PRDM10 (NM_020228) Human Tagged ORF Clone

SKU RG221532

PRDM10 (tGFP-tagged) - Human PR domain containing 10 (PRDM10), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PRDM10
• Synonyms: PFM7; TRIS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221532 representing NM_020228; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATTCCAAAGATGAAAGCTCGCATGTGTGGCCGACATCTGCAGAGCATGAACAGAATGCCGCACAGG; TGCACTTTGTTCCGGACACAGGAACAGTGGCTCAGATTGTCTATACCGATGACCAGGTTCGCCCCCCACA; GCAGGTGGTGTACACGGCAGATGGTGCCTCCTACACATCAGTGGATGGTCCAGAGCACACGCTGGTGTAC; ATCCACCCGGTGGAAGCTGCACAGACTCTGTTTACAGACCCCGGCCAGGTAGCTTATGTCCAACAGGATG; CTACAGCTCAGCAGGCCTCATTGCCAGTTCATAACCAGGTGCTGCCTTCCATCGAGAGTGTAGATGGGTC; CGACCCTTTGGCAACTCTGCAGACCCCTCTAGGCAGACTGGAGGCCAAAGAGGAAGAGGATGAGGATGAG; GACGAGGACACTGAGGAAGATGAGGAAGAAGACGGTGAGGACACGGATCTGGATGACTGGGAGCCAGACC; CGCCCCGGCCCTTCGACCCACACGACTTGTGGTGTGAGGAGTGCAATAACGCGCATGCTTCAGTGTGTCC; GAAGCACGGCCCCTTGCACCCGATCCCCAACCGGCCGGTGCTCACCCGGGCCAGGGCGAGCCTCCCCCTG; GTGCTCTACATAGACAGGTTTCTGGGCGGGGTGTTCTCCAAGCGGCGCATCCCCAAGCGCACCCAGTTTG; GCCCCGTGGAGGGGCCTCTCGTCAGGGGCTCGGAGCTGAAAGACTGTTACATTCACCTCAAGGTTTCTCT; TGATAAAGGGGACAGGAAAGAAAGGGATTTACATGAAGACCTATGGTTTGAGTTGTCTGATGAGACGCTT; TGTAACTGGATGATGTTTGTACGGCCAGCCCAGAATCACCTGGAGCAGAACCTGGTGGCTTACCAGTATG; GCCACCATGTGTATTATACAACCATAAAAAATGTGGAGCCCAAGCAGGAACTGAAGGTGTGGTATGCCGC; ATCCTATGCTGAGTTCGTGAACCAGAAAATTCATGACATTTCTGAGGAAGAAAGGAAAGTTCTTCGAGAG; CAAGAGAAGAATTGGCCCTGCTATGAATGTAACCGCCGATTTATAAGCTCGGAGCAGTTGCAACAGCATC; TCAATTCTCATGATGAGAAACTAGATGTGTTTAGCAGAACAAGAGGCAGAGGAAGGGGACGAGGCAAGAG; GCGATTCGGTCCAGGTCGACGGCCGGGGCGTCCTCCAAAATTTATCCGCCTGGAAATCACCAGCGAAAAT; GGGGAAAAGAGTGACGATGGGACACAGGACTTGCTACATTTTCCCACAAAGGAGCAATTTGATGAGGCTG; AACCAGCCACTCTGAATGGGCTGGATCAACCAGAACAGACCACTATCCCAATCCCTCAGCTGCCACAGGA; AACCCAGTCTTCCCTGGAACATGAACCAGAAACTCACACCCTGCACCTGCAGCCGCAGCATGAAGAGAGC; GTGGTGCCCACCCAGAGCACGCTGACAGCCGACGACATGCGCAGAGCCAAGCGCATCCGATTGGAGCTGC; AGAATGCAGCTCTTCAGCATCTGTTTATTCGGAAGTCCTTCCGGCCTTTTAAATGCTTGCAGTGTGGGAA; GGCCTTCCGGGAAAAGGACAAACTGGACCAGCACTTACGCTTCCATGGGCGGGAGGGGAACTGCCCACTG; ACCTGTGATCTCTGTAACAAGGGCTTCATCAGCAGCACATCCTTGGAGAGCCACATGAAGCTCCACTCAG; ACCAGAAGACTTACTCTTGCATTTTTTGCCCAGAATCCTTTGACCGCCTTGATTTGTTGAAAGATCATGT; GGCCATTCATATCAATGATGGCTACTTCACCTGCCCAACTTGTAAGAAACGGTTCCCAGATTTTATCCAG; GTGAAAAAACACGTGCGCAGCTTCCACTCAGAAAAGATCTACCAGTGCACAGAGTGTGACAAGGCCTTCT; GTCGCCCCGATAAACTGCGACTCCACATGCTCCGGCATTCGGACCGCAAAGACTTCCTGTGTTCCACCTG; TGGGAAGCAATTTAAGCGAAAAGACAAACTACGGGAACACATGCAGAGGATGCATAATCCTGAGAGGGAG; GCCAAGAAAGCCGACCGCATCAGCCGCTCCAAGACGTTCAAGCCCCGCATCACGTCCACAGACTACGACA; GCTTCACGTTCAAGTGCCGCCTGTGCATGATGGGCTTCCGGCGGCGCGGCATGCTGGTAAATCACTTATC; GAAGAGACACCCAGACATGAAGATAGAAGAGGTGCCAGAGTTAACTCTACCCATCATAAAACCCAATCGT; GATTACTTTTGTCAGTATTGCGATAAGGTTTATAAAAGTGCCAGCAAGCGCAAAGCCCACATTCTGAAGA; ACCACCCAGGAGCAGAGCTCCCACCGAGCATTCGGAAGCTCCGACCCGCTGGTCCTGGAGAGCCAGACCC; CATGCTGAGCACACACACCCAGCTGACGGGCACCATCGCCACCCCTCCCGTCTGCTGTCCCCACTGCTCC; AAGCAGTACAGCAGCAAGACCAAGATGGTCCAGCACATTCGAAAGAAGCATCCAGAGTTCGCCCAGCTCT; CCAACACCATACACACACCACTGACGACAGCTGTGATCAGTGCCACCCCAGCGGTTTTGACTACAGACAG; CGCCACTGGAGAGACTGTGGTGACGACGGACCTGCTCACCCAAGCAATGACAGAACTGTCCCAGACCTTA; ACGACAGACTACCGAACGCCACAAGGGGATTACCAGAGAATTCAGTACATCCCTGTGTCGCAGTCGGCGT; CTGGCCTCCAGCAGCCTCAGCACATACAGCTGCAAGTGGTTCAAGTGGCCTCGGCCACTTCCCCTCACCA; GTCACAGCAGTCCACTGTGGATGTTGGCCAGCTCCATGATCCTCAGCCCTACCCCCAGCACGCCATCCAG; GTGCAGCACATCCAGGTCAGCGAGCCTACCGCCTCGGCCCCGTCCTCCGCCCAGGTATCTGGGCAGCCGT; TGAGTCCCTCAGCCCAGCAGGCTCAGCAGGGGCTCAGCCCCTCCCACATCCAGGGCAGTTCTTCCACACA; GGGGCAGGCTCTGCAGCAGCAGCAGCAGCAGCAGCAGAATTCCTCTGTGCAGCACACGTACCTGCCCAGT; GCTTGGAATTCCTTCCGTGGCTATTCATCTGAGATTCAAATGATGACGCTTCCTCCGGGTCAGTTTGTGA; TTACAGACAGTGGTGTGGCAACTCCAGTTACTACTGGCCAGGTGAAGGCGGTTACTTCGGGTCATTATGT; GTTATCAGAAAGTCAATCAGAATTGGAAGAAAAGCAAACTTCTGCCCTCTCTGGTGGAGTCCAGGTCGAG; CCACCTGCACACAGTGACTCCCTGGACCCCCAGACCAACAGCCAACAGCAGACCACACAGTACATCATCA; CCACCACCACCAACGGGAACGGAAGCAGCGAAGTGCATATCACCAAACCA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221532 representing NM_020228; Red=Cloning site