BBS7 (NM_176824) Human Tagged ORF Clone

SKU RG224479

BBS7 (tGFP-tagged) - Human Bardet-Biedl syndrome 7 (BBS7), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: BBS7
• Synonyms: BBS2L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224479 representing NM_176824; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATCTGATTTTAAACCGAATGGATTATCTGCAGGTGGGAGTAACATCTCAGAAGACTATGAAGCTAA; TTCCTGCCTCAAGACACAGAGCTACACAAAAGGTGGTTATTGGAGATCATGATGGGGTAGTTATGTGCTT; TGGCATGAAGAAAGGAGAAGCAGCAGCAGTGTTCAAGACTTTACCCGGGCCGAAGATTGCAAGGCTGGAA; CTGGGAGGGGTTATCAACACACCTCAGGAGAAAATTTTTATTGCTGCAGCATCTGAGATTAGAGGCTTCA; CAAAAAGAGGAAAACAGTTCCTCTCCTTTGAAACAAACCTCACTGAAAGCATTAAAGCTATGCACATATC; TGGCTCAGACCTCTTTCTCAGTGCAAGTTACATCTATAACCATTATTGTGACTGCAAAGACCAACATTAT; TACCTTTCTGGGGATAAAATCAATGATGTGATCTGCCTTCCAGTGGAAAGATTATCTCGTATCACACCTG; TATTGGCCTGCCAGGACAGAGTGCTCAGAGTTTTACAGGGATCTGATGTGATGTATGCAGTTGAAGTTCC; TGGACCCCCTACTGTCTTAGCACTACACAATGGAAATGGCGGTGACTCTGGAGAAGACCTTTTGTTTGGG; ACATCAGACGGAAAACTTGCGCTTATACAGATTACTACATCCAAACCAGTACGCAAGTGGGAAATTCAAA; ATGAGAAAAAGAGAGGAGGTATTTTGTGTATTGACAGCTTTGACATTGTGGGTGATGGGGTTAAAGATTT; ACTTGTTGGGAGAGATGACGGAATGGTGGAAGTGTATAGTTTTGATAATGCAAATGAACCTGTTCTACGA; TTTGATCAGATGTTGTCTGAAAGCGTCACATCTATCCAGGGTGGTTGTGTAGGAAAAGACAGCTATGATG; AAATCGTGGTGTCCACATATTCAGGCTGGGTTACAGGTCTGACAACAGAGCCCATTCATAAGGAAAGTGG; ACCAGGAGAAGAACTAAAAATTAATCAGGAGATGCAGAATAAAATTTCTTCCTTACGGAATGAGTTGGAA; CATTTGCAGTATAAGGTATTGCAGGAAAGAGAGAATTATCAACAGTCTTCTCAATCAAGCAAAGCAAAAT; CAGCAGTACCTTCCTTTGGTATAAATGATAAATTTACACTAAATAAAGATGATGCCAGTTACAGCCTTAT; CTTAGAGGTACAGACTGCAATAGATAATGTCTTAATACAGAGTGATGTTCCAATAGATTTACTTGATGTG; GATAAAAATTCTGCTGTTGTTAGCTTTAGCAGCTGTGATTCTGAGTCAAACGACAACTTCCTTCTTGCCA; CTTATCGGTGCCAGGCAGATACTACAAGGCTGGAACTCAAGATTCGCTCAATTGAAGGCCAGTATGGCAC; ACTACAAGCATATGTGACTCCAAGAATTCAACCCAAAACCTGTCAGGTCCGCCAGTACCACATCAAACCT; CTTTCACTCCATCAAAGAACTCACTTTATTGATCATGACAGACCCATGAATACACTGACCCTAACAGGCC; AGTTCAGTTTTGCTGAAGTTCACTCCTGGGTGGTTTTTTGTCTGCCTGAAGTTCCAGAAAAACCTCCAGC; AGGAGAATGTGTGACATTTTACTTTCAGAACACCTTTCTAGATACACAACTTGAAAGTACCTACAGAAAA; GGAGAGGGAGTTTTTAAATCTGACAACATTTCTACTATCTCCATCCTAAAAGATGTGCTTTCTAAAGAAG; CTACAAAAAGGAAAATTAACCTCAACATATCATACGAGATAAATGAAGTATCAGTCAAACACACTTTAAA; GCTAATCCACCCAAAGCTGGAGTACCAGTTGCTTTTGGCTAAGAAAGTGCAGTTAATTGATGCTTTAAAA; GAATTACAGATTCATGAGGGAAATACGAACTTTCTGATACCAGAATATCACTGTATTCTAGAAGAGGCAG; ATCACCTACAGGAAGAATACAAAAAGCAACCTGCACATCTTGAAAGACTCTATGGCATGATCACTGATCT; TTTCATAGATAAATTTAAGTTTAAAGGCACCAATGTAAAAACTAAAGTACCCCTTCTATTGGAAATTCTG; GACAGTTATGACCAAAATGCATTGATTTCATTCTTCGATGCAGCA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224479 