Band 3 (SLC4A1) (NM_000342) Human Tagged ORF Clone

SKU RG218312

SLC4A1 (tGFP-tagged) - Human solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group) (SLC4A1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Band 3
• Synonyms: AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR; RTA1A; SAO; SPH4; SW; WD; WD1; WR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218312 representing NM_000342; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGGAGCTGCAGGATGATTATGAAGACATGATGGAGGAGAATCTGGAGCAGGAGGAATATGAAGACC; CAGACATCCCCGAGTCCCAGATGGAGGAGCCGGCAGCTCACGACACCGAGGCAACAGCCACAGACTACCA; CACCACATCACACCCGGGTACCCACAAGGTCTATGTGGAGCTGCAGGAGCTGGTGATGGACGAAAAGAAC; CAGGAGCTGAGATGGATGGAGGCGGCGCGCTGGGTGCAACTGGAGGAGAACCTGGGGGAGAATGGGGCCT; GGGGCCGCCCGCACCTCTCTCACCTCACCTTCTGGAGCCTCCTAGAGCTGCGTAGAGTCTTCACCAAGGG; TACTGTTCTCCTAGACCTGCAAGAGACCTCCCTGGCTGGAGTGGCCAACCAACTGCTAGACAGGTTTATC; TTTGAAGACCAGATCCGGCCTCAGGACCGAGAGGAGCTGCTCCGGGCCCTGCTGCTTAAACACAGCCACG; CTGGAGAGCTGGAGGCCCTGGGGGGTGTGAAGCCTGCAGTCCTGACACGCTCTGGGGATCCTTCACAGCC; TCTGCTCCCCCAACACTCCTCACTGGAGACACAGCTCTTCTGTGAGCAGGGAGATGGGGGCACAGAAGGG; CACTCACCATCTGGAATTCTGGAAAAGATTCCCCCGGATTCAGAGGCCACGTTGGTGCTAGTGGGCCGCG; CCGACTTCCTGGAGCAGCCGGTGCTGGGCTTCGTGAGGCTGCAGGAGGCAGCGGAGCTGGAGGCGGTGGA; GCTGCCGGTGCCTATACGCTTCCTCTTTGTGTTGCTGGGACCTGAGGCCCCCCACATCGATTACACCCAG; CTTGGCCGGGCTGCTGCCACCCTCATGTCAGAGAGGGTGTTCCGCATAGATGCCTACATGGCTCAGAGCC; GAGGGGAGCTGCTGCACTCCCTAGAGGGCTTCCTGGACTGCAGCCTAGTGCTGCCTCCCACCGATGCCCC; CTCCGAGCAGGCACTGCTCAGTCTGGTGCCTGTGCAGAGGGAGCTACTTCGAAGGCGCTATCAGTCCAGC; CCTGCCAAGCCAGACTCCAGCTTCTACAAGGGCCTAGACTTAAATGGGGGCCCAGATGACCCTCTGCAGC; AGACAGGCCAGCTCTTCGGGGGCCTGGTGCGTGATATCCGGCGCCGCTACCCCTATTACCTGAGTGACAT; CACAGATGCATTCAGCCCCCAGGTCCTGGCTGCCGTCATCTTCATCTACTTTGCTGCACTGTCACCCGCC; ATCACCTTCGGCGGCCTCCTGGGAGAAAAGACCCGGAACCAGATGGGAGTGTCGGAGCTGCTGATCTCCA; CTGCAGTGCAGGGCATTCTCTTCGCCCTGCTGGGGGCTCAGCCCCTGCTTGTGGTCGGCTTCTCAGGACC; CCTGCTGGTGTTTGAGGAAGCCTTCTTCTCGTTCTGCGAGACCAACGGTCTAGAGTACATCGTGGGCCGC; GTGTGGATCGGCTTCTGGCTCATCCTGCTGGTGGTGTTGGTGGTGGCCTTCGAGGGTAGCTTCCTGGTCC; GCTTCATCTCCCGCTATACCCAGGAGATCTTCTCCTTCCTCATTTCCCTCATCTTCATCTATGAGACTTT; CTCCAAGCTGATCAAGATCTTCCAGGACCACCCACTACAGAAGACTTATAACTACAACGTGTTGATGGTG; CCCAAACCTCAGGGCCCCCTGCCCAACACAGCCCTCCTCTCCCTTGTGCTCATGGCCGGTACCTTCTTCT; TTGCCATGATGCTGCGCAAGTTCAAGAACAGCTCCTATTTCCCTGGCAAGCTGCGTCGGGTCATCGGGGA; CTTCGGGGTCCCCATCTCCATCCTGATCATGGTCCTGGTGGATTTCTTCATTCAGGATACCTACACCCAG; AAACTCTCGGTGCCTGATGGCTTCAAGGTGTCCAACTCCTCAGCCCGGGGCTGGGTCATCCACCCACTGG; GCTTGCGTTCCGAGTTTCCCATCTGGATGATGTTTGCCTCCGCCCTGCCTGCTCTGCTGGTCTTCATCCT; CATATTCCTGGAGTCTCAGATCACCACGCTGATTGTCAGCAAACCTGAGCGCAAGATGGTCAAGGGCTCC; GGCTTCCACCTGGACCTGCTGCTGGTAGTAGGCATGGGTGGGGTGGCCGCCCTCTTTGGGATGCCCTGGC; TCAGTGCCACCACCGTGCGTTCCGTCACCCATGCCAACGCCCTCACTGTCATGGGCAAAGCCAGCACCCC; AGGGGCTGCAGCCCAGATCCAGGAGGTCAAAGAGCAGCGGATCAGTGGACTCCTGGTCGCTGTGCTTGTG; GGCCTGTCCATCCTCATGGAGCCCATCCTGTCCCGCATCCCCCTGGCTGTACTGTTTGGCATCTTCCTCT; ACATGGGGGTCACGTCGCTCAGCGGCATCCAGCTCTTTGACCGCATCTTGCTTCTGTTCAAGCCACCCAA; GTATCACCCAGATGTGCCCTACGTCAAGCGGGTGAAGACCTGGCGCATGCACTTATTCACGGGCATCCAG; ATCATCTGCCTGGCAGTGCTGTGGGTGGTGAAGTCCACGCCGGCCTCCCTGGCCCTGCCCTTCGTCCTCA; TCCTCACTGTGCCGCTGCGGCGCGTCCTGCTGCCGCTCATCTTCAGGAACGTGGAGCTTCAGTGTCTGGA; TGCTGATGATGCCAAGGCAACCTTTGATGAGGAGGAAGGTCGGGATGAATACGACGAAGTGGCCATGCCT; GTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218312 representing NM_000342; Red=Cloning site Green=Tags(s); ; MEELQDDYEDMMEENLEQEEYEDPDIPESQMEEPAAHDTEATATDYHTTSHPGTHKVYVELQELVMDEKN; QELRWMEAARWVQLEENLGENGAWGRPHLSHLTFWSLLELRRVFTKGTVLLDLQETSLAGVANQLLDRFI; FEDQIRPQDREELLRALLLKHSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLFCEQGDGGTEG; HSPSGILEKIPPDSEATLVLVGRADFLEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQ; LGRAAATLMSERVFRIDAYMAQSRGELLHSLEGFLDCSLVLPPTDAPSEQALLSLVPVQRELLRRRYQSS; PAKPDSSFYKGLDLNGGPDDPLQQTGQLFGGLVRDIRRRYPYYLSDITDAFSPQVLAAVIFIYFAALSPA; ITFGGLLGEKTRNQMGVSELLISTAVQGILFALLGAQPLLVVGFSGPLLVFEEAFFSFCETNGLEYIVGR; VWIGFWLILLVVLVVAFEGSFLVRFISRYTQEIFSFLISLIFIYETFSKLIKIFQDHPLQKTYNYNVLMV; PKPQGPLPNTALLSLVLMAGTFFFAMMLRKFKNSSYFPGKLRRVIGDFGVPISILIMVLVDFFIQDTYTQ; KLSVPDGFKVSNSSARGWVIHPLGLRSEFPIWMMFASALPALLVFILIFLESQITTLIVSKPERKMVKGS; GFHLDLLLVVGMGGVAALFGMPWLSATTVRSVTHANALTVMGKASTPGAAAQIQEVKEQRISGLLVAVLV; GLSILMEPILSRIPLAVLFGIFLYMGVTSLSGIQLFDRILLLFKPPKYHPDVPYVKRVKTWRMHLFTGIQ; IICLAVLWVVKSTPASLALPFVLILTVPLRRVLLPLIFRNVELQCLDADDAKATFDEEEGRDEYDEVAMP; V; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000342
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000342.2
• RefSeq Size: 3637 bp
• RefSeq ORF: 2736 bp
• Locus ID: 6521
• UniProt ID: P02730
• Cytogenetics: 17q21.31
• Domains: HCO3_cotransp
• Protein Families: Druggable Genome, Transmembrane
• Summary: The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis. [provided by RefSeq, Jul 2008]