SALL3 (NM_171999) Human Tagged ORF Clone

SKU RG219967

SALL3 (tGFP-tagged) - Human sal-like 3 (Drosophila) (SALL3)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SALL3
• Synonyms: ZNF796
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219967 representing NM_171999; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCTCGGCGCAAGCAGGCCAAGCCCCAGCACCTCAAGTCGGACGAGGAGCTGCTGCCGCCTGACGGGG; CTCCCGAGCACGCCGCCCCGGGGGAAGGTGCGGAGGACGCAGACAGCGGGCCCGAGAGCCGCAGCGGGGG; CGAGGAGACCAGCGTGTGCGAGAAATGCTGCGCCGAGTTCTTCAAGTGGGCGGACTTCCTGGAGCACCAG; CGGAGCTGCACCAAGCTCCCGCCCGTGCTGATCGTGCACGAGGACGCGCCCGCGCCGCCCCACGAGGACT; TCCCCGAGCCTTCGCCCGCCAGCTCCCCCAGCGAGCGCGCCGAAAGCGAGGCGGCCGAGGAGGCGGGTGC; GGAGGGCGCGGAGGGCGAGGCCAGGCCGGTGGAGAAGGAGGCCGAGCCCATGGACGCGGAACCCGCGGGG; GACACGCGCGCGCCCCGGCCCCCGCCTGCGGCCCCTGCACCCCCAACGCCCGCCTACGGCGCGCCCAGCA; CCAACGTGACCCTGGAGGCGCTGCTGAGCACCAAGGTGGCGGTGGCGCAGTTCTCGCAGGGCGCGCGCGC; GGCAGGCGGCTCGGGAGCAGGTGGAGGCGTGGCAGCTGCAGCCGTGCCCCTGATCCTGGAACAGCTCATG; GCCCTGCAGCAGCAGCAGATCCACCAGCTGCAGCTCATCGAGCAGATCCGCAGCCAGGTGGCCCTCATGC; AGCGCCCGCCGCCGCGGCCCTCACTCAGCCCCGCGGCCGCCCCGAGCGCACCGGGCCCGGCCCCCAGCCA; GCTGCCCGGGCTGGCCGCGCTCCCGCTGTCGGCCGGGGCCCCTGCCGCCGCCATCGCGGGCTCGGGCCCC; GCCGCCCCGGCCGCCTTCGAGGGCGCGCAGCCGCTGTCCCGGCCCGAGTCTGGCGCCAGCACCCCCGGCG; GCCCTGCGGAGCCCAGCGCGCCCGCCGCCCCCAGCGCCGCCCCTGCCCCCGCTGCCCCCGCCCCGGCGCC; AGCGCCGCAGAGCGCAGCCTCGTCGCAGCCGCAGAGCGCATCCACGCCGCCTGCCCTGGCCCCGGGGTCC; CTGCTGGGTGCGGCGCCCGGCCTGCCAAGTCCGCTTCTACCTCAGACTTCCGCCAGCGGCGTCATCTTCC; CCAACCCGCTGGTCAGCATCGCGGCCACGGCCAACGCTCTGGACCCGCTGTCCGCGCTCATGAAGCACCG; CAAGGGCAAGCCGCCCAATGTGTCGGTGTTCGAGCCCAAAGCCAGCGCCGAGGACCCGTTCTTCAAGCAC; AAATGCCGCTTCTGCGCCAAGGTCTTCGGCAGCGACAGCGCGCTCCAGATCCACCTGCGCTCGCACACAG; GCGAGCGGCCCTTCAAGTGCAACATCTGCGGGAACCGCTTCTCCACCAAAGGCAACCTGAAGGTGCACTT; CCAGAGGCACAAGGAGAAGTACCCCCACATCCAGATGAACCCTTACCCGGTCCCCGAGTACCTGGACAAC; GTGCCCACCTGCTCGGGCATCCCCTACGGCATGTCGCTGCCCCCCGAGAAGCCCGTGACCACCTGGCTGG; ACAGCAAGCCCGTGCTGCCCACCGTGCCCACGTCCGTGGGGCTGCAACTGCCGCCCACTGTCCCTGGCGC; GCACGGCTACGCCGACTCTCCCAGCGCCACCCCAGCCAGCCGCTCCCCGCAGAGGCCCTCGCCCGCCTCC; AGCGAGTGCGCCTCCTTGTCCCCAGGCCTCAACCACGTGGAGTCCGGCGTGTCGGCCACCGCCGAGTCCC; CACAGTCGCTCCTCGGCGGGCCGCCCGTCACTAAAGCCGAGCCCGTCAGCCTGCCCTGCACCAACGCCAG; GGCCGGGGACGCTCCCGTGGGCGCGCAGGCTAGCGCTGCACCCACATCGGTGGACGGCGCACCCACGAGC; CTCGGCAGCCCCGGGCTGCCCGCCGTCTCCGAGCAGTTCAAGGCCCAGTTTCCGTTCGGGGGGCTGCTAG; ACTCGATGCAAACGTCGGAAACCTCGAAGCTGCAGCAGCTGGTGGAGAACATCGACAAGAAGATGACGGA; CCCGAACCAGTGCGTCATCTGCCACCGGGTGCTGAGCTGCCAGAGCGCGCTGAAGATGCACTACCGGACG; CACACGGGGGAGCGGCCGTTCAAGTGCAAGATCTGCGGCCGCGCCTTCACCACCAAGGGCAACCTCAAGA; CGCACTTCGGCGTGCACCGTGCAAAGCCGCCCCTGCGCGTGCAGCACTCCTGCCCCATCTGCCAGAAGAA; GTTCACCAACGCCGTGGTCCTGCAGCAGCACATCCGCATGCACATGGGCGGCCAGATCCCCAACACGCCG; CTGCCGGAGGGCTTCCAGGATGCCATGGACTCCGAGCTGGCCTACGACGACAAGAACGCGGAGACCCTGA; GCAGCTACGATGACGACATGGACGAGAACTCCATGGAGGACGACGCTGAGCTGAAGGACGCGGCCACCGA; CCCGGCCAAGCCACTCCTGTCCTATGCGGGGTCCTGCCCGCCCTCCCCGCCCTCGGTCATCTCCAGCATT; GCCGCCCTGGAGAACCAGATGAAGATGATCGACTCGGTCATGAGCTGCCAGCAGCTGACCGGCCTCAAGT; CCGTGGAGAACGGGTCCGGGGAGAGTGACCGCCTGAGCAACGACTCCTCGTCGGCCGTGGGCGACCTGGA; GAGCCGCAGCGCGGGCAGCCCCGCCCTGTCCGAGTCCTCGTCCTCGCAGGCCCTGTCGCCGGCCCCCAGC; AATGGTGAGAGCTTCCGCTCCAAGTCCCCGGGCCTGGGCGCCCCGGAGGAGCCCCAGGAAATCCCGCTCA; AGACCGAGAGGCCGGACAGCCCAGCCGCCGCCCCGGGCAGCGGAGGCGCCCCTGGCCGCGCGGGCATCAA; GGAGGAGGCGCCCTTCAGCCTGCTGTTCCTGAGCAGGGAGCGGGGTAAGTGTCCCAGCACTGTGTGTGGT; GTCTGTGGCAAGCCTTTTGCTTGCAAGAGCGCGTTGGAAATCCACTACCGCAGCCATACTAAGGAGCGGC; CATTCGTCTGCGCGCTCTGCAGGCGAGGGTGCTCCACTATGGGTAATTTAAAACAGCACTTACTGACACA; CAGATTGAAAGAGCTGCCTTCTCAGTTATTTGACCCCAACTTTGCTCTAGGTCCCAGCCAAAGCACTCCT; AGCCTGATCTCCAGCGCCGCACCCACCATGATCAAAATGGAAGTGAACGGTCACGGCAAGGCCATGGCGC; TGGGCGAGGGTCCCCCGCTGCCCGCGGGCGTCCAGGTCCCCGCCGGGCCTCAGACAGTGATGGGCCCGGG; CCTGGCGCCCATGCTGGCCCCCCCACCGCGCCGGACGCCCAAGCAGCACAACTGCCAGTCGTGCGGGAAG; ACCTTCTCCTCGGCCAGCGCCCTGCAGATCCATGAGCGCACGCACACCGGCGAGAAGCCGTTCGGCTGCA; CCATCTGCGGCCGGGCCTTCACCACTAAGGGCAACCTCAAGGTGCACATGGGGACACACATGTGGAATAA; CGCCCCCGCGAGACGCGGCCGCCGCCTGTCTGTGGAGAACCCCATGGCTCTCCTAGGGGGTGATGCCCTG; AAGTTCTCTGAAATGTTCCAGAAGGACCTGGCAGCTCGGGCAATGAACGTCGACCCCAGTTTTTGGAACC; AGTATGCTGCAGCCATCACTAACGGGCTCGCCATGAAGAACAACGAGATCTCCGTCATCCAGAACGGCGG; CATCCCCCAGCTCCCCGTGAGTCTTGGGGGCAGCGCCCTCCCCCCTCTGGGCAGCATGGCCAGTGGGATG; GACAAAGCACGCACTGGCAGTAGCCCACCCATCGTCAGCTTGGACAAAGCGAGCTCAGAAACAGCAGCCA; GCCGCCCATTCACGCGGTTTATCGAGGATAACAAGGAGATTGGTATCAAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219967 representing NM_171999; Red=Cloning site