTGACGAC
AATATGTTGAAAAACATTGAACTGTTTGATAAGCTGTCTCTGCGCTTTAACGGAAGGGTC
CTGTTCATAAAGGATGTTATTGGGGATGAAATCTGCTGCTGGTCCTTTTATGGTCAGGGC
CGGAAGATTGCTGAAGTCTGTTGTACCTCCATCGTCTATGCCACTGAGAAGAAACAGACC
AAGGTGGAGTTTCCCGAAGCCCGGATTTATGAGGAGACCCTGAACATTTTGCTGTATGAG
GCCCAGGATGGCCGGGGACCTGACAATGCGCTCCTGGAGGCCACAGGCGGGGCGGCGGGG
CGCTCCCACCACCTGGACGAGGACGAGGAGCGGGAGCGGATCGAGCGCGTGCGGAGGATC
CACATCAAGCGCCCTGATGACCGGGCCCACCTCCACCAGTGA

Clone variation with respect to NM_031954.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_031954 unedited
ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCTGCGTCCTC
CGACTTTTCATGGAAGAGATGTCAGGAGAAAGTGTGGTGAGCTCAGCGGTGCCAGCGGCT
GCTACCCGCACCACTTCCTTCAAGGGCACGAGCCCCAGCTCCAAATACGTGAAGCTGAAT
GTGGGTGGAGCCCTCTACTATACCACCATGCAGACGCTGACCAAGCAGGACACCATGCTG
AAGGCCATGTTCAGCGGGCGCATGGAAGTGCTCACCGACAGTGAAGGCTGGATCCTCATT
GACCGCTGTGGGAAGCACTTTGGTACGATACTCAACTACCTTCGAGACGGGGCGGTGCCT
TTACCCGAGAGCCGCCGGGAGATCGAGGAGCTGCTAGCAGAAGCCAAGTACTACCTAGTC
CAAGGCCTGGTGGAAGAGTGCCAGGCGGCCCTACAAAACAAAGATACTTATGAGCCTTTC
TGCAAGGTCCCTGTGATCACCTCATCCAAGGAAGAACAAAAACTTATAGCGACTTCAAAT
AAGCCAGCCGTGAAGTTGCTCTACAACAGAAGTAACAACAAATACTCATATACCAGCAAT
TCTGACGACAATATGTTGAAAAACATTGAACTGTTTGATAAGCTGTCTCTGCGCTTTAAC
GGAAGGGTCCTGTTCATAAAGGATGTTATTGGGGATGAAATCTGCTGCTGGTCCTTTTAT
GGTCAGGNCCGNAAGAATGCTGAAGTCTGTTGTACCTCCATCGTCTATGCCACTGAGAAG
AAACAGACCAAGGTGGAGTTTTCCGAAGCCCGTATTATGAGGAGACCCCTGACATTTTGC
TGTATGAAGCCCCAGATGGGCCGGGACCTGACATGCGCTCCTGGAGCCACAGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_031954 unedited
TATGGAACGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATTGGTTTAAA
GTTTTATTGTAGACTTTGCTGTTGGATACAAAATGAAGGCATACAACTGTCACAGGCAGG
GCAGTAAGTACAAAGTCTAAGCTGTAAAAACCGTTTGAAAATATAAACTCGTTTTTGGAA
TACATGTGTCAAAGGCTGCCCATGTTAATACCTTTGGTATAAAACGGTAACGATTCCCTT
GACAAACCCATCCATCACCTGACGCACATTCACATCTCCTGGTAACTACTCTACCTAGTC
TAGTCTCAACCACCCCTGTCAGTCACGACTCACTCCTGTTCCTTTGCAGGTGCAGAGGAG
CCTGGGAGGTAGGTCACTGAGAACACCCGTCCTACATAGGCGCTGCGCCCAGCCGGGCTC
CAGCAGGGGCCACCTGTGCCCCACAAGCACACGTCCCCACCCACAGTCACACATATCCCT
TAAACAAACATGAACACAGACACAAACCAGACCCAAAGTTATCTATCTACCCGCACATCC
TCAACCTGTTCACTGCTGGCCACTCTACACTGCGCAAAAATCAAAGGCCAAGACAAGGGG
GCCTGTGCCCATAGCATGGAAGCTCCCTGTGGCCCGTGGCGTTAGGGTTTTCCTTGCTGT
ATCCCCCCTGGGATGCCAATGGTTTGGGCCACCCGCATTTTTCCTCAACTGTCTTCTCCC
GTTTCCCCCCTTCTTTCCCCCGCCCCCCCAATGATTTGACCCCCCGTTGCCCACCCCCCC
CCCTTCCCGCTCCATGCCCAAATTCCTTCCTGTACCGCACCCGCGCCCCCCCCCCCCATT
TTCACCCCCCTCTCCCCGCTCAATTCCTCTTCCTGATCGGCCACCCCTTCCGCGCCTTGC
CCCAGCACACAGATCGTGGTGTACCCATTATTATTTTCGTTCATGTTTTTGTTTTCTCCC
CACTTTCTT
Restriction Sites NotI-NotI
ACCN NM_031954
Insert Size 2660 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031954.3, NP_114160.1
RefSeq Size 4057 bp
RefSeq ORF 942 bp
Locus ID 83892
UniProt ID Q9H3F6
Cytogenetics 12q24.11
Domains BTB, K_tetra
Protein Families Ion Channels: Other
Summary The protein encoded by this gene binds proliferating cell nuclear antigen (PCNA) and may be involved in DNA synthesis and cell proliferation. In addition, the encoded protein may be a tumor suppressor. Several protein-coding and non-protein coding transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]
Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1.
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Citations

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