MRTFA (NM_001282660) Human Tagged ORF Clone

SKU RG239794

MKL1 (tGFP-tagged) - Human megakaryoblastic leukemia (translocation) 1 (MKL1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MRTFA
• Synonyms: BSAC; MAL; MKL; MKL1; MRTF-A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239794 representing NM_001282660.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCCGCCTTTGAAAAGTCCAGCCGCATTTCATGAGCAGAGAAGGAGCTTGGAGCGGGCCAGGACAGAG; GACTATCTCAAACGGAAGATTCGTTCCCGGCCGGAGAGATCGGAGCTGGTCAGGATGCACATTTTGGAA; GAGACCTCGGCTGAGCCATCCCTCCAGGCCAAGCAGCTGAAGCTGAAGAGAGCCAGACTAGCCGATGAC; CTCAATGAGAAGATTGCACAGAGGCCTGGCCCCATGGAGCTGGTGGAGAAGAACATCCTTCCTGTTGAG; TCCAGCCTGAAGGAAGCCATCATTGTGGGCCAGGTGAACTATCCCAAAGTAGCAGACAGCTCTTCCTTC; GATGAGGACAGCAGCGATGCCTTATCCCCCGAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCTGTGCCG; TCACCCCTGGAGGCCCGAGTCAGCGAACCACTGCTCAGTGCCACCTCTGCATCCCCCACCCAGGTTGTG; TCTCAACTTCCGATGGGCCGGGATTCCAGAGAAATGCTTTTCCTGGCAGAGCAGCCTCCTCTGCCTCCC; CCACCTCTGCTGCCTCCCAGCCTCACCAATGGAACCACTATCCCCACTGCCAAGTCCACCCCCACACTC; ATTAAGCAAAGCCAACCCAAGTCTGCCAGTGAGAAGTCACAGCGCAGCAAGAAGGCCAAGGAGCTGAAG; CCAAAGGTGAAGAAGCTCAAGTACCACCAGTACATCCCCCCGGACCAGAAGCAGGACAGGGGGGCACCC; CCCATGGACTCATCCTACGCCAAGATCCTGCAGCAGCAGCAGCTCTTCCTCCAGCTGCAGATCCTCAAC; CAGCAGCAGCAGCAGCACCACAACTACCAGGCCATCCTGCCTGCCCCGCCAAAGTCAGCAGGCGAGGCC; CTGGGAAGCAGCGGGACCCCCCCAGTACGCAGCCTCTCCACTACCAATAGCAGCTCCAGCTCGGGCGCC; CCTGGGCCCTGTGGGCTGGCACGTCAGAACAGCACCTCACTGACTGGCAAGCCGGGAGCCCTGCCGGCC; AACCTGGACGACATGAAGGTGGCAGAGCTGAAGCAGGAGCTGAAGTTGCGATCACTGCCTGTCTCGGGC; ACCAAAACTGAGCTGATTGAGCGCCTTCGAGCCTATCAAGACCAAATCAGCCCTGTGCCAGGAGCCCCC; AAGGCCCCTGCCGCCACCTCTATCCTGCACAAGGCTGGCGAGGTGGTGGTAGCCTTCCCAGCGGCCCGG; CTGAGCACGGGGCCAGCCCTGGTGGCAGCAGGCCTGGCTCCAGCTGAGGTGGTGGTGGCCACGGTGGCC; AGCAGTGGGGTGGTGAAGTTTGGCAGCACGGGCTCCACGCCCCCCGTGTCTCCCACCCCCTCGGAGCGC; TCACTGCTCAGCACGGGCGATGAAAACTCCACCCCCGGGGACACCTTTGGTGAGATGGTGACATCACCT; CTGACGCAGCTGACCCTGCAGGCCTCGCCACTGCAGATCCTCGTGAAGGAGGAGGGCCCCCGGGCCGGG; TCCTGTTGCCTGAGCCCTGGGGGGCGGGCGGAGCTAGAGGGGCGCGACAAGGACCAGATGCTGCAGGAG; AAAGACAAGCAGATCGAGGCGCTGACGCGCATGCTCCGGCAGAAGCAGCAGCTGGTGGAGCGGCTCAAG; CTGCAGCTGGAGCAGGAGAAGCGAGCCCAGCAGCCCGCCCCCGCCCCCGCCCCCCTCGGCACCCCCGTG; AAGCAGGAGAACAGCTTCTCCAGCTGCCAGCTGAGCCAGCAGCCCCTGGGCCCCGCTCACCCATTCAAC; CCCAGCCTGGCGGCCCCAGCCACCAACCACATAGACCCTTGTGCTGTGGCCCCGGGGCCCCCGTCCGTG; GTGGTGAAGCAGGAAGCCTTGCAGCCTGAGCCCGAGCCGGTCCCCGCCCCCCAGTTGCTTCTGGGGCCT; CAGGGCCCCAGCCTCATCAAGGGGGTTGCACCTCCCACCCTCATCACCGACTCCACAGGGACCCACCTT; GTCCTCACCGTGACCAATAAGAATGCAGACAGCCCTGGCCTGTCCAGTGGGAGCCCCCAGCAGCCCTCG; TCCCAGCCTGGCTCTCCAGCGCCTGCCCCCTCTGCCCAGATGGACCTGGAGCACCCACTGCAGCCCCTC; TTTGGGACCCCCACTTCTCTGCTGAAGAAGGAACCACCTGGCTATGAGGAAGCCATGAGCCAGCAGCCC; AAACAGCAGGAAAATGGTTCCTCAAGCCAGCAGATGGACGACCTGTTTGACATTCTCATTCAGAGCGGA; GAAATTTCAGCAGATTTCAAGGAGCCGCCATCCCTGCCAGGGAAGGAGAAGCCATCCCCGAAGACAGTC; TGTGGGTCCCCCCTGGCAGCACAGCCATCACCTTCTGCTGAGCTCCCCCAGGCTGCCCCACCTCCTCCA; GGCTCACCCTCCCTCCCTGGACGCCTGGAGGACTTCCTGGAGAGCAGCACGGGGCTGCCCCTGCTGACC; AGTGGGCATGACGGGCCAGAGCCCCTTTCCCTCATTGACGACCTCCATAGCCAGATGCTGAGCAGCACT; GCCATCCTGGACCACCCCCCGTCACCCATGGACACCTCGGAATTGCACTTTGTTCCTGAGCCCAGCAGC; ACCATGGGCCTGGACCTGGCTGATGGCCACCTGGACAGCATGGACTGGCTGGAGCTGTCGTCAGGTGGT; CCCGTGCTGAGCCTAGCCCCCCTCAGCACCACAGCCCCCAGCCTCTTCTCCACAGACTTCCTCGATGGC; CATGATTTGCAGCTGCACTGGGATTCCTGCTTG; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG239794; Blue=ORF Red=Cloning site Green=Tag(s); ; MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERSELVRMHILEETSAEPSLQAKQLKLKRARLADD; LNEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVP; SPLEARVSEPLLSATSASPTQVVSQLPMGRDSREMLFLAEQPPLPPPPLLPPSLTNGTTIPTAKSTPTL; IKQSQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDRGAPPMDSSYAKILQQQQLFLQLQILN; QQQQQHHNYQAILPAPPKSAGEALGSSGTPPVRSLSTTNSSSSSGAPGPCGLARQNSTSLTGKPGALPA; NLDDMKVAELKQELKLRSLPVSGTKTELIERLRAYQDQISPVPGAPKAPAATSILHKAGEVVVAFPAAR; LSTGPALVAAGLAPAEVVVATVASSGVVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDTFGEMVTSP; LTQLTLQASPLQILVKEEGPRAGSCCLSPGGRAELEGRDKDQMLQEKDKQIEALTRMLRQKQQLVERLK; LQLEQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAVAPGPPSV; VVKQEALQPEPEPVPAPQLLLGPQGPSLIKGVAPPTLITDSTGTHLVLTVTNKNADSPGLSSGSPQQPS; SQPGSPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQMDDLFDILIQSG; EISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGSPSLPGRLEDFLESSTGLPLLT; SGHDGPEPLSLIDDLHSQMLSSTAILDHPPSPMDTSELHFVPEPSSTMGLDLADGHLDSMDWLELSSGG; PVLSLAPLSTTAPSLFSTDFLDGHDLQLHWDSCL; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282660
• ORF Size: 2834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001282660.2
• RefSeq Size: 4138 bp
• RefSeq ORF: 2796 bp
• Locus ID: 57591
• UniProt ID: Q969V6
• Cytogenetics: 22q13.1-q13.2
• Protein Families: Transcription Factors
• MW: 98.9 kDa
• Summary: The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]