NCOA1 (NM_003743) Human Tagged ORF Clone
SKU
RG224812
NCOA1 (tGFP-tagged) - Human nuclear receptor coactivator 1 (NCOA1), transcript variant 1
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TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Type | Human Tagged ORF Clone |
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Target Symbol | NCOA1 |
Synonyms | bHLHe42; bHLHe74; F-SRC-1; KAT13A; RIP160; SRC1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RG224812 representing NM_003743
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCCTCGGGGACAGTTCATCCGACCCTGCTAACCCAGACTCACATAAGAGGAAAGGATCGCCAT GTGACACACTGGCATCAAGCACGGAAAAGAGGCGCAGGGAGCAAGAAAATAAATATTTAGAAGAACTAGC TGAGTTACTGTCTGCCAACATTAGTGACATTGACAGCTTGAGTGTAAAACCAGACAAATGCAAGATTTTG AAGAAAACAGTCGATCAGATACAGCTAATGAAGAGAATGGAACAAGAGAAATCAACAACTGATGACGATG TACAGAAATCAGACATCTCATCAAGTAGTCAAGGAGTGATAGAAAAGGAATCCTTGGGACCTCTTCTTTT GGAGGCTTTGGATGGATTTTTCTTTGTTGTGAACTGTGAAGGGAGAATTGTATTTGTGTCAGAGAATGTA ACCAGCTACTTAGGTTACAATCAGGAGGAATTAATGAATACCAGCGTCTACAGCATACTGCACGTGGGGG ATCATGCAGAATTTGTGAAGAATCTGCTACCAAAATCACTAGTAAATGGAGTTCCTTGGCCTCAAGAGGC AACACGACGAAATAGCCATACCTTTAACTGCAGGATGCTAATTCACCCTCCAGATGAGCCAGGGACCGAG AACCAAGAAGCTTGCCAGCGTTATGAAGTAATGCAGTGTTTCACTGTGTCACAGCCAAAATCAATTCAAG AGGATGGAGAAGATTTCCAGTCATGTCTGATTTGTATTGCACGGCGATTACCTCGGCCTCCAGCTATTAC GGGTGTAGAATCCTTTATGACCAAGCAAGATACTACAGGTAAAATCATCTCTATTGATACTAGTTCCCTG AGAGCTGCTGGCAGAACTGGTTGGGAAGATTTAGTGAGGAAGTGCATTTATGCTTTTTTCCAACCTCAGG GCAGAGAACCATCTTATGCCAGACAGCTGTTCCAAGAAGTGATGACTCGTGGCACTGCCTCCAGCCCCTC CTATAGATTCATATTGAATGATGGGACAATGCTTAGCGCCCACACCAAGTGTAAACTTTGCTACCCTCAA AGTCCAGACATGCAACCTTTCATCATGGGAATTCATATCATCGACAGGGAGCACAGTGGGCTTTCTCCTC AAGATGACACTAATTCTGGAATGTCAATTCCCCGAGTAAATCCCTCGGTCAATCCTAGTATCTCTCCAGC TCATGGTGTGGCTCGTTCATCCACATTGCCACCATCCAACAGCAACATGGTATCCACCAGAATAAACCGC CAGCAGAGCTCAGACCTTCATAGCAGCAGTCATAGTAATTCTAGCAACAGCCAAGGAAGTTTCGGATGCT CACCCGGAAGTCAGATTGTAGCCAATGTTGCCTTAAACCAAGGACAGGCCAGTTCACAGAGCAGTAATCC CTCTTTAAACCTCAATAATTCTCCTATGGAAGGTACAGGAATATCCCTAGCACAGTTCATGTCTCCAAGG AGACAGGTTACTTCTGGATTGGCAACAAGGCCCAGGATGCCAAACAATTCCTTTCCTCCTAATATTTCGA CATTAAGCTCTCCCGTTGGCATGACAAGTAGTGCCTGTAATAATAATAACCGATCTTATTCAAACATCCC AGTAACATCTTTACAGGGTATGAATGAAGGACCCAATAACTCCGTTGGCTTCTCTGCCAGTTCTCCAGTC CTCAGGCAGATGAGCTCACAGAATTCACCTAGCAGATTAAATATACAACCAGCAAAAGCTGAGTCCAAAG ATAACAAAGAGATTGCCTCAATTTTAAATGAAATGATTCAATCTGACAACAGCTCTAGTGATGGCAAACC TCTGGATTCAGGGCTTCTGCATAACAATGACAGACTTTCAGATGGAGACAGTAAATACTCTCAAACCAGT CACAAACTAGTGCAGCTTTTGACAACAACTGCCGAACAGCAGTTACGGCATGCTGATATAGACACAAGCT GCAAAGATGTCCTGTCTTGCACAGGCACTTCCAACTCTGCCTCTGCTAACTCTTCAGGAGGTTCTTGTCC