PIK3C2G (NM_001288772) Human Tagged ORF Clone

SKU RG240163

PIK3C2G (tGFP-tagged) - Human phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 gamma (PIK3C2G), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PIK3C2G
• Synonyms: PI3K-C2-gamma; PI3K-C2GAMMA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240163 representing NM_001288772.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCATATTCTTGGCAAACGGATCCAAATCCTAATGAATCACACGAAAAGCAGTATGAACACCAAGAA; TTTCTCTTTGTAAATCAACCCCATTCTTCTAGCCAAGTCAGTCTGGGTTTTGATCAGATAGTAGATGAG; ATCAGTGGCAAAATTCCACACTACGAGAGTGAAATTGATGAAAACACCTTTTTTGTGCCCACTGCACCA; AAATGGGACTCAACAGGGCATTCATTAAATGAAGCACACCAAATATCCTTGAATGAATTCACTTCTAAA; AGCCGTGAACTCTCCTGGCATCAAGTTAGCAAAGCACCAGCAATTGGTTTTAGTCCTTCTGTGTTACCA; AAACCTCAAAATACGAATAAAGAATGCTCCTGGGGAAGCCCCATAGGAAAACATCATGGTGCTGATGAT; TCCAGATTCAGTATTTTAGCTCCATCATTCACAAGTTTGGATAAAATTAATCTAGAGAAAGAATTAGAA; AATGAAAATCATAACTACCATATAGGATTTGAAAGTAGCATTCCTCCAACAAATTCATCCTTCTCAAGT; GACTTCATGCCGAAAGAAGAGAATAAAAGGAGTGGACATGTGAACATTGTGGAACCATCTTTGATGCTT; TTGAAAGGCTCTCTTCAACCCGGAATGTGGGAAAGTACATGGCAGAAGAATATAGAGTCAATAGGTTGT; TCCATTCAGCTAGTGGAAGTACCTCAAAGCAGCAATACGAGTCTGGCCTCTTTTTGCAACAAAGTAAAA; AAAATCAGAGAAAGATATCATGCAGCTGATGTTAATTTCAATTCTGGGAAGATCTGGAGCACTACTACA; GCATTTCCGTATCAGCTCTTTTCTAAGACCAAGTTTAATATACATATTTTTATTGATAACTCAACACAA; CCTCTTCATTTTATGCCATGTGCTAATTATCTTGTCAAAGATCTAATTGCAGAAATTCTGCATTTTTGC; ACAAATGACCAGCTACTCCCCAAAGATCATATTCTAAGTGTATGTGGCTCTGAAGAATTTTTACAAAAC; GACCACTGTTTGGGGAGCCACAAAATGTTTCAAAAAGATAAATCTGTTATTCAGCTCCACCTGCAGAAA; AGTAGGGAAGCTCCAGGAAAGCTATCTCGAAAGCATGAAGAGGACCACAGTCAGTTTTATCTGAATCAA; CTTCTAGAATTTATGCATATTTGGAAAGTATCCAGACAATGTCTCTTAACACTCATCAGAAAATATGAC; TTCCACCTGAAATACCTATTGAAAACCCAGGAAAACGTGTATAATATTATTGAAGAAGTTAAAAAAATA; TGCAGTGTTCTAGGGTGTGTGGAAACCAAACAAATTACAGATGCAGTAAATGAACTAAGTCTAATTCTT; CAGAGAAAAGGAGAGAATTTTTATCAAAGTTCAGAGACTTCAGCAAAAGGCTTGATAGAGAAGGTAACA; ACTGAACTATCCACATCCATCTACCAGCTAATCAATGTCTACTGTAACAGCTTTTATGCAGATTTTCAG; CCTGTAAATGTACCTAGATGCACTTCCTATCTAAATCCCGGGCTTCCTTCCCACCTCAGCTTCACAGTG; TATGCAGCACACAACATTCCAGAAACCTGGGTGCACAGCTACAAAGCGTTTTCTTTTACCTGTTGGCTT; ACATATGCTGGAAAGAAGCTGTGCCAAGTGAGAAACTACAGAAATATTCCAGACAAGAAATTATTTTTT; TTCTTGGTCAACTGGAATGAAACGATCAATTTTCCCCTTGAAATAAAGTCACTTCCAAGGGAATCCATG; CTCACTGTAAAACTGTTTGGGATTGCCTGTGCAACCAACAATGCAAATTTACTGGCGTGGACTTGTCTT; CCACTGTTTCCAAAAGAAAAATCCATTCTCGGGTCTATGCTGTTCAGCATGACATTACAGAGTGAGCCT; CCCGTAGAAATGATAACTCCAGGAGTGTGGGATGTAAGTCAGCCATCCCCGGTGACCCTGCAGATTGAT; TTTCCAGCTACTGGGTGGGAGTATATGAAACCTGATTCTGAAGAGAATAGAAGTAATCTTGAAGAGCCA; CTAAAGGAGTGTATAAAACATATTGCCAGACTTTCACAGAAACAGACTCCCCTACTACTCTCTGAAGAA; AAGAAAAGATATTTATGGTTTTATCGCTTCTACTGCAATAATGAAAACTGCTCCCTTCCTTTAGTCCTG; GGTAGTGCCCCTGGATGGGATGAAAGGACTGTTTCAGAAATGCATACCATTTTGAGAAGATGGACATTT; TCTCAACCTTTAGAGGCTCTTGGGCTTTTGACTTCCAGTTTTCCAGATCAAGAAATTCGTAAAGTGGCA; GTTCAACAATTAGACAACCTCTTGAATGATGAACTACTGGAATATCTCCCACAGCTAGTTCAGGCTGTC; AAGTTTGAATGGAACCTTGAGAGTCCTTTAGTGCAACTTCTACTCCACCGCTCCTTGCAGAGCATCCAG; GTTGCCCATCGTCTTTACTGGCTGCTAAAAAATGCAGAAAATGAAGCTTATTTTAAAAGCTGGTATCAG; AAGCTACTAGCTGCTCTCCAATTCTGTGCAGGTAAAGCCTTGAATGATGAGTTTTCCAAGGAGCAGAAA; CTTATCAAAATTCTGGGAGATATTGGGGAAAGAGTCAAGTCTGCCAGTGACCATCAAAGACAGGAGGTA; CTGAAGAAAGAAATTGGCAGACTAGAAGAGTTCTTTCAAGATGTAAATACTTGTCATCTTCCTCTGAAC; CCTGCCCTATGTATAAAAGGGATTGATCACGATGCATGTTCATATTTTACATCTAATGCTTTGCCATTG; AAGATTACTTTCATCAATGCTAATCCGATGGGCAAAAACATCAGCATTATTTTTAAGGCTGGAGATGAT; CTTCGTCAGGATATGCTTGTTCTGCAGCTTATTCAAGTGATGGACAATATTTGGCTGCAGGAAGGCTTG; GATATGCAAATGATCATTTATAGATGTCTATCCACAGGAAAAGACCAAGGATTGGTGCAGATGGTACCT; GATGCTGTGACCCTAGCAAAGATTCATCGCCATTCTGGACTGATAGGACCATTGAAAGAAAATACAATT; AAAAAGTGGTTCAGTCAGCACAACCACTTAAAGGCAGATTATGAAAAGGCCTTGAGGAACTTTTTCTAC; TCCTGTGCTGGCTGGTGTGTGGTAACATTCATCCTGGGAGTATGTGACCGTCACAATGATAATATCATG; CTGACAAAGTCGGGCCACATGTTTCATATTGACTTTGGAAAATTCTTAGGTCATGCACAAACATTTGGA; GGGATAAAAAGGGACCGAGCTCCTTTCATTTTTACTTCAGAGATGGAATACTTTATTACAGAGGGTGGG; AAAAACCCACAGCATTTTCAAGATTTTGTGGAACTTTGCTGTCGTGCTTATAATATTATCAGAAAGCAC; AGCCAACTGCTCTTGAACCTGCTGGAAATGATGCTGTATGCAGGACTGCCTGAGCTAAGTGGAATTCAA; GACCTGAAATATGTGTATAATAATCTTCGTCCACAAGACACAGACCTGGAAGCAACAAGTCATTTTACC; AAGAAAATAAAGGAAAGTCTGGAGTGTTTCCCTGTTAAATTGAATAACTTGATCCACACACTTGCACAA; ATGTCAGCCATAAGCCCTGCCAAATCTACTTCACAGACTTTTCCTCAGGAATCCTGTTTGCTGAGTACA; ACTAGGTCGATTGAAAGAGCAACAATTTTAGGGTTCAGCAAGAAATCCAGTAATCTGTATCTGATCCAG; GTGACACACAGCAACAACGAAACAAGCCTGACAGAAAAATCATTTGAGCAGTTTTCAAAACTTCACAGC; CAACTTCAGAAGCAGTTTGCATCACTGACTCTCCCAGAGTTTCCTCATTGGTGGCACCTACCTTTTACA; AATTCAGATCACAGAAGATTCAGAGATCTAAATCATTACATGGAACAGATATTAAATGTATCACATGAA; GTTACAAACAGTGATTGTGTACTTAGCTTTTTCCTCTCTGAGGCTGTGCAACAAACAGTTGAAGAATCA; TCACCTGTGTACCTAGGTGAGAAGTTTCCAGACAAGAAGCCTAAGGTGCAGTTAGTCATATCCTACGAG; GATGTGAAGCTGACCATACTAGTGAAACACATGAAAAACATTCATCTCCCAGATGGCTCTGCGCCCAGT; GCACATGTTGAATTTTATCTTTTACCATATCCCAGTGAAGTTCGTAGGAGGAAAACAAAATCTGTTCCA; AAATGTACGGACCCCACTTACAATGAAATTGTAGTATATGATGAAGTCACAGAGCTCCAAGGACATGTC; TTAATGCTTATTGTGAAGAGTAAAACTGTATTTGTGGGAGCAATTAACATCCGACTCTGTAGTGTCCCA; CTCGATAAAGAAAAATGGTATCCATTAGGAAACAGTATAATT; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG240163; Blue=ORF