PCGF5 (NM_032373) Human Untagged Clone

SKU SC105003

PCGF5 (untagged)-Human polycomb group ring finger 5 (PCGF5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PCGF5
• Synonyms: RNF159
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032373, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCTACCCAAAGGAAACACTTGGTGAAAGATTTTAATCCTTACATTACCTGCTATATCTGTAAAGGGT; ATCTGATCAAGCCAACAACAGTGACGGAATGCCTCCATACATTCTGTAAGACTTGTATTGTTCAGCACTT; TGAAGATAGCAATGATTGCCCAAGGTGTGGCAACCAAGTTCATGAGACAAATCCATTAGAAATGTTGAGG; TTGGACAATACATTAGAGGAAATTATATTTAAGCTGGTCCCTGGACTACGAGAACAAGAACTTGAGCGTG; AATCTGAATTTTGGAAGAAAAATAAGCCTCAAGAAAATGGACAAGATGATACTTCAAAAGCTGACAAACC; GAAAGTAGATGAAGAAGGTGATGAAAATGAAGATGATAAAGATTATCACAGAAGTGACCCACAAATTGCT; ATCTGTCTAGATTGTTTACGAAATAATGGGCAATCAGGGGACAATGTAGTAAAGGGTTTAATGAAGAAAT; TCATTCGATGTTCTACACGTGTAACTGTGGGAACTATTAAAAAATTTCTAAGTTTAAAACTAAAACTTCC; AAGTTCTTATGAGTTGGATGTGCTGTGCAATGGTGAAATTATGGGGAAGGATCATACTATGGAATTCATC; TACATGACAAGATGGCGACTAAGAGGCGAAAACTTTCGGTGTCTGAACTGCTCAGCTTCGCAAGTCTGCT; CTCAGGATGGCCCTTTGTATCAGTCATACCCTATGGTACTGCAGTATCGACCAAGAATTGATTTCGGTTA; G; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032373 unedited; NGGTGTCAGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCTGGCG; GGCGAGGAGCGGCGGCGGTGGCGGCCGCTGGACATGGGAAAGCGGAACCACCAAAAGGAG; TGATGATCAACGATCTCATGATAAATCTGGATGCTAGTTCTCATGCCTCAGGACATCCTA; CTGGGAACGACACACCAGCTCCTGGGATCAGACTTTCATCTACTTAGGACCCCTCTTTGC; CCAGACTACTAAAGCCAGTCTTCACTAGCCACGAATGGCTACCCAAAGGAAACACTTGGT; GAAAGATTTTAATCCTTACATTACCTGCTATATCTGTAAAGGGTATCTGATCAAGCCAAC; AACAGTGACGGAATGCCTCCATACATTCTGTAAGACTTGTATTGTTCAGCACTTTGAAGA; TAGCAATGATTGCCCAAGGTGTGGCAACCAAGTTCATGAGACAAATCCATTAGAAATGTT; GAGGTTGGACAATACATTAGAGGAAATTATATTTAAGCTGGTCCCTGGACTACGAGAACA; AGAACTTGAGCGTGAATCTGAATTTTGGAAGAAAAATAAGCCTCAAGAAAATGGACAAGA; TGATACTTCAAAAGCTGACAAACCGAAAGTAGATGAAGAAGGTGATGAAAATGAAGATGA; TAAAGATTATCACAGAAGTGACCCACAAATTGCTATCTGTCTAGATTGTTTACGAAATAA; TGGGCAATCAGGGGACAATGTAGTAAAGGGTTTAATGAAGAAATTCATTCGATGTTCTAN; CACGTGTACTGTGGGAACTATTAAAAATTTCTAAGTTTAAACTAAACTTNCAGTTCTTAT; GAGTTGGATGTGCTGTGCATGGTGAATTATGGGGAAGGATCATCTATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_032373
• Insert Size: 6610 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032373.2, NP_115749.2
• RefSeq Size: 7222 bp
• RefSeq ORF: 771 bp
• Locus ID: 84333
• UniProt ID: Q86SE9
• Cytogenetics: 10q23.32
• Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:26151332). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332). Plays a redundant role with PCGF3 as part of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 4 all encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.