Green=Tags(s); ; MSRRKQAKPQHLKSDEELLPPDGAPEHAAPGEGAEDADSGPESRSGGEETSVCEKCCAEFFKWADFLEHQ; RSCTKLPPVLIVHEDAPAPPHEDFPEPSPASSPSERAESEAAEEAGAEGAEGEARPVEKEAEPMDAEPAG; DTRAPRPPPAAPAPPTPAYGAPSTNVTLEALLSTKVAVAQFSQGARAAGGSGAGGGVAAAAVPLILEQLM; ALQQQQIHQLQLIEQIRSQVALMQRPPPRPSLSPAAAPSAPGPAPSQLPGLAALPLSAGAPAAAIAGSGP; AAPAAFEGAQPLSRPESGASTPGGPAEPSAPAAPSAAPAPAAPAPAPAPQSAASSQPQSASTPPALAPGS; LLGAAPGLPSPLLPQTSASGVIFPNPLVSIAATANALDPLSALMKHRKGKPPNVSVFEPKASAEDPFFKH; KCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEYLDN; VPTCSGIPYGMSLPPEKPVTTWLDSKPVLPTVPTSVGLQLPPTVPGAHGYADSPSATPASRSPQRPSPAS; SECASLSPGLNHVESGVSATAESPQSLLGGPPVTKAEPVSLPCTNARAGDAPVGAQASAAPTSVDGAPTS; LGSPGLPAVSEQFKAQFPFGGLLDSMQTSETSKLQQLVENIDKKMTDPNQCVICHRVLSCQSALKMHYRT; HTGERPFKCKICGRAFTTKGNLKTHFGVHRAKPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTP; LPEGFQDAMDSELAYDDKNAETLSSYDDDMDENSMEDDAELKDAATDPAKPLLSYAGSCPPSPPSVISSI; AALENQMKMIDSVMSCQQLTGLKSVENGSGESDRLSNDSSSAVGDLESRSAGSPALSESSSSQALSPAPS; NGESFRSKSPGLGAPEEPQEIPLKTERPDSPAAAPGSGGAPGRAGIKEEAPFSLLFLSRERGKCPSTVCG; VCGKPFACKSALEIHYRSHTKERPFVCALCRRGCSTMGNLKQHLLTHRLKELPSQLFDPNFALGPSQSTP; SLISSAAPTMIKMEVNGHGKAMALGEGPPLPAGVQVPAGPQTVMGPGLAPMLAPPPRRTPKQHNCQSCGK; TFSSASALQIHERTHTGEKPFGCTICGRAFTTKGNLKVHMGTHMWNNAPARRGRRLSVENPMALLGGDAL; KFSEMFQKDLAARAMNVDPSFWNQYAAAITNGLAMKNNEISVIQNGGIPQLPVSLGGSALPPLGSMASGM; DKARTGSSPPIVSLDKASSETAASRPFTRFIEDNKEIGIN; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_171999
• ORF Size: 3900 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_171999.1, NP_741996.1
• RefSeq Size: 4772 bp
• RefSeq ORF: 3903 bp
• Locus ID: 27164
• UniProt ID: Q9BXA9
• Cytogenetics: 18q23
• Summary: This gene encodes a sal-like C2H2-type zinc-finger protein, and belongs to a family of evolutionarily conserved genes found in species as diverse as Drosophila, C. elegans, and vertebrates. Mutations in some of these genes are associated with congenital disorders in human, suggesting their importance in embryonic development. This protein binds to DNA methyltransferase 3 alpha (DNMT3A), and reduces DNMT3A-mediated CpG island methylation. It is suggested that silencing of this gene, resulting in acceleration of DNA methylation, may have a role in oncogenesis. [provided by RefSeq, Oct 2011]