GRIK5 (NM_001301030) Human Tagged ORF Clone

SKU RG239851

GRIK5 (tGFP-tagged) - Human glutamate receptor, ionotropic, kainate 5 (GRIK5), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GRIK5
• Synonyms: EAA2; GluK5; GRIK2; KA2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239851 representing NM_001301030.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCCGGCTGAGCTGCTGCTGCTGCTGATTGTTGCCTTCGCCAGCCCCAGCTGCCAGGTGCTCTCATCA; CTGCGCATGGCTGCAATCCTGGATGATCAGACAGTGTGTGGCCGCGGTGAGCGTCTGGCCTTGGCCTTG; GCCCGGGAGCAGATCAACGGGATCATCGAGGTCCCAGCCAAGGCCCGAGTGGAAGTAGACATCTTTGAG; CTGCAGCGGGACAGCCAGTACGAGACCACGGACACCATGTGTCAGATCTTACCCAAAGGGGTTGTGTCT; GTCCTTGGGCCCTCCTCTAGCCCAGCATCTGCCTCCACCGTGAGCCATATCTGTGGAGAGAAGGAGATC; CCCCACATCAAGGTGGGTCCCGAGGAGACACCCCGCCTTCAGTACCTTCGCTTCGCGTCTGTCAGCCTG; TACCCCAGTAACGAGGACGTCAGCTTGGCGGTCTCCCGAATCCTCAAGTCCTTCAACTACCCCTCGGCC; AGCCTCATCTGCGCCAAGGCTGAGTGCCTGCTGCGATTGGAGGAACTGGTGCGTGGCTTCCTCATCTCC; AAGGAGACGCTGTCAGTGAGGATGTTGGACGACAGCCGGGACCCCACACCACTGCTCAAGGAGATCCGT; GATGACAAGGTGTCCACCATCATCATCGACGCCAACGCCTCCATCTCCCACCTCATCCTCCGTAAGGCC; TCGGAACTGGGAATGACCTCAGCGTTTTACAAGTACATCCTCACCACCATGGACTTCCCCATCCTGCAT; CTGGACGGTATTGTGGAGGACTCCTCCAACATCCTGGGCTTCTCCATGTTCAACACGTCCCACCCCTTC; TACCCTGAGTTTGTCCGCAGCCTCAACATGTCCTGGAGGGAGAACTGTGAAGCCAGCACCTACCTGGGC; CCTGCGCTGTCAGCCGCCCTGATGTTTGACGCCGTGCACGTGGTGGTGAGCGCTGTCCGAGAGCTGAAC; CGCAGCCAGGAGATCGGTGTGAAGCCTCTGGCCTGTACATCGGCCAACATTTGGCCCCACGGGACCAGC; CTCATGAACTACCTGCGCATGGTAGAGTATGATGGGCTGACCGGGCGGGTCGAGTTCAACAGCAAAGGG; CAGAGAACCAACTACACCCTGCGCATCCTAGAAAAGTCCCGGCAGGGCCACCGTGAGATTGGGGTGTGG; TACTCTAACCGCACCCTGGCCATGAATGCCACCACCCTGGACATCAACCTGTCGCAGACACTGGCCAAC; AAGACCCTGGTGGTCACAACCATCCTGGAGAACCCATACGTCATGCGCCGGCCCAACTTCCAGGCCCTG; TCGGGGAACGAACGCTTCGAGGGCTTCTGCGTGGACATGCTGCGGGAGCTGGCCGAGCTGCTGCGCTTC; CGCTACCGCCTGCGGTTGGTGGAGGATGGGCTGTACGGGGCGCCCGAGCCCAACGGCTCCTGGACGGGC; ATGGTTGGCGAGCTCATCAACCGGAAGGCAGACCTGGCTGTGGCCGCCTTCACCATCACAGCTGAGCGG; GAGAAGGTCATCGACTTTTCCAAGCCCTTTATGACCCTGGGGATCAGCATCCTCTACCGAGTGCACATG; GGCCGCAAGCCTGGCTACTTCTCCTTCCTGGACCCCTTCTCCCCTGCTGTGTGGCTCTTCATGCTTCTT; GCCTACCTGGCTGTCAGCTGCGTCCTGTTTCTGGCTGCCAGGCTGAGCCCCTATGAGTGGTATAACCCA; CACCCATGCCTGCGGGCACGCCCCCACATCCTGGAGAACCAGTACACGCTGGGCAACAGCCTGTGGTTT; CCCGTGGGGGGCTTCATGCAGCAGGGCTCGGAGATCATGCCCCGGGCGCTGTCCACGCGCTGTGTCAGC; GGAGTCTGGTGGGCCTTCACCTTGATCATCATCTCCTCCTACACGGCCAACCTGGCCGCCTTCCTCACC; GTGCAGCGCATGGAGGTGCCTGTGGAGTCGGCCGATGACCTGGCAGATCAGACCAACATCGAGTATGGC; ACCATCCACGCCGGCTCCACCATGACCTTCTTCCAGAATTCACGGTACCAAACGTACCAGCGCATGTGG; AACTACATGCAGTCGAAGCAGCCCAGCGTGTTCGTCAAGAGCACAGAAGAGGGCATTGCCCGCGTCCTC; AACTCCCGCTACGCCTTCCTGCTCGAGTCCACCATGAACGAATACCACCGGCGCCTCAACTGCAACCTC; ACCCAGATCGGGGGACTCCTCGACACCAAGGGCTACGGCATTGGCATGCCGCTGGGCTCCCCGTTCCGG; GATGAGATCACACTGGCCATCCTGCAGCTTCAGGAGAACAACCGGCTGGAGATCCTGAAGCGCAAGTGG; TGGGAGGGGGGCCGGTGCCCCAAGGAGGAGGACCATCGAGCTAAAGGTTTGGGCATGGAGAACATTGGT; GGCATTTTTATCGTGCTCATCTGTGGCCTCATCATTGCTGTCTTCGTGGCGGTCATGGAATTCATATGG; TCCACACGGAGGTCAGCTGAGTCCGAGGAGACCCCAGCTCTGCATCCAGCTGCCTGCCAGTGTTCTGCA; CTTGGCCCAAGGACTCCTCTGAAGGAGCCGTCCATGCTGCTAGTAAAGGTGCCCTCCACCCGTGTCCAG; GTTGCCTTCTCAAGGACATCCCTCCGGCAAGTCTGTCCTTTCCTCCTGCAGCATCAGTTGTCCTCTCTT; TATTGGATTCAGGCCACCAACGTACAAATATGCTGTCATTTCTCCAGTCTTAAACCCTCTCCTGACCTC; ACTTTCCCTCCCAGCCACCGCCCACTTTCTTCCCTTCTCTTTACAGCCTTGGCAGCAGTGGGGGGTCTC; CCTGATGCTTCCAGTTTCTTCTTTCCTCCCATTTCCTCTTGCCCCCCACTCCAGTCTGGCATCGGTCCC; TGCCACTCCACTGAAGCCACTCTTGTCACCAGCAACTTCCATGTC; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG239851; Blue=ORF Red=Cloning site Green=Tag(s); ; MPAELLLLLIVAFASPSCQVLSSLRMAAILDDQTVCGRGERLALALAREQINGIIEVPAKARVEVDIFE; LQRDSQYETTDTMCQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVGPEETPRLQYLRFASVSL; YPSNEDVSLAVSRILKSFNYPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEIR; DDKVSTIIIDANASISHLILRKASELGMTSAFYKYILTTMDFPILHLDGIVEDSSNILGFSMFNTSHPF; YPEFVRSLNMSWRENCEASTYLGPALSAALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTS; LMNYLRMVEYDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLAN; KTLVVTTILENPYVMRRPNFQALSGNERFEGFCVDMLRELAELLRFRYRLRLVEDGLYGAPEPNGSWTG; MVGELINRKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLL; AYLAVSCVLFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQGSEIMPRALSTRCVS; GVWWAFTLIIISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMW; NYMQSKQPSVFVKSTEEGIARVLNSRYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFR; DEITLAILQLQENNRLEILKRKWWEGGRCPKEEDHRAKGLGMENIGGIFIVLICGLIIAVFVAVMEFIW; STRRSAESEETPALHPAACQCSALGPRTPLKEPSMLLVKVPSTRVQVAFSRTSLRQVCPFLLQHQLSSL; YWIQATNVQICCHFSSLKPSPDLTFPPSHRPLSSLLFTALAAVGGLPDASSFFFPPISSCPPLQSGIGP; CHSTEATLVTSNFHV; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301030
• ORF Size: 2986 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001301030.1, NP_001287959.1
• RefSeq Size: 3331 bp
• RefSeq ORF: 2946 bp
• Locus ID: 2901
• UniProt ID: Q16478
• Cytogenetics: 19q13.2
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 110.1 kDa
• Summary: This gene encodes a protein that belongs to the glutamate-gated ionic channel family. Glutamate functions as the major excitatory neurotransmitter in the central nervous system through activation of ligand-gated ion channels and G protein-coupled membrane receptors. The protein encoded by this gene forms functional heteromeric kainate-preferring ionic channels with the subunits encoded by related gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]