KLC1 (NM_182923) Human Tagged ORF Clone

SKU RG220210

KLC1 (tGFP-tagged) - Human kinesin light chain 1 (KLC1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: KLC1
• Synonyms: KLC; KNS2; KNS2A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220210 representing NM_182923; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTATGACACCATGTCCACAATGGTGTACATAAAGGAAGACAAGTTGGAGAAGCTTACACAGGATGAAA; TTATTTCTAAGACAAAGCAAGTAATTCAGGGGCTGGAAGCTTTGAAGAATGAGCACAATTCCATTTTACA; AAGTTTGCTGGAGACACTGAAGTGTTTGAAGAAAGATGATGAAAGTAATTTGGTGGAGGAGAAATCAAAC; ATGATCCGGAAGTCACTGGAGATGTTGGAGCTCGGCCTGAGTGAGGCACAGGTTATGATGGCTTTGTCAA; ATCACCTGAATGCTGTGGAGTCCGAGAAGCAGAAACTGCGTGCGCAGGTTCGTCGTCTGTGCCAGGAGAA; TCAGTGGCTACGGGATGAACTGGCCAACACGCAGCAGAAACTGCAGAAGAGTGAGCAGTCTGTGGCTCAA; CTGGAGGAGGAGAAGAAGCATCTGGAGTTTATGAATCAGCTAAAAAAATATGATGACGACATTTCCCCAT; CCGAGGACAAAGACACTGATTCTACCAAAGAGCCTCTGGATGACCTTTTCCCCAATGATGAAGACGACCC; AGGGCAAGGAATCCAGCAGCAGCACAGCAGTGCAGCCGCGGCTGCCCAGCAGGGCGGCTACGAGATCCCC; GCGCGGCTGCGGACGCTCCACAACCTGGTGATCCAGTACGCCTCGCAGGGGCGCTACGAGGTAGCTGTGC; CCCTCTGCAAGCAGGCCCTGGAGGACCTGGAGAAGACTTCAGGACACGACCACCCGGACGTGGCCACCAT; GCTCAACATCCTGGCCTTGGTGTACAGGGATCAGAATAAATACAAAGATGCAGCTAACCTACTGAATGAT; GCCTTGGCTATTCGTGAGAAAACTTTGGGCAAAGATCATCCTGCGGTGGCGGCGACTTTGAATAACCTTG; CAGTCCTTTATGGTAAAAGAGGGAAGTACAAAGAAGCAGAGCCGTTGTGTAAAAGAGCTCTGGAAATCCG; AGAAAAGGTTTTGGGGAAGGATCACCCCGATGTTGCCAAGCAGTTAAATAACTTGGCCTTACTGTGCCAG; AACCAGGGCAAGTATGAAGAAGTAGAATATTATTATCAAAGAGCCCTCGAGATCTACCAGACAAAACTGG; GACCTGATGACCCCAACGTGGCTAAGACGAAAAATAACCTGGCATCCTGCTATTTGAAACAAGGAAAGTT; CAAGCAAGCAGAAACACTGTACAAAGAGATTCTCACTCGTGCACATGAAAGGGAGTTTGGTTCTGTAGAT; GATGAAAATAAACCCATCTGGATGCATGCTGAAGAAAGAGAAGAATGCAAAGGAAAGCAAAAGGATGGGA; CATCTTTTGGAGAGTATGGCGGCTGGTACAAAGCCTGCAAAGTTGATAGTCCAACTGTTACAACCACTCT; AAAAAACCTTGGGGCACTTTACAGACGTCAAGGCAAATTTGAAGCTGCAGAAACGTTAGAAGAAGCTGCT; ATGAGGTCTCGTAAACAGGGTCTTGACAATGTTCACAAACAGAGGGTGGCAGAAGTGCTCAATGACCCTG; AGAACATGGAGAAGCGCAGGAGCCGTGAGAGCCTCAACGTGGACGTGGTCAAGTACGAGAGTGGCCCTGA; CGGAGGGGAGGAAGTGAGTATGAGCGTAGAGTGGAACGGGGACGGCACTGGATCTTTAAAACGCAGTGGT; TCCTTTAGCAAACTCCGGGCTTCCATTAGACGCAGCAGTGAGAAGCTGGTTAGGAAGCTGAAGGGAGGAA; GTTCACGAGAGAGTGAGCCAAAGAACCCCGGGGTCTCTGGCCGAGCCTCTTTTTGTGGAAAACGACAGCA; GCAGCAGTGGCCTGGAAGACGCCACCGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220210 representing NM_182923; Red=Cloning site Green=Tags(s); ; MYDTMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSN; MIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQ; LEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLFPNDEDDPGQGIQQQHSSAAAAAQQGGYEIP; ARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLND; ALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQ; NQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVD; DENKPIWMHAEEREECKGKQKDGTSFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAA; MRSRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGEEVSMSVEWNGDGTGSLKRSG; SFSKLRASIRRSSEKLVRKLKGGSSRESEPKNPGVSGRASFCGKRQQQQWPGRRHR; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182923
• ORF Size: 1848 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_182923.2, NP_891553.1
• RefSeq Size: 2548 bp
• RefSeq ORF: 1722 bp
• Locus ID: 3831
• UniProt ID: Q07866
• Cytogenetics: 14q32.33
• Protein Families: Druggable Genome
• Summary: Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined. [provided by RefSeq, Jul 2008]