IL18R Beta (IL18RAP) (NM_003853) Human Tagged ORF Clone

SKU RG221477

IL18RAP (tGFP-tagged) - Human interleukin 18 receptor accessory protein (IL18RAP)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: IL18R Beta
• Synonyms: ACPL; CD218b; CDw218b; IL-1R-7; IL-1R7; IL-1RAcPL; IL-18R-beta; IL-18RAcP; IL-18Rbeta; IL18RB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221477 representing NM_003853; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTCTGTTTGGGCTGGATATTTCTTTGGCTTGTTGCAGGAGAGCGAATTAAAGGATTTAATATTTCAG; GTTGTTCCACAAAAAAACTCCTTTGGACATATTCTACAAGGAGTGAAGAGGAATTTGTCTTATTTTGTGA; TTTACCAGAGCCACAGAAATCACATTTCTGCCACAGAAATCGACTCTCACCAAAACAAGTCCCTGAGCAC; CTGCCCTTCATGGGTAGTAACGACCTATCTGATGTCCAATGGTACCAACAACCTTCGAATGGAGATCCAT; TAGAGGACATTAGGAAAAGCTATCCTCACATCATTCAGGACAAATGTACCCTTCACTTTTTGACCCCAGG; GGTGAATAATTCTGGGTCATATATTTGTAGACCCAAGATGATTAAGAGCCCCTATGATGTAGCCTGTTGT; GTCAAGATGATTTTAGAAGTTAAGCCCCAGACAAATGCATCCTGTGAGTATTCCGCATCACATAAGCAAG; ACCTACTTCTTGGGAGCACTGGCTCTATTTCTTGCCCCAGTCTCAGCTGCCAAAGTGATGCACAAAGTCC; AGCGGTAACCTGGTACAAGAATGGAAAACTCCTCTCTGTGGAAAGGAGCAACCGAATCGTAGTGGATGAA; GTTTATGACTATCACCAGGGCACATATGTATGTGATTACACTCAGTCGGATACTGTGAGTTCGTGGACAG; TCAGAGCTGTTGTTCAAGTGAGAACCATTGTGGGAGACACTAAACTCAAACCAGATATTCTGGATCCTGT; CGAGGACACACTGGAAGTAGAACTTGGAAAGCCTTTAACTATTAGCTGCAAAGCACGATTTGGCTTTGAA; AGGGTCTTTAACCCTGTCATAAAATGGTACATCAAAGATTCTGACCTAGAGTGGGAAGTCTCAGTACCTG; AGGCGAAAAGTATTAAATCCACTTTAAAGGATGAAATCATTGAGCGTAATATCATCTTGGAAAAAGTCAC; TCAGCGTGATCTTCGCAGGAAGTTTGTTTGCTTTGTCCAGAACTCCATTGGAAACACAACCCAGTCCGTC; CAACTGAAAGAAAAGAGAGGAGTGGTGCTCCTGTACATCCTGCTTGGCACCATCGGGACCCTGGTGGCCG; TGCTGGCGGCGAGTGCCCTCCTCTACAGGCACTGGATTGAAATAGTGCTGCTGTACCGGACCTACCAGAG; CAAGGATCAGACGCTTGGGGATAAAAAGGATTTTGATGCTTTCGTATCCTATGCAAAATGGAGCTCTTTT; CCAAGTGAGGCCACTTCATCTCTGAGTGAAGAACACTTGGCCCTGAGCCTATTTCCTGATGTTTTAGAAA; ACAAATATGGATATAGCCTGTGTTTGCTTGAAAGAGATGTGGCTCCAGGAGGAGTGTATGCAGAAGACAT; TGTGAGCATTATTAAGAGAAGCAGAAGAGGAATATTTATCTTGAGCCCCAACTATGTCAATGGACCCAGT; ATCTTTGAACTACAAGCAGCAGTGAATCTTGCCTTGGATGATCAAACACTGAAACTCATTTTAATTAAGT; TCTGTTACTTCCAAGAGCCAGAGTCTCTACCTCATCTCGTGAAAAAAGCTCTCAGGGTTTTGCCCACAGT; TACTTGGAGAGGCTTAAAATCAGTTCCTCCCAATTCTAGGTTCTGGGCCAAAATGCGCTACCACATGCCT; GTGAAAAACTCTCAGGGATTCACGTGGAACCAGCTCAGAATTACCTCTAGGATTTTTCAGTGGAAAGGAC; TCAGTAGAACAGAAACCACTGGGAGGAGCTCCCAGCCTAAGGAATGG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221477 representing NM_003853; Red=Cloning site Green=Tags(s); ; MLCLGWIFLWLVAGERIKGFNISGCSTKKLLWTYSTRSEEEFVLFCDLPEPQKSHFCHRNRLSPKQVPEH; LPFMGSNDLSDVQWYQQPSNGDPLEDIRKSYPHIIQDKCTLHFLTPGVNNSGSYICRPKMIKSPYDVACC; VKMILEVKPQTNASCEYSASHKQDLLLGSTGSISCPSLSCQSDAQSPAVTWYKNGKLLSVERSNRIVVDE; VYDYHQGTYVCDYTQSDTVSSWTVRAVVQVRTIVGDTKLKPDILDPVEDTLEVELGKPLTISCKARFGFE; RVFNPVIKWYIKDSDLEWEVSVPEAKSIKSTLKDEIIERNIILEKVTQRDLRRKFVCFVQNSIGNTTQSV; QLKEKRGVVLLYILLGTIGTLVAVLAASALLYRHWIEIVLLYRTYQSKDQTLGDKKDFDAFVSYAKWSSF; PSEATSSLSEEHLALSLFPDVLENKYGYSLCLLERDVAPGGVYAEDIVSIIKRSRRGIFILSPNYVNGPS; IFELQAAVNLALDDQTLKLILIKFCYFQEPESLPHLVKKALRVLPTVTWRGLKSVPPNSRFWAKMRYHMP; VKNSQGFTWNQLRITSRIFQWKGLSRTETTGRSSQPKEW; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003853
• ORF Size: 1797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003853.3
• RefSeq Size: 2668 bp
• RefSeq ORF: 1800 bp
• Locus ID: 8807
• UniProt ID: O95256
• Cytogenetics: 2q12.1
• Domains: IG, TIR
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction
• Summary: The protein encoded by this gene is an accessory subunit of the heterodimeric receptor for interleukin 18 (IL18), a proinflammatory cytokine involved in inducing cell-mediated immunity. This protein enhances the IL18-binding activity of the IL18 receptor and plays a role in signaling by IL18. Mutations in this gene are associated with Crohn's disease and inflammatory bowel disease, and susceptibility to celiac disease and leprosy. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]