HSPA4 (NM_002154) Human Tagged ORF Clone
SKU
RG220635
HSPA4 (tGFP-tagged) - Human heat shock 70kDa protein 4 (HSPA4)
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TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Type | Human Tagged ORF Clone |
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Target Symbol | HSPA4 |
Synonyms | APG-2; HEL-S-5a; HS24/P52; hsp70; hsp70RY; HSPH2; RY |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RG220635 representing NM_002154
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGGTGGGCATAGACCTGGGCTTCCAGAGCTGCTACGTCGCTGTGGCCCGCGCCGGCGGCATCG AGACTATCGCTAATGAGTATAGCGACCGCTGCACGCCGGCTTGCATTTCTTTTGGTCCTAAGAATCGTTC AATTGGAGCAGCAGCTAAAAGCCAGGTAATTTCTAATGCAAAGAACACAGTCCAAGGATTTAAAAGATTC CATGGCCGAGCATTCTCTGATCCATTTGTGGAGGCAGAAAAATCTAACCTTGCATATGATATTGTGCAGT TGCCTACAGGATTAACAGGTATAAAGGTGACATATATGGAGGAAGAGCGAAATTTTACCACTGAGCAAGT GACTGCCATGCTTTTGTCCAAACTGAAGGAGACAGCCGAAAGTGTTCTTAAGAAGCCTGTAGTTGACTGT GTTGTTTCGGTTCCTTGTTTCTATACTGATGCAGAAAGACGATCAGTGATGGATACAACACAGATTGCTG GTCTTAATTGCTTGCGATTAATGAATGAAACCACTGCAGTTGCTCTTGCATATGGAATCTATAAGCAGGA TCTTCCTGCCTTAGAAGAGAAACCAAGAAATGTAGTTTTTGTAGACATGGGCCACTCTGCTTATCAAGTT TCTGTATGTGCATTTAATAGAGGAAAACTGAAAGTTCTGGCCACTGCATTTGACACGACATTGGGAGGTA GAAAATTTGATGAAGTGTTAGTAAATCACTTCTGTGAAGAATTTGGGAAGAAATACAAGCTAGACATTAA GTCCAAAATCCGTGCATTATTACGACTCTCTCAGGAGTGTGAGAAACTCAAGAAATTGATGAGTGCAAAT GCTTCAGATCTCCCTTTGAGCATTGAATGTTTTATGAATGATGTTGATGTATCTGGAACTATGAATAGAG GCAAATTTCTGGAGATGTGCAATGATCTCTTAGCTAGAGTGGAGCCACCACTTCGTAGTGTTTTGGAACA AACCAAGTTAAAGAAAGAAGATATTTATGCAGTGGAGATAGTTGGTGGTGCTACACGAATCCCTGCGGTA AAAGAGAAGATCAGCAAATTTTTCGGTAAAGAACTTAGTACAACATTAAATGCTGATGAAGCTGTCACTC GAGGCTGTGCATTGCAGTGTGCCATCTTATCGCCTGCTTTCAAAGTCAGAGAATTTTCTATCACTGATGT AGTACCATATCCAATATCTCTGAGATGGAATTCTCCAGCTGAAGAAGGGTCAAGTGACTGTGAAGTCTTT TCCAAAAATCATGCTGCTCCTTTCTCTAAAGTTCTTACATTTTATAGAAAGGAACCTTTCACTCTTGAGG CCTACTACAGCTCTCCTCAGGATTTGCCCTATCCAGATCCTGCTATAGCTCAGTTTTCAGTTCAGAAAGT CACTCCTCAGTCTGATGGCTCCAGTTCAAAAGTGAAAGTCAAAGTTCGAGTAAATGTCCATGGCATTTTC AGTGTGTCCAGTGCATCTTTAGTGGAGGTTCACAAGTCTGAGGAAAATGAGGAGCCAATGGAAACAGATC AGAATGCAAAGGAGGAAGAGAAGATGCAAGTGGACCAGGAGGAACCACATGTTGAAGAGCAACAGCAGCA GACACCAGCAGAAAATAAGGCAGAGTCTGAAGAAATGGAGACCTCTCAAGCTGGATCCAAGGATAAAAAG ATGGACCAACCACCCCAAGCCAAGAAGGCAAAAGTGAAGACCAGTACTGTGGACCTGCCAATCGAGAATC AGCTATTATGGCAGATAGACAGAGAGATGCTCAACTTGTACATTGAAAATGAGGGTAAGATGATCATGCA GGATAAACTGGAGAAGGAGCGGAATGATGCTAAGAACGCAGTGGAGGAATATGTGTATGAAATGAGAGAC AAGCTTAGTGGTGAATATGAGAAGTTTGTGAGTGAAGATGATCGTAACAGTTTTACTTTGAAACTGGAAG ATACTGAAAATTGGTTGTATGAGGATGGAGAAGACCAGCCAAAGCAAGTTTATGTTGATAAGTTGGCTGA