SHF (NM_001301170) Human Tagged ORF Clone

SKU RG237229

SHF (tGFP-tagged) - Human Src homology 2 domain containing F (SHF), transcript variant 4

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SHF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237229 representing NM_001301170.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGTTACTGAGCGGAGCTCCTCCGGCTGGCTCCCGCCCGGGGCCGCGAACGCAGGGGAGCGCTGGGGGC; GGCCCGGGGGGGTCCCGCCGGGGCGCCGGGGGTGCGGGAGCCGGCCCAGGAGGGGGCGGCAGCGGCGGA; GTAGCCAAGTGGCTCCGGGAGCACCTGGGCTTCCGCGGGGGGGGCGGCGGCGGAGGGGGCAGCAAGCCG; GCGCCCCCCGAGCCCGACTACCGCCCCCCTGCGCCCTCTCCGGCCGCGCCCCCCGCGCCGCCCCCGGAC; ATCCTGGCCGCCTACAGGCTGCAGCGGGAGCGCGACTTCGAAGACCCCTACTCCGGGGGGTCGTCCGGC; TCCGCCGCCCTCGCCACCCCTGTCGCCCCCGGACCCACGCCGCCCCCGCGCCACGGCTCTCCCCCACAC; CGCCTTATTCGGGTCGAGACCCCGGGGCCCCCGGCGCCGCCTGCTGATGAGCGGATCTCCGGACCCCCC; GCCAGCAGCGATAGGCTAGCTATCCTAGAAGACTATGCGGACCCGTTTGATGTTCAGGAGACTGGCGAA; GGCTCAGCAGGAGCTTCAGGAGCCCCAGAGAAGGTCCCTGAAAATGATGGCTACATGGAGCCCTATGAG; GCTCAAAAGATGATGGCCGAGATCCGGGGCTCCAAGGAGACAGCAACTCAGCCCTTGCCTCTGTATGAC; ACACCCTATGAGCCAGAGGAGGATGGGGCCACCCCGGAAGGTGAGGGGGCCCCCTGGCCCCGGGAGTCC; CGCCTGCCAGAGGATGATGAGAGGCCCCCTGAGGAGTATGACCAGCCCTGGGAGTGGAAGAAGGAGCGG; ATTTCCAAAGCCTTTGCAGGAGCAGCCAGGGATTCATGCACA; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG237229; Blue=ORF Red=Cloning site Green=Tag(s); ; MLLSGAPPAGSRPGPRTQGSAGGGPGGSRRGAGGAGAGPGGGGSGGVAKWLREHLGFRGGGGGGGGSKP; APPEPDYRPPAPSPAAPPAPPPDILAAYRLQRERDFEDPYSGGSSGSAALATPVAPGPTPPPRHGSPPH; RLIRVETPGPPAPPADERISGPPASSDRLAILEDYADPFDVQETGEGSAGASGAPEKVPENDGYMEPYE; AQKMMAEIRGSKETATQPLPLYDTPYEPEEDGATPEGEGAPWPRESRLPEDDERPPEEYDQPWEWKKER; ISKAFAGAARDSCT; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301170
• ORF Size: 883 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001301170.1, NP_001288099.1
• RefSeq Size: 1800 bp
• RefSeq ORF: 873 bp
• Locus ID: 90525
• Cytogenetics: 15q21.1
• MW: 30.3 kDa