PDZD3 (NM_001168468) Human Tagged ORF Clone

SKU RG230192

PDZD3 (tGFP-tagged) - Human PDZ domain containing 3 (PDZD3), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PDZD3
• Synonyms: IKEPP; NHERF4; PDZK2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230192 representing NM_001168468; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGAAAGCCGCAGATCTGCAGGACACAGCCTCGTTAACTCTGAAGTTTAAGTTTAACCCAAAGCTGG; GCATTGATAATCCTGTCCTCTCCCTGGCCGAAGACCACGACCCCTATGATCCCTGGAGCCTGGAGCGGCC; TCGCTTCTGTTTACTGAGCAAAGAGGAGGGCAAGAGTTTTGGCTTCCACCTGCAGCAGGAGCTGGGCAGG; GCTGGGCATGTGGTGTGCAGGGTGGACCCAGGCACCTCTGCCCAGCGCCAGGGTCTTCAGGAAGGAGACA; GGATCCTGGCGGTGAACAATGATGTTGTGGAACACGAAGACTATGCGGTGGTGGTACGCCGCATCCGGGC; CAGCAGCCCTCGGGTGTTGCTGACAGTATTGGCACGGCATGCACATGACGTGGCCCGAGCTCAGCTGGGA; GAAGATGCCCACCTCTGTCCCACCCTAGGCCCAGGGGTCCGGCCCCGGCTGTGCCACATAGTGAAAGATG; AGGGTGGTTTTGGCTTCAGTGTCACCCATGGCAATCAGGGTCCTTTCTGGTTGGTGCTAAGTACTGGAGG; AGCAGCTGAGCGGGCAGGGGTGCCCCCCGGGGCCCGGCTGCTGGAAGTGAATGGGGTCAGTGTGGAGAAG; TTCACTCACAACCAACTCACCAGGAAGCTTTGGCAGAGTGGACAGCAGGTGACCTTGCTGGTGGCAGGGC; CAGAGGTGGAAGAACAGTGTCGCCAGCTGGGATTGCCCCTGGCTGCACCCCTGGCAGAGGGCTGGGCACT; GCCCACCAAGCCCCGCTGCCTGCACCTGGAGAAAGGGCCCCAGGGTTTTGGGTTCCTGCTCCGGGAGGAA; AAGGGCCTTGACGGTCGCCCTGGACAGTTCCTGTGGGAGGTGGACCCGGGACTGCCAGCCAAGAAGGCTG; GGATGCAGGCTGGGGACCGGCTGGTGGCTGTGGCTGGGGAGAGCGTGGAGGGGCTGGGCCATGAGGAGAC; AGTGTCCAGGATCCAGGGGCAGGGCTCCTGTGTCTCCCTCACTGTCGTCGACCCTGAGGCGGACCGCTTC; TTCAGCATGGTTCGCCTGTCCCCACTCCTCTTCTTGGAGAACACAGAGGCTCCCGCCTCGCCCCGGGGCA; GCAGCTCAGCCTCACTGGTTGAGACAGAGGACCCTTCACTTGAAGACACAAGCGTGCCTTCTGTCCCTCT; TGGCTCCCGACAGTGCTTCCTGTACCCTGGGCCTGGTGGCAGCTATGGCTTCCGACTCAGTTGTGTGGCC; AGTGGGCCTCGTCTCTTCATCTCCCAGGTGACTCCAGGAGGCTCAGCTGCCCGGGCTGGGCTGCAAGTGG; GAGACGTGATTCTGGAAGTGAACGGGTATCCTGTTGGGGGACAGAATGACCTGGAGAGGCTTCAGCAGCT; GCCTGAGGCTGAGCCACCCCTCTGCCTGAAGCTGGCAGCCAGGTCTCTGCGGGGCTTGGAAGCCTGGATT; CCCCCTGGGGCTGCAGAGGACTGGGCTCTGGCCTCGGATCTACTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230192 representing NM_001168468; Red=Cloning site Green=Tags(s); ; MEKAADLQDTASLTLKFKFNPKLGIDNPVLSLAEDHDPYDPWSLERPRFCLLSKEEGKSFGFHLQQELGR; AGHVVCRVDPGTSAQRQGLQEGDRILAVNNDVVEHEDYAVVVRRIRASSPRVLLTVLARHAHDVARAQLG; EDAHLCPTLGPGVRPRLCHIVKDEGGFGFSVTHGNQGPFWLVLSTGGAAERAGVPPGARLLEVNGVSVEK; FTHNQLTRKLWQSGQQVTLLVAGPEVEEQCRQLGLPLAAPLAEGWALPTKPRCLHLEKGPQGFGFLLREE; KGLDGRPGQFLWEVDPGLPAKKAGMQAGDRLVAVAGESVEGLGHEETVSRIQGQGSCVSLTVVDPEADRF; FSMVRLSPLLFLENTEAPASPRGSSSASLVETEDPSLEDTSVPSVPLGSRQCFLYPGPGGSYGFRLSCVA; SGPRLFISQVTPGGSAARAGLQVGDVILEVNGYPVGGQNDLERLQQLPEAEPPLCLKLAARSLRGLEAWI; PPGAAEDWALASDLL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168468
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001168468.2
• RefSeq Size: 2287 bp
• RefSeq ORF: 1518 bp
• Locus ID: 79849
• UniProt ID: Q86UT5
• Cytogenetics: 11q23.3
• Protein Families: Druggable Genome
• Summary: Guanylyl cyclase C (GCC, or GUCY2C; MIM 601330) produces cGMP following the binding of either endogenous ligands or heat-stable enterotoxins secreted by E. coli and other enteric bacteria. Activation of GCC initiates a signaling cascade that leads to phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR; MIM 602421), followed by a net efflux of ions and water into the intestinal lumen. IKEPP is a regulatory protein that associates with GCC and regulates the amount of cGMP produced following receptor stimulation (Scott et al., 2002 [PubMed 11950846]).[supplied by OMIM, Mar 2008]