TMEM64 (NM_001146273) Human Tagged ORF Clone

SKU RG228256

TMEM64 (tGFP-tagged) - Human transmembrane protein 64 (TMEM64), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TMEM64
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228256 representing NM_001146273; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCGGAGCCCGGGCGGGATCCTGCTCCAGGCGCTGCCCCGGCTGCTGCAGCACGCCGCCCTCCCGGGCC; TCGCCGAGCTGCCGGCCCGCTGGGCCCTGCCGCGGGGTGCGGGCGGGGACGGCCCGGCGGACCGCCTTCC; CCGCGGGGGCGGGGCGAGCGCGGCGGCGGCAGCAGCGGCGGCCTCGGGCGCCCTGCTCGGCGCCTATCTG; GAGCGCCACGGTCCGCCCGAGGCTTCGGAGCTGCCGGAGCCGGGCGGGGCCTTGGCGGGCGGCCCCGGGA; GTGGCGGCGGCGGCGTGGTGGTCGGCGTGGCTGAGGTGAGAAACTGGCGCTGCTGCTGCCTCGGCAGCAC; CTGTTGGTGCCGGAGCCTCGTGCTGGTCTGCGTGTTGGCCGCCCTGTGCTTCGCTTCCCTGGCCCTGGTC; CGCCGCTACCTTCACCACCTCCTGCTGTGGGTGGAGAGCCTTGACTCGCTGCTGGGGGTCCTGCTCTTCG; TCGTGGGCTTCATCGTGGTCTCTTTCCCCTGCGGCTGGGGCTACATCGTGCTCAACGTGGCCGCTGGCTA; CCTGTACGGCTTCGTGCTGGGCATGGGTCTGATGATGGTGGGCGTCCTCATCGGCACCTTCATCGCCCAT; GTGGTCTGCAAGCGGCTCCTCACCGCCTGGGTGGCCGCCAGGATCCAGAGCAGCGAGAAGCTGAGCGCGG; TTATTCGCGTAGTGGAGGGAGGAAGCGGCCTGAAAGTGGTGGCGCTGGCCAGACTGACACCCATACCTTT; TGGGCTTCAGAATGCAGTGTTTTCGATTATTATAAGTATAGGCCTCATGTTTTATGTAGTTCATCGAGCT; CAAGTGGAATTGAATGCAGCTATTGTAGCTTGTGAAATGGAACTGAAATCTTCTCTGGTTAAAGGCAATC; AACCAAATACCAGTGGCTCTTCATTCTACAACAAGAGGACCCTAACATTTTCTGGAGGTGGAATCAATGT; TGTA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228256 representing NM_001146273; Red=Cloning site Green=Tags(s); ; MRSPGGILLQALPRLLQHAALPGLAELPARWALPRGAGGDGPADRLPRGGGASAAAAAAAASGALLGAYL; ERHGPPEASELPEPGGALAGGPGSGGGGVVVGVAEVRNWRCCCLGSTCWCRSLVLVCVLAALCFASLALV; RRYLHHLLLWVESLDSLLGVLLFVVGFIVVSFPCGWGYIVLNVAAGYLYGFVLGMGLMMVGVLIGTFIAH; VVCKRLLTAWVAARIQSSEKLSAVIRVVEGGSGLKVVALARLTPIPFGLQNAVFSIIISIGLMFYVVHRA; QVELNAAIVACEMELKSSLVKGNQPNTSGSSFYNKRTLTFSGGGINVV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146273
• ORF Size: 984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001146273.1, NP_001139745.1
• RefSeq Size: 4663 bp
• RefSeq ORF: 987 bp
• Locus ID: 169200
• UniProt ID: Q6YI46
• Cytogenetics: 8q21.3
• Protein Families: Transmembrane
• Summary: Positively regulates TNFSF11-induced osteoclast differentiation. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with SERCA2 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation. Negatively regulates osteoblast differentiation and positively regulates adipocyte differentiation via modulation of the canonical Wnt signaling pathway. Mediates the switch in lineage commitment to osteogenesis rather than to adipogenesis in mesenchymal stem cells by negatively regulating the expression, activity and nuclear localization of CTNNB1.[UniProtKB/Swiss-Prot Function]