ZNF257 (NM_033468) Human Tagged ORF Clone

SKU
RG223565
ZNF257 (tGFP-tagged) - Human zinc finger protein 257 (ZNF257). Note: ORF is codon optimized
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    Expression-ready ORF plasmid with C-terminal tag(s)

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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF257
Synonyms BMZF-4; BMZF4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG223565 ORF sequence, codon optimized.
Due to the complexity of NM_033468, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical
.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACCCCTTACCATCCGGGATGTGACAGTTGAATTCTCACTGGAAGAATGGCATTGTCTCGATACTG
CCCAGCAGAACCTGTATCGAGATGTCATGTTGGAGAACTACCGAAATCTGGTTTTTCTGGGTATTGCAGT
GAGCAAACCTGATCTCATTACATGCTTGGAGCAGGGCAAGGAGCCGTGTAATATGAAAAGACACGAAATG
GTTGCAAAACCACCAGTCATGTGTTCACACATCGCGGAGGACCTTTGCCCAGAGCGCGACATTAAGTATT
TCTTCCAGAAGGTAATACTGAGGAGATATGACAAATGTGAACACGAAAACCTCCAGCTCAGGAAGGGATG
CAAGAGTGTGGATGAATGTAAGGTCTGCAAGGGCGGTTACAACGGCCTGAACCAATGTCTTATTACGACT
CAGAGTAAAATGTATCAGTGTGACAAATACGTCAAAGTCTTTTACAAGTTTAGCAATTCCGACCGCCACA
AAATAAGGCACACTGAAAAGAAGACCTGTAAATGTAAGGAATGTGGCAAAAGCTTCTGTATGCTCAGCCA
GCTTACCAGGCATAAACGAATACATATTCGAGAGAATAGTCACAAGTGCGAGGAATGTGGAAAGGCTTTT
AATCAGAGTTCCGCATTGACACGGCACAAGATGACACATACAGGTGAGAAGCCGTATAAGTGTGAGGAGT
GCGGGAAGGCATTTAACCGCTCCTCTCACCTGACTCAACACAAAGTTATCCACACACGAGAAAAGCCGTA
TAAGTGCGAAGAATGTGGCAAGGCCTTCAATAGGAGCTCTCACATTACCCAACATAAGCGGATCCATAAT
CGGGAGAAGCCCTTTAAATATGATGAGTGCTGCAAGGCCTTCAAGTGGAGTTCAGCTCTGACTACACTGA
CACAGCACAAGCGGATCCATACCGGGGAGAAGCCATACAAATGCGAAGAATGCGGTAAGGCATTCAATCA
GTCTTCAGCTCTGACCAGGCACAAGATGATCCACACCGGAGAGAAACCTTTCCAGTGTGAGGAGTGTGGG
AAGGCATTTAATCGAAGTAGCCACCTGACTCAGCACAAAATTATCCATACAAAAGAAAAACCGTACAAGT
GTGAAGAGTGCGGGAAGGCTTTTAACCGAAGCAGTCACTTGACGAAGCATAAGAGAATACACACCAGAGA
GAAAGCATATAAATGCGACGAATATTGTAAGGCCTTCAATTGGTCTTCCGCACTGACCACCCTTACCCAG
CACAAGATCATCCATACAGGTGAGAAGCCCTACAAGTGCGAGGAATGTGGCAAAGCATTTAACCGGTCTT
CTTACCTGATTAGACATAAAATCATCCACACAGGGGAGAAGCCCTACAAGTGCGAAGAGTGCGGCAAGGC
CTTCAATCAGTCCAGCCACCTCACGCAGCATAAGATCATCCACACAGGAGAAAAGCCATACAAGTGTGAA
GAATGTGGAAAAGCTTTCAATCGGAGCTCACATCTCTCACAGCACAAGATCATCCATACGGGGGAGAAGC
CTTATAAGTGTGAGGAGTGTGGAAAGCCCTTTAACCGATTCTCCTACCTGACCGTCCATAAAAGGATCCA
TGCCGGAGAAAACCCCAACAAGTATGAGGAATGTGGGAAAGCCTGCAATCATTCTTCCAACCTTACTAAA
CATAATAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG223565 representing NM_033468
Red=Cloning site Green=Tags(s)

MGPLTIRDVTVEFSLEEWHCLDTAQQNLYRDVMLENYRNLVFLGIAVSKPDLITCLEQGKEPCNMKRHEM
VAKPPVMCSHIAEDLCPERDIKYFFQKVILRRYDKCEHENLQLRKGCKSVDECKVCKGGYNGLNQCLITT
QSKMYQCDKYVKVFYKFSNSDRHKIRHTEKKTCKCKECGKSFCMLSQLTRHKRIHIRENSHKCEECGKAF
NQSSALTRHKMTHTGEKPYKCEECGKAFNRSSHLTQHKVIHTREKPYKCEECGKAFNRSSHITQHKRIHN
REKPFKYDECCKAFKWSSALTTLTQHKRIHTGEKPYKCEECGKAFNQSSALTRHKMIHTGEKPFQCEECG
KAFNRSSHLTQHKIIHTKEKPYKCEECGKAFNRSSHLTKHKRIHTREKAYKCDEYCKAFNWSSALTTLTQ
HKIIHTGEKPYKCEECGKAFNRSSYLIRHKIIHTGEKPYKCEECGKAFNQSSHLTQHKIIHTGEKPYKCE
ECGKAFNRSSHLSQHKIIHTGEKPYKCEECGKPFNRFSYLTVHKRIHAGENPNKYEECGKACNHSSNLTK
HNS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_033468
ORF Size 1689 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033468.2, NP_258429.2
RefSeq Size 3550 bp
RefSeq ORF 1692 bp
Locus ID 113835
UniProt ID Q9Y2Q1
Cytogenetics 19p12
Domains KRAB, zf-C2H2
Protein Families Transcription Factors
Summary May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RC223565 ZNF257 (Myc-DDK-tagged)-Human zinc finger protein 257 (ZNF257). Note: ORF is codon optimized 10 ug
$289.00 MSRP $524.00 MSRP $524.00
SC317967 ZNF257 (untagged)-Human zinc finger protein 257 (ZNF257) 10 ug
$577.00

Citations

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