THEMIS (NM_001164685) Human Tagged ORF Clone

SKU
RG228531
THEMIS (tGFP-tagged) - Human thymocyte selection associated (THEMIS), transcript variant 1
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$1,134.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol THEMIS
Synonyms C6orf190; C6orf207; GASP; SPOT; TSEPA
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG228531 representing NM_001164685
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATTATCACTGGAAGAATTCGTCCACTCCCTTGACCTCAGGACCCTACCCAGGGTTCTAGAAATCC
AGGCAGGCATCTATCTTGAAGGCTCTATTTATGAAATGTTTGGAAATGAATGCTGTTTTTCAACAGGAGA
AGTGATTAAAATTACTGGTCTCAAAGTTAAGAAGATCATAGCTGAAATTTGTGAGCAGATTGAAGGTTGT
GAGTCTCTACAGCCATTTGAACTGCCTATGAATTTTCCAGGTCTTTTTAAGATTGTGGCTGATAAAACTC
CATACCTTACTATGGAAGAAATCACAAGGACCATTCATATTGGACCAAGTAGACTAGGGCATCCTTGCTT
CTATCATCAGAAGGATATAAAACTAGAGAACCTCATCATAAAGCAGGGTGAGCAAATCATGCTCAACTCA
GTTGAAGAGATTGATGGAGAAATAATGGTGAGCTGTGCAGTAGCAAGGAATCATCAAACTCACTCATTTA
ATTTGCCTTTGTCACAAGAAGGAGAATTCTACGAGTGTGAAGATGAACGTATTTACACTCTAAAGGAGAT
TGTTGAATGGAAGATTCCTAAGAACAGAACAAGAACTGTAAACCTTACAGATTTTTCAAATAAGTGGGAC
TCAACGAATCCATTTCCTAAAGACTTTTATGGTACCCTGATTCTCAAGCCTGTTTATGAAATTCAAGGTG
TGATGAAATTTCGAAAAGATATAATCCGCATCCTCCCCAGTCTAGATGTCGAAGTCAAAGACATCACTGA
TTCTTACGATGCTAACTGGTTTCTTCAGCTGTTATCAACAGAAGATCTTTTTGAAATGACTAGTAAAGAG
TTCCCCATAGTGACTGAAGTCATAGAAGCACCTGAAGGAAACCACCTGCCCCAAAGCATTTTACAGCCTG
GGAAAACCATTGTGATCCACAAAAAGTACCAGGCATCAAGAATCTTAGCTTCAGAAATTAGAAGCAATTT
TCCTAAAAGACACTTCTTGATCCCCACTAGCTATAAAGGCAAGTTCAAGCGGCGACCGAGGGAGTTCCCA
ACGGCCTATGACCTAGAGATCGCTAAGAGTGAAAAGGAGCCTCTTCACGTGGTGGCCACCAAAGCGTTTC
ATTCCCCTCATGACAAGCTGTCATCCGTATCTGTTGGGGACCAGTTTCTGGTGCATCAGTCAGAGACGAC
TGAAGTCCTCTGTGAGGGAATAAAAAAAGTGGTGAATGTTCTGGCCTGTGAAAAAATCCTCAAAAAGTCC
TATGAGGCTGCGCTGCTCCCTTTGTACATGGAAGGAGGTTTTGTAGAGGTGATTCATGATAAGAAACAGT
ACCCGATTTCTGAGCTCTGTAAACAGTTCCGTTTGCCCTTCAATGTGAAGGTGTCTGTCAGGGATCTTTC
CATTGAAGAGGACGTGTTGGCTGCCACACCAGGACTGCAGTTGGAGGAGGACATTACAGACTCTTACCTA
CTCATAAGTGACTTTGCCAACCCCACGGAGTGCTGGGAAATTCCTGTGGGCCGCTTGAATATGACTGTTC
AGTTAGTTAGTAATTTCTCTAGGGATGCAGAACCATTTCTAGTCAGGACTCTGGTAGAAGAGATCACTGA
AGAGCAATATTACATGATGCGGAGATATGAAAGCTCAGCCTCACATCCCCCACCTCGCCCTCCGAAACAC
CCCTCAGTAGAGGAAACAAAGTTAACCCTGCTAACCTTAGCAGAAGAAAGGACGGTAGACCTGCCCAAGT
CTCCCAAGGCTGGAGTGCAATGGCGTGATCTTGGCTCGCTGCAACCTCTGCCTCCCGGGTTCAAGCAATT
CTCTGCCTCAGCCTCCCACGTAGCTGGGATTACAGGCACCCCCCACCACGTCCAGCGTCATCACGTAGAC
ATAACCAAGAAACTTCACCCAAATCAAGCTGGCCTGGATTCAAAAGTACTGATTGGTAGTCAGAATGATT
TGGTGGATGAAGAGAAAGAAAGGAGCAACCGTGGGGCCACAGCAATAGCAGAAACATTCAAAAATGAAAA
ACATCAAAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG228531 representing NM_001164685
Red=Cloning site Green=Tags(s)

MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGC
ESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNS
VEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWD
STNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKE
FPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFP
TAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKS
YEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYL
LISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKH
PSVEETKLTLLTLAEERTVDLPKSPKAGVQWRDLGSLQPLPPGFKQFSASASHVAGITGTPHHVQRHHVD
ITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001164685
ORF Size 2040 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164685.1, NP_001158157.1
RefSeq Size 3983 bp
RefSeq ORF 2043 bp
Locus ID 387357
UniProt ID Q8N1K5
Cytogenetics 6q22.33
Summary This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.