ADAM17 (NM_003183) Human Tagged ORF Clone

SKU RG222457

ADAM17 (tGFP-tagged) - Human ADAM metallopeptidase domain 17 (ADAM17). Note: ORF is codon optimized

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ADAM17
• Synonyms: ADAM18; CD156B; CSVP; NISBD; NISBD1; TACE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222457 ORF sequence, codon optimized.; Due to the complexity of NM_003183, the ORF clone is codon optimized for mammalian Expression. ; The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.; ; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCGACAGTCCCTCCTCTTCCTTACCAGTGTGGTACCATTCGTGCTTGCCCCTAGACCCCCAGACGACC; CAGGCTTCGGACCTCATCAAAGACTGGAGAAACTCGACTCACTTCTCTCCGACTATGATATCCTGAGCCT; TAGCAATATCCAACAACACTCCGTGAGGAAGCGGGACCTGCAGACTTCAACTCATGTGGAAACACTGCTG; ACGTTTTCCGCACTGAAGAGGCATTTCAAGCTCTACCTTACATCTAGCACTGAGCGCTTCTCTCAAAACT; TTAAGGTGGTGGTTGTAGACGGAAAAAACGAGAGCGAATATACAGTCAAGTGGCAAGATTTTTTCACGGG; CCACGTTGTCGGCGAACCAGATAGCAGGGTGCTGGCGCATATTCGCGACGACGACGTGATAATCAGAATC; AACACCGACGGAGCTGAGTACAACATCGAGCCCCTTTGGCGGTTCGTGAATGACACAAAAGACAAGCGGA; TGCTTGTATACAAGAGCGAGGACATCAAAAATGTGTCAAGGCTCCAGTCCCCCAAGGTGTGCGGGTACCT; GAAGGTGGATAATGAGGAGCTTCTGCCTAAAGGCCTCGTTGACCGAGAACCCCCGGAGGAACTGGTACAC; CGGGTAAAAAGGCGGGCAGACCCAGATCCCATGAAAAATACCTGTAAGCTGCTGGTCGTGGCCGATCATA; GATTCTACAGGTACATGGGTAGAGGCGAGGAATCTACAACAACAAACTACCTGATCGAACTGATTGATCG; CGTGGATGACATCTATAGGAATACGAGCTGGGACAACGCTGGCTTCAAGGGATACGGGATTCAGATTGAG; CAGATCCGAATACTGAAATCACCACAGGAAGTGAAACCTGGAGAGAAGCATTACAACATGGCGAAGTCCT; ATCCCAACGAGGAAAAAGATGCTTGGGATGTAAAGATGTTGCTCGAGCAGTTCTCTTTCGATATAGCTGA; GGAAGCAAGCAAAGTCTGCCTCGCCCACCTGTTTACCTACCAAGATTTTGACATGGGTACCTTGGGTCTT; GCCTACGTTGGCTCACCGCGCGCGAATAGCCACGGCGGCGTTTGCCCCAAGGCGTATTACTCACCCGTTG; GTAAGAAGAACATTTACCTCAACTCAGGGCTTACTTCCACTAAAAACTATGGAAAAACCATCCTTACCAA; GGAGGCTGACCTTGTTACGACCCACGAGCTTGGCCATAATTTCGGGGCTGAGCACGACCCAGACGGCTTG; GCTGAGTGTGCCCCAAATGAGGACCAGGGCGGAAAATATGTCATGTATCCAATCGCGGTCAGTGGCGACC; ATGAAAACAACAAAATGTTTTCAAATTGCTCCAAACAAAGTATCTACAAAACCATTGAATCTAAAGCGCA; GGAGTGCTTTCAAGAGAGGTCAAATAAAGTTTGTGGTAATTCCCGGGTGGACGAGGGAGAAGAATGCGAT; CCAGGGATCATGTATCTCAATAATGATACTTGTTGTAACTCCGACTGCACGCTGAAGGAAGGGGTGCAAT; GCTCCGACCGGAATAGTCCCTGTTGCAAGAATTGCCAGTTCGAGACTGCGCAGAAAAAGTGCCAGGAGGC; TATTAATGCAACCTGCAAAGGTGTCTCCTATTGTACGGGGAACTCATCTGAGTGTCCACCCCCTGGCAAT; GCCGAGGATGATACTGTGTGTTTGGATCTTGGTAAGTGCAAGGATGGGAAGTGTATCCCATTCTGCGAGC; GGGAGCAACAGTTGGAATCATGCGCCTGCAATGAGACTGATAATTCTTGCAAAGTCTGCTGTCGCGACCT; CAGTGGCCGCTGTGTCCCGTACGTAGATGCCGAACAAAAGAACTTGTTCCTGCGAAAGGGAAAACCTTGC; ACCGTGGGGTTCTGCGATATGAACGGAAAATGTGAAAAAAGAGTGCAAGACGTCATCGAGAGATTTTGGG; ATTTCATTGACCAGCTGAGCATTAATACATTTGGTAAGTTTCTCGCAGATAACATCGTGGGATCCGTCCT; CGTCTTTAGCTTGATTTTCTGGATCCCGTTCAGTATTCTTGTCCACTGTGTGGATAAGAAACTTGACAAG; CAGTATGAGTCTTTGAGCCTGTTTCATCCATCCAACGTGGAAATGCTGAGCAGTATGGATAGCGCATCAG; TGAGGATCATTAAGCCTTTCCCAGCACCTCAAACTCCTGGCCGCCTGCAGCCAGCGCCAGTGATCCCTTC; CGCGCCTGCTGCTCCAAAACTGGATCACCAAAGAATGGACACTATCCAGGAAGACCCCTCTACCGATAGC; CATATGGATGAAGACGGTTTCGAAAAGGACCCATTCCCGAACTCATCTACTGCTGCAAAATCCTTCGAAG; ATCTCACAGATCACCCAGTTACTAGATCCGAGAAGGCTGCAAGTTTTAAGCTCCAGCGCCAGAACAGGGT; CGATTCTAAGGAAACTGAGTGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222457 representing NM_003183; Red=Cloning site Green=Tags(s); ; MRQSLLFLTSVVPFVLAPRPPDDPGFGPHQRLEKLDSLLSDYDILSLSNIQQHSVRKRDLQTSTHVETLL; TFSALKRHFKLYLTSSTERFSQNFKVVVVDGKNESEYTVKWQDFFTGHVVGEPDSRVLAHIRDDDVIIRI; NTDGAEYNIEPLWRFVNDTKDKRMLVYKSEDIKNVSRLQSPKVCGYLKVDNEELLPKGLVDREPPEELVH; RVKRRADPDPMKNTCKLLVVADHRFYRYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGIQIE; QIRILKSPQEVKPGEKHYNMAKSYPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGL; AYVGSPRANSHGGVCPKAYYSPVGKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGL; AECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECD; PGIMYLNNDTCCNSDCTLKEGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGN; AEDDTVCLDLGKCKDGKCIPFCEREQQLESCACNETDNSCKVCCRDLSGRCVPYVDAEQKNLFLRKGKPC; TVGFCDMNGKCEKRVQDVIERFWDFIDQLSINTFGKFLADNIVGSVLVFSLIFWIPFSILVHCVDKKLDK; QYESLSLFHPSNVEMLSSMDSASVRIIKPFPAPQTPGRLQPAPVIPSAPAAPKLDHQRMDTIQEDPSTDS; HMDEDGFEKDPFPNSSTAAKSFEDLTDHPVTRSEKAASFKLQRQNRVDSKETEC; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003183
• ORF Size: 2472 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003183.4, NP_003174.3
• RefSeq Size: 4391 bp
• RefSeq ORF: 2475 bp
• Locus ID: 6868
• UniProt ID: P78536
• Cytogenetics: 2p25.1
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease, Epithelial cell signaling in Helicobacter pylori infection, Notch signaling pathway
• Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands, and plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. Additionally, this protease may play a role in viral infection through its cleavage of ACE2, the cellular receptor for SARS-CoV and SARS-CoV-2. [provided by RefSeq, Aug 2020]