Pericentrin 1 (NUP85) (NM_001303276) Human Tagged ORF Clone

SKU RG239078

NUP85 (tGFP-tagged) - Human nucleoporin 85kDa (NUP85), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Pericentrin 1
• Synonyms: FROUNT; NPHS17; Nup75
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239078 representing NM_001303276.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGTGCCAAGTTGCCCCTTTATCTATATCATCCGTAAGGATGTAGATGTTTACTCTCAAATCTTGAGA; AAACTCTTCAATGAATCCCATGGAATCTTTCTGGGCCTCCAGAGAATTGACGAAGAGTTGACTGGAAAA; TCCAGAAAATCTCAATTGGTTCGAGTGAGTAAAAACTACCGATCAGTCATCAGAGCATGTATGGAGGAA; ATGCACCAGGTTGCAATTGCTGCTAAAGATCCAGCCAATGGCCGCCAGTTCAGCAGCCAGGTCTCCATT; TTGTCAGCAATGGAGCTCATCTGGAACCTGTGTGAGATTCTTTTTATTGAAGTGGCCCCAGCTGGCCCT; CTCCTCCTCCATCTCCTTGACTGGGTCCGGCTCCATGTGTGCGAGGTGGACAGTTTGTCGGCAGATGTT; CTGGGCAGTGAGAATCCAAGCAAACATGACAGCTTCTGGAACTTGGTGACCATCTTGGTGCTGCAGGGC; CGGCTGGATGAGGCCCGACAGATGCTCTCCAAGGAAGCCGATGCCAGCCCCGCCTCTGCAGGCATATGC; CGAATCATGGGGGACCTGATGAGGACAATGCCCATTCTTAGTCCCGGGAACACCCAGACACTGACAGAG; CTGGAGCTGAAGTGGCAGCACTGGCACGAGGAATGTGAGCGGTACCTCCAGGACAGCACATTCGCCACC; AGCCCTCACCTGGAGTCTCTCTTGAAGATTATGCTGGGAGACGAAGCTGCCTTGTTAGAGCAGAAGGAA; CTTCTGAGTAATTGGTATCATTTCCTAGTGACTCGGCTCTTGTACTCCAATCCCACAGTAAAACCCATT; GATCTGCACTACTATGCCCAGTCCAGCCTGGACCTGTTTCTGGGAGGTGAGAGCAGCCCAGAACCCCTG; GACAACATCTTGTTGGCAGCCTTTGAGTTTGACATCCATCAAGTAATCAAAGAGTGCAGCATCGCCCTG; AGCAACTGGTGGTTTGTGGCCCACCTGACAGACCTGCTGGACCACTGCAAGCTCCTCCAGTCACACAAC; CTCTATTTCGGTTCCAACATGAGAGAGTTCCTCCTGCTGGAGTACGCCTCGGGACTGTTTGCTCATCCC; AGCCTGTGGCAGCTGGGGGTCGATTACTTTGATTACTGCCCCGAGCTGGGCCGAGTCTCCCTGGAGCTG; CACATTGAGCGGATACCTCTGAACACCGAGCAGAAAGCCCTGAAGGTGCTGCGGATCTGTGAGCAGCGG; CAGATGACTGAACAAGTTCGCAGCATTTGTAAGATCTTAGCCATGAAAGCCGTCCGCAACAATCGCCTG; GGTTCTGCCCTCTCTTGGAGCATCCGTGCTAAGGATGCCGCCTTTGCCACGCTCGTGTCAGACAGGTTC; CTCAGGGATTACTGTGAGCGAGGCTGCTTTTCTGATTTGGATCTCATTGACAACCTGGGGCCAGCCATG; ATGCTCAGTGACCGACTGACATTCCTGGGAAAGTATCGCGAGTTCCACCGTATGTACGGGGAGAAGCGT; TTTGCCGACGCAGCTTCTCTCCTTCTGTCCTTGATGACGTCTCGGATTGCCCCTCGGTCTTTCTGGATG; ACTCTGCTGACAGACGCCTTGCCCCTTTTGGAACAGAAACAGGTGATTTTCTCAGCAGAACAGACTTAT; GAGTTGATGCGGTGTCTGGAGGACTTGACGTCAAGAAGACCTGTGCATGGAGAATCTGATACCGAGCAG; CTCCAGGATGATGACATAGAGACCACCAAGGTGGAAATGCTGAGACTTTCTCTGGCACGAAATCTTGCT; CGGGCAATTATAAGAGAAGGCTCACTGGAAGGTTCC; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG239078; Blue=ORF Red=Cloning site Green=Tag(s); ; MVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFLGLQRIDEELTGKSRKSQLVRVSKNYRSVIRACMEE; MHQVAIAAKDPANGRQFSSQVSILSAMELIWNLCEILFIEVAPAGPLLLHLLDWVRLHVCEVDSLSADV; LGSENPSKHDSFWNLVTILVLQGRLDEARQMLSKEADASPASAGICRIMGDLMRTMPILSPGNTQTLTE; LELKWQHWHEECERYLQDSTFATSPHLESLLKIMLGDEAALLEQKELLSNWYHFLVTRLLYSNPTVKPI; DLHYYAQSSLDLFLGGESSPEPLDNILLAAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCKLLQSHN; LYFGSNMREFLLLEYASGLFAHPSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKALKVLRICEQR; QMTEQVRSICKILAMKAVRNNRLGSALSWSIRAKDAAFATLVSDRFLRDYCERGCFSDLDLIDNLGPAM; MLSDRLTFLGKYREFHRMYGEKRFADAASLLLSLMTSRIAPRSFWMTLLTDALPLLEQKQVIFSAEQTY; ELMRCLEDLTSRRPVHGESDTEQLQDDDIETTKVEMLRLSLARNLARAIIREGSLEGS; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303276
• ORF Size: 1857 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001303276.1, NP_001290205.1
• RefSeq Size: 2285 bp
• RefSeq ORF: 1833 bp
• Locus ID: 79902
• UniProt ID: Q9BW27
• Cytogenetics: 17q25.1
• MW: 70.2 kDa
• Summary: This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]