CTCTTCTCATAGCTCATTGACAGAACGGCATAAAATTCTACACCGGCTCTTACAGGAGGGTAGCCCCTCA GATATCACCACTTTGTCTGTCGAGCCTGATAAAAAGGACAGTGCATCTACTTCTGTGTCAGTGACTGGAC AGGTACAAGGAAACTCCAGTATAAAACTAGAACTGGATGCTTCAAAGAAAAAAGAATCAAAAGACCATCA GCTCCTACGCTATCTTTTAGATAAAGATGAGAAAGATTTAAGATCAACTCCAAACCTGAGCCTGGATGAT GTAAAGGTGAAAGTGGAAAAGAAAGAACAGATGGATCCATGTAATACAAACCCAACCCCAATGACCAAAC CCACTCCTGAGGAAATAAAACTGGAGGCCCAGAGCCAGTTTACAGCTGACCTTGACCAGTTTGATCAGTT ACTGCCCACGCTGGAGAAGGCAGCACAGTTGCCAGGCTTATGTGAGACAGACAGGATGGATGGTGCGGTC ACCAGTGTAACCATCAAATCGGAGATCCTGCCAGCTTCACTTCAGTCCGCCACTGCCAGACCCACTTCCA GGCTAAATAGATTACCTGAGCTGGAATTGGAAGCAATTGATAACCAATTTGGACAACCAGGAACAGGCGA TCAGATTCCATGGACAAATAATACAGTGACAGCTATAAATCAGAGTAAATCAGAAGACCAGTGTATTAGC TCACAATTAGATGAGCTTCTCTGTCCACCCACAACAGTAGAAGGGAGAAATGATGAGAAGGCTCTTCTTG AACAGCTGGTATCCTTCCTTAGTGGCAAAGATGAAACTGAGCTAGCTGAACTAGACAGAGCTCTGGGAAT TGACAAACTTGTTCAGGGGGGTGGATTAGATGTATTATCAGAGAGATTTCCACCACAACAAGCAACGCCA CCTTTGATCATGGAAGAAAGACCCAACCTTTATTCCCAGCCTTACTCTTCTCCTTCTCCTACTGCCAATC TCCCTAGCCCTTTCCAAGGCATGGTCAGGCAAAAACCTTCACTGGGGACGATGCCTGTTCAAGTAACACC TCCCCGAGGTGCTTTTTCACCTGGCATGGGCATGCAGCCCAGGCAAACTCTAAACAGACCTCCGGCTGCA CCTAACCAGCTTCGACTTCAACTACAGCAGCGATTACAGGGACAACAGCAGTTGATACACCAAAATCGGC AAGCTATCTTAAACCAGTTTGCAGCAACTGCTCCTGTTGGCATCAATATGAGATCAGGCATGCAACAGCA AATTACACCTCAGCCACCCCTGAATGCTCAAATGTTGGCACAACGTCAGCGGGAACTGTACAGTCAACAG CACCGACAGAGGCAGCTAATACAGCAGCAAAGAGCCATGCTTATGAGGCAGCAAAGCTTTGGGAACAACC TCCCTCCCTCATCTGGACTACCAGTTCAAATGGGGAACCCCCGTCTTCCTCAGGGTGCTCCACAGCAATT CCCCTATCCACCAAACTATGGTACAAATCCAGGAACCCCACCTGCTTCTACCAGCCCGTTTTCACAACTA GCAGCAAATCCTGAAGCATCCTTGGCCAACCGCAACAGCATGGTGAGCAGAGGCATGACAGGAAACATAG GAGGACAGTTTGGCACTGGAATCAATCCTCAGATGCAGCAGAATGTCTTCCAGTATCCAGGAGCAGGAAT GGTTCCCCAAGGTGAGGCCAACTTTGCTCCATCTCTAAGCCCTGGGAGCTCCATGGTGCCGATGCCAATC CCTCCTCCTCAGAGTTCTCTGCTCCAGCAAACTCCACCTGCCTCCGGGTATCAGTCACCAGACATGAAGG CCTGGCAGCAAGGAGCGATAGGAAACAACAATGTGTTCAGTCAAGCTGTCCAGAACCAGCCCACGCCTGC ACAGCCAGGAGTATACAACAACATGAGCATCACCGTTTCCATGGCAGGTGGAAATACGAATGTTCAGAAC ATGAACCCAATGATGGCCCAGATGCAGATGAGCTCTTTGCAGATGCCAGGAATGAACACTGTGTGCCCTG AGCAGATAAATGATCCCGCACTGAGACACACAGGCCTCTACTGCAACCAGCTCTCATCCACTGACCTTCT CAAAACAGAAGCAGATGGAACCCAGCAGGTGCAACAGGTTCAGGTGTTTGCTGACGTCCAGTGTACAGTG AATCTGGTAGGCGGGGACCCTTACCTGAACCAGCCTGGTCCACTGGGAACTCAAAAGCCCACGTCAGGAC CACAGACCCCCCAGGCCCAGCAGAAGAGCCTCCTTCAGCAGCTACTGACTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG224812 representing NM_003743
