BMAL1 (ARNTL) (NM_001297722) Human Tagged ORF Clone

SKU RG239140

ARNTL (tGFP-tagged) - Human aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 5

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: BMAL1
• Synonyms: bHLHe5; BMAL1; BMAL1c; JAP3; MOP3; PASD3; TIC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239140 representing NM_001297722.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCAGACCAGAGAATGGACATTTCTTCAACCATCAGTGATTTCATGTCCCCGGGCCCCACCGACCTG; CTTTCCAGCTCTCTTGGTACCAGTGGTGTGGATTGCAACCGCAAACGGAAAGGCAGCTCCACTGACTAC; CAAGAAAGCATGGACACAGACAAAGATGACCCTCATGGAAGGTTAGAATATACAGAACACCAAGGAAGG; ATAAAAAATGCAAGGGAAGCTCACAGTCAGATTGAAAAGCGGCGTCGGGATAAAATGAACAGTTTTATA; GATGAATTGGCTTCTTTGGTACCAACATGCAACGCAATGTCCAGGAAATTAGATAAACTTACTGTGCTA; AGGATGGCTGTTCAGCACATGAAAACATTAAGAGGTGCCACCAATCCATACACAGAAGCAAACTACAAA; CCAACTTTTCTATCAGACGATGAATTGAAACACCTCATTCTCAGGGCAGCAGATGGATTTTTGTTTGTC; GTAGGATGTGACCGAGGGAAGATACTCTTTGTCTCAGAGTCTGTCTTCAAGATCCTCAACTACAGCCAG; AATGATCTGATTGGTCAGAGTTTGTTTGACTACCTGCATCCTAAAGATATTGCCAAAGTCAAGGAGCAG; CTCTCCTCCTCTGACACCGCACCCCGGGAGCGGCTCATAGATGCAAAAACTGGACTTCCAGTTAAAACA; GATATAACCCCTGGGCCATCTCGATTATGTTCTGGAGCACGACGTTCTTTCTTCTGTAGGATGAAGTGT; AACAGGCCTTCAGTAAAGGTTGAAGACAAGGACTTCCCCTCTACCTGCTCAAAGAAAAAAGCAGATCGA; AAAAGCTTCTGCACAATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCCACAAAGATGGGGCTGGAT; GAAGACAACGAACCAGACAATGAGGGGTGTAACCTCAGCTGCCTCGTCGCAATTGGACGACTGCATTCT; CATGTAGTTCCACAACCAGTGAACGGGGAAATCAGGGTGAAATCTATGGAATATGTTTCTCGGCACGCG; ATAGATGGAAAGTTTGTTTTTGTAGACCAGAGGGCAACAGCTATTTTGGCATATTTACCACAAGAACTT; CTAGGCACATCGTGTTATGAATATTTTCACCAAGATGACATAGGACATCTTGCAGAATGTCATAGGCAA; GTTTTACAGACGAGAGAAAAAATTACAACTAATTGCTATAAATTTAAAATCAAAGATGGTTCTTTTATC; ACACTACGGAGTCGATGGTTCAGTTTCATGAACCCTTGGACCAAGGAAGTAGAATATATTGTCTCAACT; AACACTGTTGTTTTAGCCAACGTCCTGGAAGGCGGGGACCCAACCTTCCCACAGCTCACAGCATCCCCC; CACAGCATGGACAGCATGCTGCCCTCTGGAGAAGGTGGCCCAAAGAGGACCCACCCCACTGTTCCAGGG; ATTCCAGGGGGAACCCGGGCTGGGGCAGGAAAAATAGGCCGAATGATTGCTGAGGAAATCATGGAAATC; CACAGGATAAGAGGGTCATCGCCTTCTAGCTGTGGCTCCAGCCCATTGAACATCACGAGTACGCCTCCC; CCTGATGCCTCTTCTCCAGGAGGCAAGAAGATTTTAAATGGAGGGACTCCAGACATTCCTTCCAGTGGC; CTACTATCAGGCCAGGCTCAGGAGAACCCAGGTTATCCATATTCTGATAGTTCTTCTATTCTTGGTGAG; AACCCCCACATAGGTATAGACATGATTGACAACGACCAAGGATCAAGTAGTCCCAGTAATGATGAGGCA; GCAATGGCTGTCATCATGAGCCTCTTGGAAGCAGATGCTGGACTGGGTGGCCCTGTTGACTTTAGTGAC; TTGCCATGGCCGCTG; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG239140; Blue=ORF Red=Cloning site Green=Tag(s); ; MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESMDTDKDDPHGRLEYTEHQGR; IKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYK; PTFLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQ; LSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFPSTCSKKKADR; KSFCTIHSTGYLKSWPPTKMGLDEDNEPDNEGCNLSCLVAIGRLHSHVVPQPVNGEIRVKSMEYVSRHA; IDGKFVFVDQRATAILAYLPQELLGTSCYEYFHQDDIGHLAECHRQVLQTREKITTNCYKFKIKDGSFI; TLRSRWFSFMNPWTKEVEYIVSTNTVVLANVLEGGDPTFPQLTASPHSMDSMLPSGEGGPKRTHPTVPG; IPGGTRAGAGKIGRMIAEEIMEIHRIRGSSPSSCGSSPLNITSTPPPDASSPGGKKILNGGTPDIPSSG; LLSGQAQENPGYPYSDSSSILGENPHIGIDMIDNDQGSSSPSNDEAAMAVIMSLLEADAGLGGPVDFSD; LPWPL; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001297722
• ORF Size: 1906 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001297722.1, NP_001284651.1
• RefSeq Size: 2811 bp
• RefSeq ORF: 1881 bp
• Locus ID: 406
• UniProt ID: O00327
• Cytogenetics: 11p15.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 69.2 kDa
• Summary: The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]