GAS8 (NM_001286209) Human Tagged ORF Clone

SKU RG238311

GAS8 (tGFP-tagged) - Human growth arrest-specific 8 (GAS8), transcript variant 5

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GAS8
• Synonyms: CILD33; DRC4; GAS11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238311 representing NM_001286209.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAGCAAGGAGCAGGTGGAGGAGCATGTCAGCCGCATCCGGGAGGAGCTGGACCGCGAGCGGGAGGAA; CGAAACTACTTCCAGCTGGAGCGGGACAAGATCCACACCTTCTGGGAGATCACACGGAGGCAGCTGGAG; GAGAAGAAGGCTGAGCTGCGGAACAAAGACCGGGAGATGGAAGAAGCCGAGGAGAGGCACCAGGTGGAG; ATCAAGGTGTACAAGCAGAAAGTGAAGCACCTGCTATATGAGCACCAGAACAACCTGACAGAGATGAAG; GCTGAGGGCACTGTAGTCATGAAGCTGGCACAGAAAGAGCACCGCATACAGGAGAGTGTGCTGCGCAAG; GACATGCGGGCACTGAAGGTGGAGCTCAAGGAGCAGGAGCTGGCCAGTGAGGTGGTGGTGAAGAACCTG; CGGCTGAAACACACCGAGGAGATCACCAGGATGCGGAATGATTTTGAGAGGCAAGTTCGAGAAATTGAG; GCCAAGTATGATAAGAAGATGAAGATGCTGAGGGACGAACTCGACTTGCGGAGAAAGACTGAGCTCCAC; GAAGTGGAGGAGAGGAAGAATGGCCAGATCCACACGCTGATGCAGCGCCACGAGGAGGCCTTCACCGAC; ATTAAGAACTACTACAACGACATCACCCTCAACAACCTGGCCCTCATCAACTCCCTCAAGGAGCAGATG; GAGGACATGCGGAAGAAGGAGGACCACCTGGAGAGGGAGATGGCAGAGGTGTCTGGGCAGAACAAGCGC; CTGGCAGACCCTCTCCAGAAGGCTCGGGAGGAGATGAGCGAGATGCAGAAACAGCTCGCAAACTACGAG; AGGGACAAGCAGATCCTGCTTTGCACAAAAGCCCGTTTGAAAGTCAGGGAGAAAGAGCTGAAAGACCTG; CAGTGGGAGCATGAAGTGTTAGAGCAGCGATTCACCAAGGTGCAGCAGGAGCGGGACGAGCTCTATCGG; AAGTTCACCGCAGCCATCCAGGAGGTGCAGCAGAAGACAGGGTTCAAGAACCTCGTGCTAGAACGCAAG; CTGCAGGCTCTGAGCGCCGCTGTGGAGAAGAAGGAGGTGCAGTTCAACGAGGTCCTGGCTGCCTCTAAC; CTGGACCCTGCAGCCCTGACGCTGGTGTCCCGCAAGCTGGAGGATGTTCTTGAGTCGAAGAACAGCACC; ATCAAGGACCTGCAGTATGAGCTGGCCCAGGTCTGTAAGGCCCATAACGACCTGCTGCGCACGTATGAG; GCAAAGCTGCTGGCCTTCGGGATCCCTCTGGACAACGTGGGCTTCAAGCCCTTGGAAACAGCTGTGATC; GGACAGACACTGGGCCAGGGCCCCGCGGGACTGGTGGGCACCCCGACG; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG238311; Blue=ORF Red=Cloning site Green=Tag(s); ; MSKEQVEEHVSRIREELDREREERNYFQLERDKIHTFWEITRRQLEEKKAELRNKDREMEEAEERHQVE; IKVYKQKVKHLLYEHQNNLTEMKAEGTVVMKLAQKEHRIQESVLRKDMRALKVELKEQELASEVVVKNL; RLKHTEEITRMRNDFERQVREIEAKYDKKMKMLRDELDLRRKTELHEVEERKNGQIHTLMQRHEEAFTD; IKNYYNDITLNNLALINSLKEQMEDMRKKEDHLEREMAEVSGQNKRLADPLQKAREEMSEMQKQLANYE; RDKQILLCTKARLKVREKELKDLQWEHEVLEQRFTKVQQERDELYRKFTAAIQEVQQKTGFKNLVLERK; LQALSAAVEKKEVQFNEVLAASNLDPAALTLVSRKLEDVLESKNSTIKDLQYELAQVCKAHNDLLRTYE; AKLLAFGIPLDNVGFKPLETAVIGQTLGQGPAGLVGTPT; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286209
• ORF Size: 1379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001286209.1, NP_001273138.1
• RefSeq Size: 3200 bp
• RefSeq ORF: 1362 bp
• Locus ID: 2622
• UniProt ID: O95995
• Cytogenetics: 16q24.3
• MW: 54.3 kDa
• Summary: This gene includes 11 exons spanning 25 kb and maps to a region of chromosome 16 that is sometimes deleted in breast and prostrate cancer. The second intron contains an apparently intronless gene, C16orf3, that is transcribed in the opposite orientation. This gene is a putative tumor suppressor gene. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]