representing NM_176824; Red=Cloning site Green=Tags(s); ; MDLILNRMDYLQVGVTSQKTMKLIPASRHRATQKVVIGDHDGVVMCFGMKKGEAAAVFKTLPGPKIARLE; LGGVINTPQEKIFIAAASEIRGFTKRGKQFLSFETNLTESIKAMHISGSDLFLSASYIYNHYCDCKDQHY; YLSGDKINDVICLPVERLSRITPVLACQDRVLRVLQGSDVMYAVEVPGPPTVLALHNGNGGDSGEDLLFG; TSDGKLALIQITTSKPVRKWEIQNEKKRGGILCIDSFDIVGDGVKDLLVGRDDGMVEVYSFDNANEPVLR; FDQMLSESVTSIQGGCVGKDSYDEIVVSTYSGWVTGLTTEPIHKESGPGEELKINQEMQNKISSLRNELE; HLQYKVLQERENYQQSSQSSKAKSAVPSFGINDKFTLNKDDASYSLILEVQTAIDNVLIQSDVPIDLLDV; DKNSAVVSFSSCDSESNDNFLLATYRCQADTTRLELKIRSIEGQYGTLQAYVTPRIQPKTCQVRQYHIKP; LSLHQRTHFIDHDRPMNTLTLTGQFSFAEVHSWVVFCLPEVPEKPPAGECVTFYFQNTFLDTQLESTYRK; GEGVFKSDNISTISILKDVLSKEATKRKINLNISYEINEVSVKHTLKLIHPKLEYQLLLAKKVQLIDALK; ELQIHEGNTNFLIPEYHCILEEADHLQEEYKKQPAHLERLYGMITDLFIDKFKFKGTNVKTKVPLLLEIL; DSYDQNALISFFDAA; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176824
• ORF Size: 2145 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_176824.3
• RefSeq Size: 3718 bp
• RefSeq ORF: 2148 bp
• Locus ID: 55212
• UniProt ID: Q8IWZ6
• Cytogenetics: 4q27
• Summary: This gene encodes one of eight proteins that form the BBSome complex containing BBS1, BBS2, BBS4, BBS5, BBS7, BBS8, BBS9 and BBIP10. The BBSome complex is believed to recruit Rab8(GTP) to the primary cilium and promote ciliogenesis. The BBSome complex assembly is mediated by a complex composed of three chaperonin-like BBS proteins (BBS6, BBS10, and BBS12) and CCT/TRiC family chaperonins. Mutations in this gene are implicated in Bardet-Biedl syndrome, a genetic disorder whose symptoms include obesity, retinal degeneration, polydactyly and nephropathy; however, mutations in this gene and the BBS8 gene are thought to play a minor role and mutations in chaperonin-like BBS genes are found to be a major contributor to disease development in a multiethnic Bardet-Biedl syndrome patient population. Two transcript variants encoding distinct isoforms have been identified for this gene.[provided by RefSeq, Oct 2014]