Elastin (ELN) (NM_001081752) Human Tagged ORF Clone

SKU RG224648

ELN (tGFP-tagged) - Human elastin (ELN), transcript variant 2. Note: ORF is codon optimized

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Elastin
• Synonyms: ADCL1; SVAS; WBS; WS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224648 ORF sequence, codon optimized.; Due to the complexity of NM_001081752, the ORF clone is codon optimized for mammalian Expression. ; The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.; ; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGGCCTGACAGCCGCTGCCCCAAGGCCCGGCGTGCTGTTGCTTCTGCTGTCTATTCTGCACCCTT; CTAGACCTGGGGGGGTACCGGGGGCCATTCCCGGTGGCGTCCCAGGCGGGGTGTTTTACCCCGCACTGGG; CCCAGGGGGCAAGCCTCTGAAGCCTGTGCCCGGTGGCCTCGCTGGGGCTGGCCTCGGTGCGGGCTTGGGC; GCGTTTCCCGCTGTCACCTTCCCTGGTGCCCTGGTACCAGGCGGCGTGGCCGACGCTGCGGCCGCGTACA; AAGCCGCTAAGGCGGGAGCAGGACTCGGGGGAGTACCCGGGGTAGGTGGCCTCGGCGTAAGCGCTGGAGC; CGTCGTGCCTCAGCCTGGCGCCGGAGTCAAACCTGGGAAGGTGCCCGGGGTAGGGCTGCCTGGAGTGTAC; CCAGGCGGAGTTCTCCCCGGCGCCCGCTTCCCCGGGGTGGGAGTCCTGCCAGGGGTGCCAACAGGAGCCG; GAGTGAAACCCAAAGCACCTGGCGTCGGCGGTGCGTTCGCCGGTATCCCTGGGGTAGGTCCTTTCGGGGG; CCCTCAACCCGGTGTGCCGCTTGGTTATCCCATCAAAGCCCCAAAACTTCCAGGTGGGTACGGCTTGCCA; TATACAACAGGCAAGCTCCCCTATGGCTACGGACCGGGCGGGGTGGCCGGGGCAGCCGGCAAGGCTGGAT; ACCCCACCGGCACAGGAGTGGGGCCACAGGCGGCAGCGGCCGCCGCTGCCAAGGCAGCTGCCAAATTTGG; CGCGGGCGCCGCCGGAGTCCTCCCTGGGGTTGGCGGTGCCGGCGTACCTGGGGTACCCGGCGCCATCCCC; GGGATTGGAGGAATTGCCGGTGTCGGCACACCCGCAGCAGCCGCCGCCGCCGCCGCGGCGGCCAAGGCAG; CCAAATACGGTGCCGCTGCAGGGCTGGTACCTGGAGGACCAGGTTTTGGACCCGGAGTCGTCGGAGTTCC; CGGGGCGGGAGTCCCTGGCGTCGGGGTTCCCGGCGCCGGGATCCCCGTAGTCCCCGGAGCTGGAATTCCT; GGCGCCGCCGTGCCTGGAGTTGTGAGTCCTGAAGCAGCCGCAAAAGCTGCCGCCAAAGCTGCTAAGTATG; GCGCCCGCCCCGGCGTCGGAGTGGGAGGCATTCCTACATATGGCGTGGGAGCCGGCGGGTTCCCTGGATT; CGGCGTGGGTGTCGGCGGAATTCCCGGCGTGGCCGGCGTTCCTGGCGTTGGGGGAGTTCCCGGTGTTGGC; GGGGTTCCTGGCGTGGGGATCAGCCCAGAAGCCCAAGCCGCTGCCGCTGCGAAAGCCGCCAAGTATGGAC; TGGTTCCGGGAGTCGGGGTCGCACCGGGCGTTGGAGTTGCCCCTGGCGTGGGAGTGGCACCTGGGGTGGG; GCTGGCCCCCGGTGTGGGAGTTGCTCCCGGTGTAGGAGTGGCTCCAGGTGTGGGCGTAGCACCCGGCATT; GGTCCCGGGGGCGTGGCCGCGGCCGCCAAATCTGCGGCCAAGGTTGCCGCCAAAGCACAATTGAGGGCAG; CCGCTGGTTTGGGAGCTGGTATCCCTGGGCTGGGTGTGGGAGTGGGAGTACCCGGTCTTGGTGTGGGAGC; GGGGGTTCCAGGACTCGGCGTGGGCGCCGGCGTCCCTGGATTTGGTGCAGTGCCGGGTGCCCTCGCAGCT; GCAAAAGCGGCTAAATACGGGGCGGCAGTCCCTGGAGTGCTGGGGGGCCTTGGCGCCTTGGGAGGCGTCG; GAATACCTGGAGGGGTTGTGGGCGCTGGACCAGCTGCCGCAGCCGCAGCCGCCAAGGCCGCTGCTAAGGC; TGCCCAGTTCGGCCTCGTGGGGGCCGCCGGATTGGGAGGGCTTGGCGTCGGTGGCCTCGGAGTGCCCGGG; GTGGGAGGGCTCGGTGGCATTCCTCCCGCTGCAGCAGCAAAAGCCGCCAAATACGGAGTGGCAGCCAGAC; CTGGGTTCGGCCTGAGTCCAATCTTTCCAGGAGGCGCTTGCCTCGGTAAAGCTTGCGGAAGAAAGCGCAA; G; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224648 representing NM_001081752; Red=Cloning site Green=Tags(s); ; MAGLTAAAPRPGVLLLLLSILHPSRPGGVPGAIPGGVPGGVFYPALGPGGKPLKPVPGGLAGAGLGAGLG; AFPAVTFPGALVPGGVADAAAAYKAAKAGAGLGGVPGVGGLGVSAGAVVPQPGAGVKPGKVPGVGLPGVY; PGGVLPGARFPGVGVLPGVPTGAGVKPKAPGVGGAFAGIPGVGPFGGPQPGVPLGYPIKAPKLPGGYGLP; YTTGKLPYGYGPGGVAGAAGKAGYPTGTGVGPQAAAAAAAKAAAKFGAGAAGVLPGVGGAGVPGVPGAIP; GIGGIAGVGTPAAAAAAAAAAKAAKYGAAAGLVPGGPGFGPGVVGVPGAGVPGVGVPGAGIPVVPGAGIP; GAAVPGVVSPEAAAKAAAKAAKYGARPGVGVGGIPTYGVGAGGFPGFGVGVGGIPGVAGVPGVGGVPGVG; GVPGVGISPEAQAAAAAKAAKYGLVPGVGVAPGVGVAPGVGVAPGVGLAPGVGVAPGVGVAPGVGVAPGI; GPGGVAAAAKSAAKVAAKAQLRAAAGLGAGIPGLGVGVGVPGLGVGAGVPGLGVGAGVPGFGAVPGALAA; AKAAKYGAAVPGVLGGLGALGGVGIPGGVVGAGPAAAAAAAKAAAKAAQFGLVGAAGLGGLGVGGLGVPG; VGGLGGIPPAAAAKAAKYGVAARPGFGLSPIFPGGACLGKACGRKRK; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081752
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001081752.1, NP_001075221.1
• RefSeq Size: 3648 bp
• RefSeq ORF: 2034 bp
• Locus ID: 2006
• UniProt ID: P15502
• Cytogenetics: 7q11.23
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Summary: This gene encodes a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Degradation products of the encoded protein, known as elastin-derived peptides or elastokines, bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. [provided by RefSeq, Aug 2017]