Deformed Epidermal Autoregulatory Factor 1 (DEAF1) (NM_001293634) Human Tagged ORF Clone

SKU RG238531

DEAF1 (tGFP-tagged) - Human DEAF1 transcription factor (DEAF1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Deformed Epidermal Autoregulatory Factor 1
• Synonyms: MRD24; NEDHELS; NUDR; SPN; VSVS; ZMYND5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238531 representing NM_001293634.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGAGGACTCGGACTCGGCGGCAAAGCAGCTGGGCCTGGCTGAGGCGGCGGCGGTGGCGGCCGCGGCC; GCTGTGGCGGCGGCGGCCGCGGCCGCGGCAGGAGGCGAGGCGGAGGAGCCGGTGCTGAGCAGGGACGAG; GACTCGGAGGAGGACGCAGACTCGGAGGCGGAGCGGGAGACGCCGCGGGTCACGGCAGTGGCGGTGATG; GCGGCGGAGCCCGGGCACATGGACATGGGCGCCGAGGCCCTGCCCGGCCCCGACGAGGCCGCCGCTGCC; GCAGCCTTCGCAGAGGTGACCACAGTGACAGTGGCCAACGTGGGGGCTGCTGCAGACAATGTCTTCACC; ACGTCTGTGGCGAACGCGGCATCCATCTCAGGACATGTTCTGTCTGGTAGGACGGCCCTTCAGATCGGG; GACAGCCTGAACACCGAAAAAGCGACACTGATTGTCGTCCACACAGATGGGAGCATCGTGGAGACCACC; GGGCTGAAAGGCCCGGCAGCTCCCCTCACCCCAGGTCCTCAGTCTCCTCCAACCCCTCTGGCTCCCGGC; CAAGAAAAAGGTGGAACTAAATACAACTGGGACCCTTCTGTGTACGACAGTGAGCTGCCCGTACGGTGC; CGGAACATCAGCGGCACTCTGTACAAGAACAGGCTCGGCTCAGGATGGGATCTTAAACCCTCACGCTGC; CTCTTGCACCTGTGCTGCCTGCTGCGACGACATGACCTTATCACCGTGACCCCCTCGGGACAGATCACG; ACCTCGGGGGCACTGACCTTTGACCGAGCGTCCACGGTAGAGGCCACTGCTGTCATATCAGAGAGTCCG; GCCCAGGGCGACGTCTTCGCAGGGGCCACAGTCCAAGAGGCCAGCGTGCAGCCCCCATGCAGGGCCAGC; CACCCTGAGCCTCACTACCCCGGCTATCAGGACAGCTGCCAGATCGCACCGTTTCCAGAAGCTGCGTTG; CCAACGTCACATCCCAAAATAGTGTTGACATCCCTGCCTGCGCTGGCGGTCCCACCCCCGACTCCCACC; AAAGCGGCACCTCCCGCGTTGGTCAATGGGCTGGAGCTGTCAGAGCCGCGGAGCTGGCTGTACCTAGAA; GAGATGGTCAACTCCTTGCTCAACACAGCGCAGCAGCTGAAGACGCTGTTTGAGCAAGCCAAGCATGCC; AGCACCTACCGAGAAGCTGCCACAAACCAGGCCAAGATCCACGCTGACGCAGAGCGGAAGGAGGTGATC; CACCCGCCTCGGCTGCCCAAAGTCCTGGGATTACAGCAGTCCTGCGTTAACTGCGGCCGGGAGGCTATG; AGCGAGTGCACCGGCTGCCACAAGGTCAACTACTGCTCCACCTTCTGCCAACGCAAGGACTGGAAGGAT; CACCAGCACATATGCGGCCAGTCAGCAGCTGTCACCGTCCAGGCAGACGAAGTCCACGTGGCTGAAAGC; GTGATGGAGAAGGTGACCGTG; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG238531; Blue=ORF Red=Cloning site Green=Tag(s); ; MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAGGEAEEPVLSRDEDSEEDADSEAERETPRVTAVAVM; AAEPGHMDMGAEALPGPDEAAAAAAFAEVTTVTVANVGAAADNVFTTSVANAASISGHVLSGRTALQIG; DSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRC; RNISGTLYKNRLGSGWDLKPSRCLLHLCCLLRRHDLITVTPSGQITTSGALTFDRASTVEATAVISESP; AQGDVFAGATVQEASVQPPCRASHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPPTPT; KAAPPALVNGLELSEPRSWLYLEEMVNSLLNTAQQLKTLFEQAKHASTYREAATNQAKIHADAERKEVI; HPPRLPKVLGLQQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHICGQSAAVTVQADEVHVAES; VMEKVTV; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293634
• ORF Size: 1492 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001293634.1, NP_001280563.1
• RefSeq Size: 2523 bp
• RefSeq ORF: 1473 bp
• Locus ID: 10522
• UniProt ID: O75398
• Cytogenetics: 11p15.5
• Protein Families: Secreted Protein, Transcription Factors, Transmembrane
• MW: 51.7 kDa
• Summary: This gene encodes a zinc finger domain-containing protein that functions as a regulator of transcription. The encoded proteins binds to its own promoter as well as to that of several target genes. Activity of this protein is important in the regulation of embryonic development. Mutations in this gene have been found in individuals with autosomal dominant cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]