ATTAAAAAATCTAGGTCAACCTATTAAGATACGTTTCCAGGAATCTGAAGAACGACCAAAATTATTTGAA GAACTAGGGAAACAGATCCAACAGTATATGAAAATAATCAGCTCTTTCAAAAACAAGGAGGACCAGTATG ATCATTTGGATGCTGCTGACATGACAAAGGTAGAAAAAAGCACAAATGAAGCAATGGAGTGGATGAATAA CAAGCTAAATCTGCAGAACAAGCAGAGTTTGACCATGGATCCAGTTGTCAAGTCAAAAGAGATTGAAGCT AAAATTAAGGAGCTGACAAGTACTTGTAGCCCTATAATTTCAAAGCCCAAACCCAAAGTGGAACCTCCAA AAGAGGAACAAAAAAATGCAGAGCAGAATGGACCAGTGGATGGACAAGGAGACAACCCAGGCCCCCAGGC TGCTGAGCAGGGTACAGACACAGCTGTGCCTTCGGATTCAGACAAGAAGCTTCCTGAAATGGACATTGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG220635 representing NM_002154
Red=Cloning site Green=Tags(s) MSVVGIDLGFQSCYVAVARAGGIETIANEYSDRCTPACISFGPKNRSIGAAAKSQVISNAKNTVQGFKRF HGRAFSDPFVEAEKSNLAYDIVQLPTGLTGIKVTYMEEERNFTTEQVTAMLLSKLKETAESVLKKPVVDC VVSVPCFYTDAERRSVMDTTQIAGLNCLRLMNETTAVALAYGIYKQDLPALEEKPRNVVFVDMGHSAYQV SVCAFNRGKLKVLATAFDTTLGGRKFDEVLVNHFCEEFGKKYKLDIKSKIRALLRLSQECEKLKKLMSAN ASDLPLSIECFMNDVDVSGTMNRGKFLEMCNDLLARVEPPLRSVLEQTKLKKEDIYAVEIVGGATRIPAV KEKISKFFGKELSTTLNADEAVTRGCALQCAILSPAFKVREFSITDVVPYPISLRWNSPAEEGSSDCEVF SKNHAAPFSKVLTFYRKEPFTLEAYYSSPQDLPYPDPAIAQFSVQKVTPQSDGSSSKVKVKVRVNVHGIF SVSSASLVEVHKSEENEEPMETDQNAKEEEKMQVDQEEPHVEEQQQQTPAENKAESEEMETSQAGSKDKK MDQPPQAKKAKVKTSTVDLPIENQLLWQIDREMLNLYIENEGKMIMQDKLEKERNDAKNAVEEYVYEMRD KLSGEYEKFVSEDDRNSFTLKLEDTENWLYEDGEDQPKQVYVDKLAELKNLGQPIKIRFQESEERPKLFE ELGKQIQQYMKIISSFKNKEDQYDHLDAADMTKVEKSTNEAMEWMNNKLNLQNKQSLTMDPVVKSKEIEA KIKELTSTCSPIISKPKPKVEPPKEEQKNAEQNGPVDGQGDNPGPQAAEQGTDTAVPSDSDKKLPEMDID TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002154 |
ORF Size | 2520 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_002154.3, NP_002145.3 |
RefSeq Size | 3385 bp |
RefSeq ORF | 2523 bp |
Locus ID | 3308 |
UniProt ID | P34932 |
Cytogenetics | 5q31.1 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS |
Protein Pathways | Antigen processing and presentation |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
RC220635 | HSPA4 (Myc-DDK-tagged)-Human heat shock 70kDa protein 4 (HSPA4) | 10 ug |
$1,153.00
|
|
RC220635L1 | Lenti ORF clone of Human heat shock 70kDa protein 4 (HSPA4), Myc-DDK-tagged | 10 ug |
$1,453.00
|
|
RC220635L2 | Lenti ORF clone of Human heat shock 70kDa protein 4 (HSPA4), mGFP tagged | 10 ug |
$1,453.00
|
|
RC220635L3 | Lenti ORF clone of Human heat shock 70kDa protein 4 (HSPA4), Myc-DDK-tagged | 10 ug |
$1,453.00
|
|
RC220635L4 | Lenti ORF clone of Human heat shock 70kDa protein 4 (HSPA4), mGFP tagged | 10 ug |
$1,453.00
|
|
SC107571 | HSPA4 (untagged)-Human heat shock 70kDa protein 4 (HSPA4) | 10 ug |
$1,155.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.