Red=Cloning site Green=Tag(s); ; MAYSWQTDPNPNESHEKQYEHQEFLFVNQPHSSSQVSLGFDQIVDEISGKIPHYESEIDENTFFVPTAP; KWDSTGHSLNEAHQISLNEFTSKSRELSWHQVSKAPAIGFSPSVLPKPQNTNKECSWGSPIGKHHGADD; SRFSILAPSFTSLDKINLEKELENENHNYHIGFESSIPPTNSSFSSDFMPKEENKRSGHVNIVEPSLML; LKGSLQPGMWESTWQKNIESIGCSIQLVEVPQSSNTSLASFCNKVKKIRERYHAADVNFNSGKIWSTTT; AFPYQLFSKTKFNIHIFIDNSTQPLHFMPCANYLVKDLIAEILHFCTNDQLLPKDHILSVCGSEEFLQN; DHCLGSHKMFQKDKSVIQLHLQKSREAPGKLSRKHEEDHSQFYLNQLLEFMHIWKVSRQCLLTLIRKYD; FHLKYLLKTQENVYNIIEEVKKICSVLGCVETKQITDAVNELSLILQRKGENFYQSSETSAKGLIEKVT; TELSTSIYQLINVYCNSFYADFQPVNVPRCTSYLNPGLPSHLSFTVYAAHNIPETWVHSYKAFSFTCWL; TYAGKKLCQVRNYRNIPDKKLFFFLVNWNETINFPLEIKSLPRESMLTVKLFGIACATNNANLLAWTCL; PLFPKEKSILGSMLFSMTLQSEPPVEMITPGVWDVSQPSPVTLQIDFPATGWEYMKPDSEENRSNLEEP; LKECIKHIARLSQKQTPLLLSEEKKRYLWFYRFYCNNENCSLPLVLGSAPGWDERTVSEMHTILRRWTF; SQPLEALGLLTSSFPDQEIRKVAVQQLDNLLNDELLEYLPQLVQAVKFEWNLESPLVQLLLHRSLQSIQ; VAHRLYWLLKNAENEAYFKSWYQKLLAALQFCAGKALNDEFSKEQKLIKILGDIGERVKSASDHQRQEV; LKKEIGRLEEFFQDVNTCHLPLNPALCIKGIDHDACSYFTSNALPLKITFINANPMGKNISIIFKAGDD; LRQDMLVLQLIQVMDNIWLQEGLDMQMIIYRCLSTGKDQGLVQMVPDAVTLAKIHRHSGLIGPLKENTI; KKWFSQHNHLKADYEKALRNFFYSCAGWCVVTFILGVCDRHNDNIMLTKSGHMFHIDFGKFLGHAQTFG; GIKRDRAPFIFTSEMEYFITEGGKNPQHFQDFVELCCRAYNIIRKHSQLLLNLLEMMLYAGLPELSGIQ; DLKYVYNNLRPQDTDLEATSHFTKKIKESLECFPVKLNNLIHTLAQMSAISPAKSTSQTFPQESCLLST; TRSIERATILGFSKKSSNLYLIQVTHSNNETSLTEKSFEQFSKLHSQLQKQFASLTLPEFPHWWHLPFT; NSDHRRFRDLNHYMEQILNVSHEVTNSDCVLSFFLSEAVQQTVEESSPVYLGEKFPDKKPKVQLVISYE; DVKLTILVKHMKNIHLPDGSAPSAHVEFYLLPYPSEVRRRKTKSVPKCTDPTYNEIVVYDEVTELQGHV; LMLIVKSKTVFVGAINIRLCSVPLDKEKWYPLGNSII; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288772
• ORF Size: 4523 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001288772.2
• RefSeq Size: 4971 bp
• RefSeq ORF: 4461 bp
• Locus ID: 5288
• UniProt ID: O75747
• Cytogenetics: 12p12.3
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 171.1 kDa
• Summary: The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. This gene may play a role in several diseases, including type II diabetes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]