GLYCTK (NM_001144951) Human Tagged ORF Clone
SKU
RG227749
GLYCTK (tGFP-tagged) - Human glycerate kinase (GLYCTK), transcript variant 2
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TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Type | Human Tagged ORF Clone |
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Target Symbol | GLYCTK |
Synonyms | HBeAgBP4A; HBEBP2; HBEBP4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RG227749 representing NM_001144951
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGCCCTGCAGGTCCTGCCCCGCTTGGCCCGAGCCCCCTTGCATCCACTCCTCTGGCGGGGCT CAGTGGCCCGTCTGGCCAGCAGCATGGCCTTGGCAGAGCAGGCCAGGCAGCTGTTTGAGAGTGCTGTAGG TGCAGTGCTGCCGGGCCCCATGCTGCACCGGGCACTATCCTTGGACCCTGGTGGCAGACAGCTGAAGGTG CGGGACCGGAACTTTCAGCTGAGGCAAAACCTCTACCTGGTGGGCTTTGGCAAGGCTGTGCTGGGTATGG CAGCTGCAGCTGAGGAACTACTGGGCCAGCATCTTGTGCAGGGCGTGATCAGCGTTCCCAAGGGGATCCG TGCTGCCATGGAGCGTGCCGGCAAGCAGGAGATGCTGCTGAAGCCACATAGCCGTGTCCAGGTATTCGAG GGTGCGGAGGACAACCTCCCGGACCGCGATGCGCTGCGGGCTGCACTGGCCATCCAGCAACTGGCTGAGG GACTCACAGCTGATGACCTGCTGCTCGTGCTGATCTCAGGTGGTGAGCCTCATCCTGTCAGATGTGGTGG GGGACCCTGTGGAGGTGATTGCCAGTGGCCCCACCGTGGCCAGTTCCCACAATGTGCAAGATTGCCTGCA TATCCTCAATCGCTACGGCCTCCGTGCAGCCCTGCCACGTTCTGTGAAGACTGTGCTGTCTCGGGCCGAC TC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG227749 representing NM_001144951
Red=Cloning site Green=Tags(s) MAAALQVLPRLARAPLHPLLWRGSVARLASSMALAEQARQLFESAVGAVLPGPMLHRALSLDPGGRQLKV RDRNFQLRQNLYLVGFGKAVLGMAAAAEELLGQHLVQGVISVPKGIRAAMERAGKQEMLLKPHSRVQVFE GAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGEPHPVRCGGGPCGGDCQWPHRGQFPQCARLPA YPQSLRPPCSPATFCEDCAVSGRL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001144951 |
ORF Size | 702 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_001144951.1, NP_001138423.1 |
RefSeq Size | 1371 bp |
RefSeq ORF | 705 bp |
Locus ID | 132158 |
UniProt ID | Q8IVS8 |
Cytogenetics | 3p21.2 |
Protein Families | Transcription Factors |
Protein Pathways | Glycerolipid metabolism, Glycine, Glyoxylate and dicarboxylate metabolism, Metabolic pathways, serine and threonine metabolism |
Summary | This locus encodes a member of the glycerate kinase type-2 family. The encoded enzyme catalyzes the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. Decreased activity of the encoded enzyme may be associated with the disease D-glyceric aciduria. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
RC227749 | GLYCTK (Myc-DDK-tagged)-Human glycerate kinase (GLYCTK), transcript variant 2 | 10 ug |
$330.00
|
|
RC227749L3 | Lenti-ORF clone of GLYCTK (Myc-DDK-tagged)-Human glycerate kinase (GLYCTK), transcript variant 2 | 10 ug |
$630.00
|
|
RC227749L4 | Lenti-ORF clone of GLYCTK (mGFP-tagged)-Human glycerate kinase (GLYCTK), transcript variant 2 | 10 ug |
$630.00
|
|
SC325640 | GLYCTK (untagged)-Human glycerate kinase (GLYCTK), transcript variant 2 | 10 ug |
$300.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.