Red=Cloning site Green=Tags(s) MSGLGDSSSDPANPDSHKRKGSPCDTLASSTEKRRREQENKYLEELAELLSANISDIDSLSVKPDKCKIL KKTVDQIQLMKRMEQEKSTTDDDVQKSDISSSSQGVIEKESLGPLLLEALDGFFFVVNCEGRIVFVSENV TSYLGYNQEELMNTSVYSILHVGDHAEFVKNLLPKSLVNGVPWPQEATRRNSHTFNCRMLIHPPDEPGTE NQEACQRYEVMQCFTVSQPKSIQEDGEDFQSCLICIARRLPRPPAITGVESFMTKQDTTGKIISIDTSSL RAAGRTGWEDLVRKCIYAFFQPQGREPSYARQLFQEVMTRGTASSPSYRFILNDGTMLSAHTKCKLCYPQ SPDMQPFIMGIHIIDREHSGLSPQDDTNSGMSIPRVNPSVNPSISPAHGVARSSTLPPSNSNMVSTRINR QQSSDLHSSSHSNSSNSQGSFGCSPGSQIVANVALNQGQASSQSSNPSLNLNNSPMEGTGISLAQFMSPR RQVTSGLATRPRMPNNSFPPNISTLSSPVGMTSSACNNNNRSYSNIPVTSLQGMNEGPNNSVGFSASSPV LRQMSSQNSPSRLNIQPAKAESKDNKEIASILNEMIQSDNSSSDGKPLDSGLLHNNDRLSDGDSKYSQTS HKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTERHKILHRLLQEGSPS DITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDHQLLRYLLDKDEKDLRSTPNLSLDD VKVKVEKKEQMDPCNTNPTPMTKPTPEEIKLEAQSQFTADLDQFDQLLPTLEKAAQLPGLCETDRMDGAV TSVTIKSEILPASLQSATARPTSRLNRLPELELEAIDNQFGQPGTGDQIPWTNNTVTAINQSKSEDQCIS SQLDELLCPPTTVEGRNDEKALLEQLVSFLSGKDETELAELDRALGIDKLVQGGGLDVLSERFPPQQATP PLIMEERPNLYSQPYSSPSPTANLPSPFQGMVRQKPSLGTMPVQVTPPRGAFSPGMGMQPRQTLNRPPAA PNQLRLQLQQRLQGQQQLIHQNRQAILNQFAATAPVGINMRSGMQQQITPQPPLNAQMLAQRQRELYSQQ HRQRQLIQQQRAMLMRQQSFGNNLPPSSGLPVQMGNPRLPQGAPQQFPYPPNYGTNPGTPPASTSPFSQL AANPEASLANRNSMVSRGMTGNIGGQFGTGINPQMQQNVFQYPGAGMVPQGEANFAPSLSPGSSMVPMPI PPPQSSLLQQTPPASGYQSPDMKAWQQGAIGNNNVFSQAVQNQPTPAQPGVYNNMSITVSMAGGNTNVQN MNPMMAQMQMSSLQMPGMNTVCPEQINDPALRHTGLYCNQLSSTDLLKTEADGTQQVQQVQVFADVQCTV NLVGGDPYLNQPGPLGTQKPTSGPQTPQAQQKSLLQQLLTE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003743 |
ORF Size | 4323 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_003743.4, NP_003734.3 |
RefSeq Size | 6895 bp |
RefSeq ORF | 4326 bp |
Locus ID | 8648 |
UniProt ID | Q15788 |
Cytogenetics | 2p23.3 |
Domains | HLH, PAS |
Protein Families | Druggable Genome, Transcription Factors |
Summary | The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
RC224812 | NCOA1 (Myc-DDK-tagged)-Human nuclear receptor coactivator 1 (NCOA1), transcript variant 1 | 10 ug |
$1,233.00
|
|
RC224812L1 | Lenti ORF clone of Human nuclear receptor coactivator 1 (NCOA1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,533.00
|
|
RC224812L2 | Lenti ORF clone of Human nuclear receptor coactivator 1 (NCOA1), transcript variant 1, mGFP tagged | 10 ug |
$1,533.00
|
|
RC224812L3 | Lenti ORF clone of Human nuclear receptor coactivator 1 (NCOA1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,533.00
|
|
RC224812L4 | Lenti ORF clone of Human nuclear receptor coactivator 1 (NCOA1), transcript variant 1, mGFP tagged | 10 ug |
$1,533.00
|
|
SC309911 | NCOA1 (untagged)-Human nuclear receptor coactivator 1 (NCOA1), transcript variant 1 | 10 ug |
$1,233.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.