PIF1 (NM_001286496) Human Tagged ORF Clone

SKU RG239183

PIF1 (tGFP-tagged) - Human PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PIF1
• Synonyms: C15orf20; PIF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239183 representing NM_001286496.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCTCTCGGGCATAGAGGCGGCGGCAGGGGAATATGAGGACTCGGAGCTGCGGTGCCGCGTGGCTGTG; GAGGAGCTGAGCCCGGGCGGGCAGCCGCGAAGGCGCCAGGCCCTGCGCACCGCGGAGCTGAGCCTGGGT; CGCAACGAGCGCCGCGAGTTGATGCTGCGGCTGCAAGCGCCAGGGCCCGCGGGGCGGCCGCGCTGCTTC; CCTCTGCGCGCCGCGCGCCTCTTCACGCGTTTCGCCGAGGCCGGGCGCAGCACCCTGCGGCTCCCCGCC; CACGACACCCCCGGGGCCGGCGCAGTGCAGCTGCTGCTCTCGGACTGCCCCCCAGACCGCCTGCGCCGC; TTCCTGCGCACATTGCGCCTCAAGCTGGCTGCGGCCCCGGGTCCCGGGCCGGCCTCCGCCCGAGCGCAG; CTGCTGGGCCCGCGGCCCCGCGACTTCGTCACCATCAGCCCTGTGCAGCCCGAGGAGCGGCGGCTCAGG; GCGGCCACCCGGGTTCCGGACACTACGCTGGTGAAGCGGCCTGTGGAGCCCCAGGCTGGGGCCGAGCCT; AGCACAGAAGCCCCAAGGTGGCCCCTGCCTGTGAAGAGGCTGAGCTTGCCCTCCACCAAGCCACAGCTT; TCTGAGGAACAGGCTGCTGTGCTGAGGGCCGTCCTGAAAGGCCAGAGCATCTTCTTCACTGGGAGTGCA; GGAACAGGGAAGTCATATCTGCTAAAGCGAATCCTGGGCTCACTGCCCCCCACAGGCACTGTGGCCACT; GCCAGCACTGGGGTGGCAGCCTGCCACATCGGGGGCACCACCCTCCATGCCTTTGCAGGCATCGGCTCA; GGCCAGGCTCCTCTAGCCCAGTGTGTGGCCCTGGCCCAAAGGCCAGGCGTGCGGCAGGGCTGGCTGAAC; TGCCAGCGGTTGGTCATTGACGAGATCTCAATGGTGGAGGCAGACCTGTTTGACAAACTGGAGGCCGTG; GCCAGAGCTGTCCGGCAGCAGAACAAGCCATTCGGAGGGATCCAGCTCATCATCTGTGGGGACTTTCTG; CAGCTGCCACCTGTGACCAAGGGCTCCCAGCCCCCACGGTTCTGCTTCCAGTCCAAGAGCTGGAAGAGG; TGTGTGCCAGTGACCCTGGAGCTGACCAAGGTGTGGAGGCAGGCAGACCAGACCTTCATCTCTCTACTG; CAGGCCGTGAGGCTAGGCAGGTGTTCAGATGAGGTGACCCGCCAGCTCCAGGCCACAGCTTCCCACAAG; GTGGGGCGAGATGGGATTGTGGCCACGAGGCTCTGCACCCACCAGGATGATGTGGCCCTCACCAACGAG; AGGCGGCTTCAGGAGCTGCCAGGTAAGGTACACAGATTTGAGGCTATGGACAGCAACCCTGAGCTGGCC; AGTACCCTGGATGCCCAGTGTCCTGTTAGCCAGCTCCTTCAACTAAAGCTGGGGGCCCAGGTGATGCTG; GTGAAAAACTTATCGGTGTCTCGGGGCCTGGTGAATGGTGCCCGAGGGGTGGTAGTTGGGTTCGAGGCA; GAAGGGAGAGGGCTACCCCAGGTGCGGTTCCTGTGTGGAGTCACTGAGGTCATCCACGCTGACCGCTGG; ACGGTGCAGGCCACCGGGGGCCAGCTCCTCAGTCGGCAGCAGCTGCCCCTCCAGCTGGCCTGGGCGATG; TCCATCCACAAGAGCCAAGGCATGACCCTGGATTGTGTGGAGATTTCTCTGGGCCGTGTGTTTGCCAGT; GGCCAGGCCTATGTGGCCCTTTCTCGGGCCCGCAGCCTGCAGGGCCTACGTGTGCTGGACTTTGACCCC; ATGGCGGTTCGCTGTGACCCCCGTGTGCTGCACTTCTATGCCACCCTGCGGCGGGGCAGGAGCCTCAGT; CTGGAGTCCCCAGATGATGATGAGGCAGCCTCAGACCAGGAGAACATGGACCCAATCCTC; AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG239183; Blue=ORF Red=Cloning site Green=Tag(s); ; MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCF; PLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQ; LLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQL; SEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGS; GQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFL; QLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHK; VGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVML; VKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAM; SIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLS; LESPDDDEAASDQENMDPIL; SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL; SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF; PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP; MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286496
• ORF Size: 1953 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001286496.1, NP_001273425.1
• RefSeq Size: 2718 bp
• RefSeq ORF: 1926 bp
• Locus ID: 80119
• UniProt ID: Q9H611
• Cytogenetics: 15q22.31
• MW: 69.8 kDa
• Summary: This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013]