Gpbar1 (NM_177936) Rat Untagged Clone

SKU RN210451

Gpbar1 (untagged ORF) - Rat G protein-coupled bile acid receptor 1 (Gpbar1), (10 ug)

Product Data

• Type: Rat Untagged Clone
• Target Symbol: Gpbar1
• Synonyms: Tgr5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210451 representing NM_177936; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATGTCACACAACACCACTGAGCTGTCAGCCATTCCCAGAGGGGTTCAGGAGCTTTCCCTGGTCCTGG; CAAGCCTCATCGTCATCGCCAACCTGCTCCTGGCCCTAGGCATTGTCCTGGACCGCCACTTACGCAGCCC; ACCTGCTGGCTGCTTCTTTCTAAGCCTACTACTAGCTGGGCTACTCACAGGGTTGGCACTGCCCACGCTG; CCTGGGCTATGGAATAGGAGCCATCAGGGGTACTGGTCCTGCCTCCTTCTCCACTTGGCCCCCAACTTTT; GTTTCCTCTCCCTGCTTGCCAATCTGCTGCTGGTGCATGGGGAACGCTACATGGCAGTGTTGCAGCCACT; CCGGCCCCATGGGAGTGTGCGGCTAGCCCTGTTCCTCACCTGGATCAGCTCCCTGCTCTTTGCCAGCCTG; CCTGCTCTGGGCTGGAACCACTGGAGTCCTGGTGCCAACTGCAGCTCCCAGGCTATCTTCCCAGCCCCCT; ACCTTTACCTCGAAGTCTATGGGCTCCTGCTGCCCGCTGTGGGGGCCACTGCCCTTCTCTCTGTCCGAGT; GTTGGCCACTGCCCACCACCAGCTGCGGGAGATCCGCAGACTGGAGCGGGCGGTGTGCCGTGATGCACCC; TCAACCCTAGCGAGGGCTCTCACCTGGAGGCAGGCTAGGGCACAGGCAGGAGCCACACTGCTCTTTTTGC; TGTGTTGGGGGCCCTATGTGGCCACATTGCTCCTGTCAGTCTTGGCCTATGAGCGGCGGCCACCACTAGG; GCCTGTAACTCTGTTATCTCTCATCTCATTGGGCAGTGCCAGTGCTGCAGTTGTGCCTGTGGCCATGGGT; CTGGGTGATCAGCGCTACACGGCCCCCTGGAGGACAGCTGCCCAAAGGTGGCTACAAGTGCTTCGAGGAA; GACCCAAGAGGGCCAATCCAGGCCCCAGCACTGCCTACCACTCCAGTAGCCAATGCAGCACTGACTTGGA; CTTGAATTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI
• ACCN: NM_177936
• Insert Size: 990 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_177936.1, NP_808797.1
• RefSeq Size: 990 bp
• RefSeq ORF: 990 bp
• Locus ID: 338443
• UniProt ID: Q80T02
• Cytogenetics: 9q33
• Summary: a bile acid receptor [RGD, Feb 2006]