USP25 (NM_001283042) Human Tagged ORF Clone

SKU RG239955

USP25 (tGFP-tagged) - Human ubiquitin specific peptidase 25 (USP25), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: USP25
• Synonyms: USP21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239955 representing NM_001283042.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAA; CTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAAC; TTGGAATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGAGGAGACAACTTAC; TACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCAGATACAAATGTGATT; GATCTCACTGGAGATGATAAAGATGATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAGG; GCATTCAGGGAGACTGGAATAACTGATGAGGAACAAGCCATTAGCAGAGTTCTTGAAGCCAGCATAGCA; GAGAATAAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGA; AAAAGACAGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTTAGTGCTGTTATT; CAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTGAATTACAAGCCTCCATCAAATGCTCAA; GATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCTTTTATGCGTGAGCTGAGGTATCTATTTGCA; CTTCTTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTC; AAATCAAATGACTCACAGCAGCAAGATGTGAGTGAGTTTACACACAAATTATTAGATTGGTTAGAAGAT; GCCTTCCAAATGAAAGCTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTGTTC; TATGGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGAAAACACTGAAATGTTTGGTCAG; TACCCACTTCAGGTCAATGGGTTCAAAGATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAAGGAGAA; ATTGAGTCTTTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGAATTACCACCT; GTGTTAACATTTGAATTGTCAAGATTTGAATTTAATCAGGCATTGGGAAGACCAGAAAAAATTCACAAC; AAATTAGAATTTCCCCAAGTTTTATATTTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATT; AAGAGGGAAGAGATCAAGAGACTGAAAGATTACCTCACGGTATTACAACAAAGGCTAGAAAGATATTTA; AGCTATGGTTCCGGTCCCAAACGATTCCCCTTGGTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCA; AGTAAACCTGTTTGCACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCATACCA; TCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCTTCAGAACTGCCAAGCACATCACCT; TCATCAGTTGCTGCCATTTCATCGAGATCAGTAATACACAAACCATTTACTCAGTCCCGGATACCTCCA; GATTTGCCCATGCATCCGGCACCAAGGCACATAACGGAGGAAGAACTTTCTGTGCTGGAAAGTTGTTTA; CATCGCTGGAGGACAGAAATAGAAAATGACACCAGAGATTTGCAGGAAAGCATATCCAGAATCCATCGA; ACAATTGAATTAATGTACTCTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTT; CACGAAGGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCATCGTGAAAGCAGATGGATG; AAGTACAATGATATTGCTGTGACAAAATCATCATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGTTAT; AGAAATGCCAGTGCATACTGTTTAATGTACATAAATGATAAGGCACAGTTCCTAATACAAGAGGAGTTT; AATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACATTACCACCGGATTTGAGAGATTTTGTTGAG; GAAGACAACCAACGATTTGAAAAAGAACTAGAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAG; GAAAAGCTTTTAGCGTCTCAGAAATTGAGAGAGTCAGAGACTTCTGTGACAACAGCACAAGCAGCAGGA; GACCCAGAATATCTAGAGCAGCCATCAAGAAGTGATTTCTCAAAGCACTTGAAAGAAGAAACTATTCAA; ATAATTACCAAGGCATCACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGATCATGATG; ACACCGAACATGCAAGGTATTATCATGGCGATAGGTAAATCCAGGAGTGTATATGACAGGTGTGGCCCT; GAAGCAGGGTTCTTTAAGGCAATTAAGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACC; CCACCAGAAACCGATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCAGGCACCAAAGAAA; ATTATTGAGAAAACATTACTAGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGTCACAAC; ATAATGAAAGTTGCTCAAGCCAAACTGGAAATGATAAAACCTGAAGAAGTAAACTTGGAGGAATATGAG; GAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTATCTCATAATTGGGCTAGAAAATTTT; CAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTCATCTGTGCTTATCAGAATAACAAAGAACTCTTG; TCTAAAGGCTTATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTAAAA; TTAAATGAGCAAGCCGCAGAACTCTTCGAATCTGGAGAGGATCGAGAAGTAAACAATGGTTTGATTATC; ATGAATGAGTTTATTGTCCCATTTTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTA; GCTGTAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAACCACACCTCCAAGAA; AAGCTGACAGATTTTTTGCCAAAACTGCTTGATTGTTCTATGGAGATTAAAAGTTTCCATGAGCCACCG; AAGTTACCTTCATATTCCACGCATGAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGTCGA; ACTCCTGCTGATGGAAGA; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG239955; Blue=ORF Red=Cloning site Green=Tag(s); ; MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTY; YQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIA; ENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQ; DLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLED; AFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGE; IESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRI; KREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIP; SQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCL; HRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWM; KYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVE; EDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQ; IITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDT; PPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYE; EWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLK; LNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQE; KLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001283042
• ORF Size: 3309 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001283042.3
• RefSeq Size: 5245 bp
• RefSeq ORF: 3264 bp
• Locus ID: 29761
• UniProt ID: Q9UHP3
• Cytogenetics: 21q21.1
• Protein Families: Protease
• MW: 126.2 kDa
• Summary: Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]