Wls (NM_001085353) Rat Untagged Clone

SKU RN216879

Wls (untagged) - Rat wntless Wnt ligand secretion mediator (Wls), transcript variant 2

Product Data

• Type: Rat Untagged Clone
• Target Symbol: Wls
• Synonyms: Gpr177
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216879 representing NM_001085353; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGGGGCAATTATAGAAAACATGAGCACCAAGAAGCTCTGCATTGTTGGAGGGATTCTTCTGGTTT; TCCAAATCGTTGCCTTTCTGGTGGGAGGCTTGATCGCTCCAGCACCCACAACGGCAGTGTCCTACGTGGC; AGCAAAATGTGTGGATGTCCGGAAGAACCACCATAAAACAAGATGGCTGATGCCCTGGGGACCAAACAAG; TGTAACAAGATCAATGACTTCGAAGAAGCAATTCCAAGGGAAATTGAAGCGAATGACATTGTGTTTTCTG; TACACATTCCCCTCCCTTCTATGGAGATGAGCCCATGGTTCCAGTTTATGCTGTTTATCCTGCAGATAGA; CATTGCTTTCAAGCTAAACAACCAAATCAGAGAAAATGCAGAAGTTTCCATGGATGTTTCCCTGGGTTAC; CGTGATGATATGTTTTCTGAGTGGACTGAAATGGCGCACGAAAGAGTACCACGTAAACTCAGATGCACTT; TCACATCCCCCAAGACCCCAGAGCATGAAGGTCGTCATTATGAATGTGATGTCCTTCCTTTCATGGAAAT; TGGGTCAGTGGCTCATAAGTATTACCTTCTAAATATCCGGCTACCTGTAAATGAGAAGAAGAAAATCAAT; GTTGGAATTGGGGAAATAAAGGACATTCGGTTGGTGGGAATCCACCAAAATGGAGGTTTCACTAAGGTAT; GGTTTGCTATGAAGACCTTCCTCACACCCAGCATCTTCATCATTATGGTGTGGTATTGGAGAAGGATCAC; CATGATGTCCCGACCTCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATC; AATATCCCTGTGGAATGGTTTTCCATTGGATTTGATTGGACCTGGATGCTGTTATTTGGTGACATACGAC; AGGGCATCTTCTATGCAATGCTTCTTTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCAACA; TGAGCGGAATCACATTGCAGGGTATTGGAAGCAAGTTGGACCAATTGCTGTTGGCTCTTTCTGCCTCTTC; ATATTTGACATGTGTGAGAGAGGAGTGCAACTCACAAATCCTTTCTACAGTATCTGGACTACAGATGTTG; GAACAGAACTGGCTATGGCTTTCATCATTGTGGCAGGTATCTGCCTCTGCCTCTACTTCCTGTTTCTGTG; TTTCATGGTATTTCAAGTATTCAGAAACATCAGTGGGAAACAGTCTAGCCTCCCAGCCATGAGCAAAGTC; CGGAGGCTGCACTATGAGGGTCTGATTTTCAGGTTCAAGTTCCTCATGCTGATCACCTTGGCTTGTGCTG; CCATGACTGTTATCTTCTTCATTGTTAGTCAGGTGACAGAAGGCCATTGGAAATGGGGTGGGGTCACAGT; TCAAGTGAGCAGTGCTTTCTTCACTGGAATCTATGGGATGTGGAACCTGTATGTCTTTGCTTTGATGTTC; TTGTATGCACCATCCCATAAGAACTATGGGGAAGACCAGTCTAATGGTGACCTGGGTGTCCACAGCGGGG; AAGAACTGCAGCTCACTACCACAATCACCCATGTAGATGGACCGACTGAGATCTACAAGTTGACCCGTAA; AGAAGCACAGGAGTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001085353
• Insert Size: 1626 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001085353.1, NP_001078822.1
• RefSeq Size: 2640 bp
• RefSeq ORF: 1626 bp
• Locus ID: 362065
• UniProt ID: Q6P689
• Cytogenetics: 2q45
• Summary: Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 encode the same protein.