Cofilin 2 (CFL2) (NM_021914) Human Untagged Clone

SKU SC102370

CFL2 (untagged)-Human cofilin 2 (muscle) (CFL2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cofilin 2
• Synonyms: NEM7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021914, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCTTCTGGAGTTACAGTGAATGATGAAGTCATCAAAGTTTTTAATGATATGAAAGTAAGGAAATCTT; CTACACAAGAGGAGATCAAAAAGAGAAAGAAAGCAGTTCTCTTCTGTTTAAGCGATGACAAAAGACAAAT; AATTGTAGAGGAAGCAAAGCAGATCTTGGTGGGTGACATTGGTGATACTGTAGAGGACCCCTACACATCT; TTTGTGAAGTTGCTACCTCTGAATGATTGCCGATATGCTTTGTACGATGCCACATACGAAACAAAAGAGT; CTAAGAAAGAAGACCTAGTATTTATATTCTGGGCTCCTGAAAGTGCACCTTTAAAAAGCAAGATGATTTA; TGCTAGCTCTAAAGATGCCATTAAAAAGAAATTTACAGGTATTAAACATGAGTGGCAAGTAAATGGCTTG; GATGATATTAAGGACCGTTCGACACTTGGAGAGAAATTGGGAGGCAATGTAGTAGTTTCACTTGAAGGAA; AACCATTATAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021914 unedited; GCACGAGGCCGGCGTGCCCCGCACTCTCCGCTGCCCACCCGCTCGCCCGCCCCTCCTTCT; CCTCCCAGTGCCACAGAGCCGAAGCCCGAGCTGCCGCCGCAGCCACAGCCGAGGGCACTA; TGGCTTCTGGAGTTACAGTGAATGATGAAGTCATCAAAGTTTTTAATGATATGAAAGTAA; GGAAATCTTCTACACAAGAGGAGATCAAAAAGAGAAAGAAAGCAGTTCTCTTCTGTTTAA; GCGATGACAAAAGACAAATAATTGTAGAGGAAGCAAAGCAGATCTTGGTGGGTGACATTG; GTGATACTGTAGAGGACCCCTACACATCTTTTGTGAAGTTGCTACCTCTGAATGATTGCC; GATATGCTTTGTACGATGCCACATACGAAACAAAAGAGTCTAAGAAAGAAGACCTAGTAT; TTATATTCTGGGCTCCTGAAAGTGCACCTTTAAAAAGCAAGATGATTTATGCTAGCTCTA; AAGATGCCATTAAAAAGAAATTTACAGGTATTAAACATGAGTGGCAAGTAAATGGCTTGG; ATGATATTAAGGACCGTTCGACACTTGGAGAGAAATTGGGAGGCAATGTAGTAGTTTCAC; TTGAAGGAAAACCATTATAAAATG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021914 unedited; ATTTTTATTGCAACGTGGCCCTTTTTGTGAGGGTGGGGAGTTTGATCTCAAAACAATGTT; CCATTTAAGGCTCTTTTATACAGAAATTGCCATCATGACTGATATTCAAAATATCTTTAG; TGTTGCAGGACTCACATGGTAAACATAAAACTCCTACACTTATTCAGTAGTGTACACTCA; ATGGAAAACAAAAAGGCATTAATAACAGCTATTTCTTTTAAGAAGATATGCAGGTAACAG; GAATGAACACTGAGGTACTAGGATAAGTTGATGACACAGTTAACAAAACTTAATTGGCAT; TCCTTTTAGGATATTAAACTTATTACAAAAAGTGCTTTTAATGCATAGTGTTATATCCGT; GCTGCCATATCACTAAAATAGGCTTGCCAAGGCAGGTGAGGTGTATGAATGCTCAAGCCT; CACAGAACTGCAATCAAGTGCCAACTATAAATAATACTGAAAAAAGTTGACCATCTGACC; AGTGGATAATACTTTCAAGGCATTCAATTAGCTTATCCTTTGCAGTATTCTAAGCTATTC; ACATTGACGATCACATACATTGTAGTGCTTGCTGCAAGGGAGGCATATAACCAGTTGTTT; TGGCTAAAATATGACAGGAAGGCATTCCTTGGGTTCTATATAAAAGTAACAGTATTCAAC; AGTCTAATGGCAATCACTGATAGGCTGCTTAAATAATGATATTTCCTTCATTCATTNGTG; TGGAAGGGCCCAGTGGAAAGGNNGGAAATACACANAAANCCAAACCCTATACTATTNCCA; TGGACTGAGCTGGAGAATGAAAGCTCTTNNAGACCAGATGGCACTNGACTGTCATTTATA; ATGGNTTTCCTCAGTGAAACTCTACATTGNCTNCCAATTCTCTNCAGTGTCGAACGGTCC; TAATTCATNNCAGCCATTTACTGCACTATGTTAATACCTGAANTNCTTNTATGGCATCTT; AGAGCTACTAATATCTGCTTTTAAGGTGCCTTCAGAGCCGATATATACTAGGCTCTTCTA; AACTTTTTGTTCNTGGGCATGCCAAGCTTCGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_021914
• Insert Size: 1540 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021914.5, NP_068733.1
• RefSeq Size: 3093 bp
• RefSeq ORF: 501 bp
• Locus ID: 1073
• UniProt ID: Q9Y281
• Cytogenetics: 14q13.1
• Domains: ADF
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Summary: This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]; Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate translational start codon, compared to variant 2, but the encoded isoform (1) is identical to that encoded by variant 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.