Green=Tags(s); ; MDSKDESSHVWPTSAEHEQNAAQVHFVPDTGTVAQIVYTDDQVRPPQQVVYTADGASYTSVDGPEHTLVY; IHPVEAAQTLFTDPGQVAYVQQDATAQQASLPVHNQVLPSIESVDGSDPLATLQTPLGRLEAKEEEDEDE; DEDTEEDEEEDGEDTDLDDWEPDPPRPFDPHDLWCEECNNAHASVCPKHGPLHPIPNRPVLTRARASLPL; VLYIDRFLGGVFSKRRIPKRTQFGPVEGPLVRGSELKDCYIHLKVSLDKGDRKERDLHEDLWFELSDETL; CNWMMFVRPAQNHLEQNLVAYQYGHHVYYTTIKNVEPKQELKVWYAASYAEFVNQKIHDISEEERKVLRE; QEKNWPCYECNRRFISSEQLQQHLNSHDEKLDVFSRTRGRGRGRGKRRFGPGRRPGRPPKFIRLEITSEN; GEKSDDGTQDLLHFPTKEQFDEAEPATLNGLDQPEQTTIPIPQLPQETQSSLEHEPETHTLHLQPQHEES; VVPTQSTLTADDMRRAKRIRLELQNAALQHLFIRKSFRPFKCLQCGKAFREKDKLDQHLRFHGREGNCPL; TCDLCNKGFISSTSLESHMKLHSDQKTYSCIFCPESFDRLDLLKDHVAIHINDGYFTCPTCKKRFPDFIQ; VKKHVRSFHSEKIYQCTECDKAFCRPDKLRLHMLRHSDRKDFLCSTCGKQFKRKDKLREHMQRMHNPERE; AKKADRISRSKTFKPRITSTDYDSFTFKCRLCMMGFRRRGMLVNHLSKRHPDMKIEEVPELTLPIIKPNR; DYFCQYCDKVYKSASKRKAHILKNHPGAELPPSIRKLRPAGPGEPDPMLSTHTQLTGTIATPPVCCPHCS; KQYSSKTKMVQHIRKKHPEFAQLSNTIHTPLTTAVISATPAVLTTDSATGETVVTTDLLTQAMTELSQTL; TTDYRTPQGDYQRIQYIPVSQSASGLQQPQHIQLQVVQVASATSPHQSQQSTVDVGQLHDPQPYPQHAIQ; VQHIQVSEPTASAPSSAQVSGQPLSPSAQQAQQGLSPSHIQGSSSTQGQALQQQQQQQQNSSVQHTYLPS; AWNSFRGYSSEIQMMTLPPGQFVITDSGVATPVTTGQVKAVTSGHYVLSESQSELEEKQTSALSGGVQVE; PPAHSDSLDPQTNSQQQTTQYIITTTTNGNGSSEVHITKP; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020228
• ORF Size: 3480 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020228.3
• RefSeq Size: 6341 bp
• RefSeq ORF: 3483 bp
• Locus ID: 56980
• UniProt ID: Q9NQV6
• Cytogenetics: 11q24.3
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Summary: The protein encoded by this gene is a transcription factor that contains C2H2-type zinc-fingers. It also contains a positive regulatory domain, which has been found in several other zinc-finger transcription factors including those involved in B cell differentiation and tumor suppression. Studies of the mouse counterpart suggest that this protein may be involved in the development of the central nerve system (CNS), as well as in the pathogenesis of neuronal storage disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]