GPR176 (NM_007223) Human Tagged ORF Clone

SKU RG220955

GPR176 (tGFP-tagged) - Human G protein-coupled receptor 176 (GPR176)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GPR176
• Synonyms: HB-954
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220955 representing NM_007223; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGACATAACGGGAGCTGGATCTCTCCAAATGCCAGCGAGCCGCACAACGCGTCCGGCGCCGAGGCTG; CGGGTGTGAACCGCAGCGCGCTCGGGGAGTTCGGCGAGGCGCAGCTGTACCGCCAGTTCACCACCACCGT; GCAGGTCGTCATCTTCATAGGCTCGCTGCTCGGAAACTTCATGGTGTTATGGTCAACTTGCCGCACAACC; GTGTTCAAATCTGTCACCAACAGGTTCATTAAAAACCTGGCCTGCTCGGGGATTTGTGCCAGCCTGGTCT; GTGTGCCCTTCGACATCATCCTCAGCACCAGTCCTCACTGTTGCTGGTGGATCTACACCATGCTCTTCTG; CAAGGTCGTCAAATTTTTGCACAAAGTATTCTGCTCTGTGACCATCCTCAGCTTCCCTGCTATTGCTTTG; GACAGGTACTACTCAGTCCTCTATCCACTGGAGAGGAAAATATCTGATGCCAAGTCCCGTGAACTGGTGA; TGTACATCTGGGCCCATGCAGTGGTGGCCAGTGTCCCTGTGTTTGCAGTAACCAATGTGGCTGACATCTA; TGCCACGTCCACCTGCACGGAAGTCTGGAGCAACTCCTTGGGCCACCTGGTGTACGTTCTGGTGTATAAC; ATCACCACGGTCATTGTGCCTGTGGTGGTGGTGTTCCTCTTCTTGATACTGATCCGACGGGCCCTGAGTG; CCAGCCAGAAGAAGAAGGTCATCATAGCAGCGCTCCGGACCCCACAGAACACCATCTCTATTCCCTATGC; CTCCCAGCGGGAGGCCGAGCTGCACGCCACCCTGCTCTCCATGGTGATGGTCTTCATCTTGTGTAGCGTG; CCCTATGCCACCCTGGTCGTCTACCAGACTGTGCTCAATGTCCCTGACACTTCCGTCTTCTTGCTGCTCA; CTGCTGTTTGGCTGCCCAAAGTCTCCCTGCTGGCAAACCCTGTTCTCTTTCTTACTGTGAACAAATCTGT; CCGCAAGTGCTTGATAGGGACCCTGGTGCAACTACACCACCGGTACAGTCGCCGTAATGTGGTCAGTACA; GGGAGTGGCATGGCTGAGGCCAGCCTGGAACCCAGCATACGCTCGGGTAGCCAGCTCCTGGAGATGTTCC; ACATTGGGCAGCAGCAGATCTTTAAGCCCACAGAGGATGAGGAAGAGAGTGAGGCCAAGTACATTGGCTC; AGCTGACTTCCAGGCCAAGGAGATATTTAGCACCTGCCTGGAGGGAGAGCAGGGGCCACAGTTTGCGCCC; TCTGCCCCACCCCTGAGCACAGTGGACTCTGTATCCCAGGTGGCACCGGCAGCCCCTGTGGAACCTGAAA; CATTCCCTGATAAGTATTCCCTGCAGTTTGGCTTTGGGCCTTTTGAGTTGCCTCCTCAGTGGCTCTCAGA; GACCCGAAACAGCAAGAAGCGGCTGCTTCCCCCCTTGGGCAACACCCCAGAAGAGCTGATCCAGACAAAG; GTGCCCAAGGTAGGCAGGGTGGAGCGGAAGATGAGCAGAAACAATAAAGTGAGCATTTTTCCAAAGGTGG; ATTCC; ; AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220955 representing NM_007223; Red=Cloning site Green=Tags(s); ; MGHNGSWISPNASEPHNASGAEAAGVNRSALGEFGEAQLYRQFTTTVQVVIFIGSLLGNFMVLWSTCRTT; VFKSVTNRFIKNLACSGICASLVCVPFDIILSTSPHCCWWIYTMLFCKVVKFLHKVFCSVTILSFPAIAL; DRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVADIYATSTCTEVWSNSLGHLVYVLVYN; ITTVIVPVVVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVMVFILCSV; PYATLVVYQTVLNVPDTSVFLLLTAVWLPKVSLLANPVLFLTVNKSVRKCLIGTLVQLHHRYSRRNVVST; GSGMAEASLEPSIRSGSQLLEMFHIGQQQIFKPTEDEEESEAKYIGSADFQAKEIFSTCLEGEQGPQFAP; SAPPLSTVDSVSQVAPAAPVEPETFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTK; VPKVGRVERKMSRNNKVSIFPKVDS; ; SGPTRTRRLE - GFP Tag - V
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007223
• ORF Size: 1545 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007223.3
• RefSeq Size: 4542 bp
• RefSeq ORF: 1548 bp
• Locus ID: 11245
• UniProt ID: Q14439
• Cytogenetics: 15q14-q15.1
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Summary: Members of the G protein-coupled receptor family, such as GPR176, are cell surface receptors involved in responses to hormones, growth factors, and neurotransmitters (Hata et al., 1995 [PubMed 7893747]).[supplied by OMIM, Jul 2008]