LPHN1 (ADGRL1) (NM_014921) Human Tagged ORF Clone

SKU RG217296

LPHN1 (tGFP-tagged) - Human latrophilin 1 (LPHN1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: LPHN1
• Synonyms: CIRL1; CL1; LEC2; LPHN1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217296 representing NM_014921; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCCGCCTAGCCGCAGTGCTCTGGAATCTGTGTGTCACCGCCGTCCTGGTCACCTCGGCCACCCAAG; GCCTGAGCCGGGCCGGGCTCCCGTTCGGGCTGATGCGCCGGGAGCTGGCGTGTGAAGGCTACCCCATCGA; GCTGCGGTGCCCCGGCAGCGACGTCATCATGGTGGAGAATGCCAACTACGGGCGCACGGACGACAAGATT; TGCGATGCTGACCCTTTCCAGATGGAGAATGTGCAGTGCTACCTGCCGGACGCCTTCAAGATCATGTCAC; AGAGGTGTAACAACCGCACCCAGTGCGTGGTGGTCGCCGGCTCGGATGCCTTTCCTGACCCCTGTCCTGG; GACCTACAAGCACCTGGAGGTGCAGTACGACTGTGTCCCCTACATCTTCGTGTGCCCAGGGACCCTGCAG; AAGGTGCTGGAGCCCACCTCGACACACGAGTCAGAGCACCAGTCTGGCGCATGGTGCAAGGACCCGCTGC; AGGCGGGTGACCGCATCTACGTGATGCCCTGGATCCCCTACCGCACGGACACACTGACTGAGTATGCCTC; GTGGGAGGACTACGTGGCCGCCCGCCACACCACCACCTACCGCCTGCCCAACCGCGTGGATGGCACAGGC; TTTGTGGTCTACGATGGTGCCGTCTTCTACAACAAGGAGCGCACGCGCAACATCGTCAAGTATGACCTAC; GGACGCGCATCAAGAGCGGGGAGACGGTCATCAATACCGCCAACTACCATGACACCTCGCCCTACCGCTG; GGGCGGAAAGACCGACATTGACCTGGCGGTGGACGAGAACGGGCTGTGGGTCATCTACGCCACTGAGGGC; AACAACGGGCGGCTGGTGGTGAGCCAGCTGAACCCCTACACACTGCGCTTTGAGGGCACGTGGGAGACGG; GTTACGACAAGCGCTCGGCATCCAACGCCTTCATGGTGTGTGGGGTCCTGTACGTCCTGCGTTCCGTGTA; CGTGGATGATGACAGCGAGGCGGCTGGCAACCGCGTGGACTATGCCTTCAACACCAATGCCAACCGCGAG; GAGCCTGTCAGCCTCACCTTCCCCAACCCCTACCAGTTCATCTCCTCCGTTGACTACAACCCTCGCGACA; ACCAGCTGTACGTCTGGAACAACTATTTCGTGGTGCGCTACAGCCTGGAGTTCGGGCCGCCCGACCCCAG; TGCTGGCCCAGCCACTTCCCCACCCCTCAGCACGACCACCACAGCCAGGCCCACGCCCCTCACCAGCACA; GCCTCGCCCGCAGCCACCACCCCGCTCCGCCGGGCACCCCTCACCACGCACCCAGTGGGTGCCATCAACC; AGCTGGGACCTGATCTGCCTCCAGCCACAGCCCCAGTCCCCAGCACCCGGCGGCCCCCAGCCCCGAATCT; ACACGTGTCCCCTGAGCTCTTCTGCGAGCCCCGAGAGGTACGGCGGGTCCAGTGGCCGGCCACCCAGCAG; GGCATGCTGGTGGAGAGGCCCTGCCCCAAGGGGACTCGAGGAATTGCCTCCTTCCAGTGTCTACCAGCCT; TGGGGCTCTGGAACCCCCGGGGCCCTGACCTCAGCAACTGCACCTCCCCCTGGGTCAACCAGGTGGCCCA; GAAGATCAAGAGTGGGGAGAACGCGGCCAACATCGCCAGCGAGCTGGCCCGACACACCCGGGGCTCCATC; TACGCGGGGGACGTCTCCTCCTCTGTGAAGCTGATGGAGCAGCTGCTGGACATCCTGGATGCCCAGCTGC; AGGCCCTGCGGCCCATCGAGCGCGAGTCAGCCGGCAAGAACTACAACAAGATGCACAAGCGAGAGAGAAC; TTGTAAGGATTATATCAAGGCCGTGGTGGAGACAGTGGACAATCTGCTCCGGCCAGAAGCTCTGGAGTCC; TGGAAGGACATGAATGCCACGGAGCAGGTGCACACGGCCACCATGCTCCTCGACGTCCTGGAGGAGGGCG; CCTTCCTGCTGGCCGACAATGTCAGGGAGCCTGCCCGCTTCCTGGCTGCCAAGGAGAACGTGGTCCTGGA; GGTCACAGTCCTGAACACAGAGGGCCAGGTGCAGGAGCTGGTGTTCCCCCACGAGGAGTACCCGAGAAAG; AACTCCATCCAGCTGTCTGCCAAAACCATCAAGCAGAACAGCCGCAATGGGGTGGTCAAAGTTGTCTTCA; TCCTCTACAACAACCTGGGCCTCTTCCTGTCCACGGAGAATGCCACAGTGAAGCTGGCCGGCGAAGCAGG; CCCGGGTGGCCCTGGGGGCGCCTCTCTAGTGGTGAACTCACAGGTCATCGCAGCATCCATCAACAAGGAG; TCCAGCCGCGTCTTCCTCATGGACCCTGTCATCTTCACCGTGGCCCACCTGGAGGACAAGAACCACTTCA; ATGCTAACTGCTCCTTCTGGAACTACTCGGAGCGTTCCATGCTGGGCTACTGGTCGACCCAAGGCTGCCG; CCTGGTGGAGTCCAACAAGACCCATACCACGTGTGCCTGCAGCCACCTCACCAACTTCGCTGTGCTCATG; GCTCACCGTGAGATCTACCAGGGCCGCATCAACGAGCTGCTGCTGTCGGTCATCACCTGGGTGGGCATTG; TGATCTCCCTGGTCTGCTTGGCCATCTGCATCTCCACCTTCTGCTTCCTGCGGGGGCTGCAGACCGACCG; CAACACCATCCACAAGAACCTGTGCATCAACCTCTTCCTGGCTGAGCTGCTCTTCCTGGTCGGGATCGAC; AAGACTCAGTATGAGATTGCCTGCCCCATCTTCGCCGGCCTGCTGCACTATTTCTTCCTGGCTGCCTTCT; CCTGGCTGTGCCTGGAGGGCGTGCACCTCTACCTGCTACTAGTGGAGGTGTTTGAGAGCGAGTATTCCCG; CACCAAGTACTACTACCTGGGTGGCTACTGCTTCCCGGCCCTGGTGGTGGGCATCGCGGCTGCCATTGAC; TACCGCAGCTACGGCACCGAGAAGGCCTGCTGGCTCCGAGTGGACAATTACTTCATCTGGAGTTTCATCG; GGCCAGTCTCCTTCGTTATCGTGGTCAACCTGGTGTTCCTCATGGTGACCCTGCACAAGATGATCCGAAG; CTCATCTGTGCTCAAGCCCGACTCCAGCCGCCTGGACAACATTAAATCCTGGGCGCTGGGGGCCATCGCG; CTGCTGTTCCTGCTGGGCCTCACCTGGGCTTTCGGCCTCCTCTTCATCAACAAGGAGTCGGTGGTCATGG; CCTATCTCTTCACCACCTTCAACGCCTTCCAGGGGGTCTTCATCTTCGTCTTTCACTGCGCCTTACAGAA; GAAGGTGCACAAGGAGTACAGCAAGTGCCTGCGTCACTCCTACTGCTGCATCCGCTCCCCACCCGGGGGC; ACTCACGGATCCCTCAAGACCTCAGCCATGCGAAGCAACACCCGCTACTACACAGGGACCCAGAGCCGAA; TTCGGAGGATGTGGAATGACACTGTGAGGAAACAGACGGAGTCCTCCTTCATGGCGGGTGACATCAACAG; CACCCCCACCCTGAACCGAGGTACCATGGGGAACCACCTGCTGACCAACCCCGTGCTGCAGCCCCGTGGG; GGCACCAGTCCCTACAACACCCTCATCGCCGAGTCAGTGGGCTTCAATCCCTCCTCGCCCCCTGTCTTCA; ACTCCCCAGGGAGCTACCGGGAACCCAAGCACCCCTTGGGAGGCCGGGAAGCCTGTGGCATGGACACCCT; GCCCCTGAACGGCAACTTCAATAACAGTTACTCCTTGCGAAGTGGGGATTTCCCTCCCGGGGATGGGGGC; CCTGAGCCGCCCCGAGGCCGGAACCTAGCCGATGCGGCGGCCTTTGAGAAGATGATCATCTCAGAGCTGG; TGCACAACAACCTGCGGGGGAGCAGCAGCGCGGCCAAGGGCCCTCCACCGCCTGAGCCCCCTGTGCCACC; TGTGCCAGGGGGCGGGGGCGAGGAAGAGGCGGGCGGGCCCGGGGGTGCTGACCGGGCCGAGATTGAACTT; CTCTATAAGGCCCTGGAGGAGCCTCTGCTGCTGCCCCGGGCCCAGTCGGTGCTGTACCAGAGCGATCTGG; ACGAGTCGGAGAGCTGCACGGCCGAGGACGGCGCCACCAGCCGGCCCCTCTCCTCCCCTCCTGGCCGGGA; CTCCCTCTATGCCAGCGGGGCCAACCTGCGGGACTCACCCTCCTACCCGGACAGCAGCCCTGAGGGGCCC; AGTGAGGCCCTGCCCCCACCCCCTCCCGCACCCCCCGGCCCCCCCGAAATCTACTACACCTCGCGCCCGC; CAGCCCTGGTGGCCCGGAATCCCCTGCAGGGCTACTACCAGGTGCGGCGTCCTAGCCACGAGGGCTACCT; GGCAGCCCCAGGCCTTGAGGGGCCAGGGCCCGATGGGGACGGGCAGATGCAGCTGGTCACCAGTCTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217296 representing NM_014921; Red=Cloning site Green=Tags(s); ; MARLAAVLWNLCVTAVLVTSATQGLSRAGLPFGLMRRELACEGYPIELRCPGSDVIMVENANYGRTDDKI; CDADPFQMENVQCYLPDAFKIMSQRCNNRTQCVVVAGSDAFPDPCPGTYKHLEVQYDCVPYIFVCPGTLQ; KVLEPTSTHESEHQSGAWCKDPLQAGDRIYVMPWIPYRTDTLTEYASWEDYVAARHTTTYRLPNRVDGTG; FVVYDGAVFYNKERTRNIVKYDLRTRIKSGETVINTANYHDTSPYRWGGKTDIDLAVDENGLWVIYATEG; NNGRLVVSQLNPYTLRFEGTWETGYDKRSASNAFMVCGVLYVLRSVYVDDDSEAAGNRVDYAFNTNANRE; EPVSLTFPNPYQFISSVDYNPRDNQLYVWNNYFVVRYSLEFGPPDPSAGPATSPPLSTTTTARPTPLTST; ASPAATTPLRRAPLTTHPVGAINQLGPDLPPATAPVPSTRRPPAPNLHVSPELFCEPREVRRVQWPATQQ; GMLVERPCPKGTRGIASFQCLPALGLWNPRGPDLSNCTSPWVNQVAQKIKSGENAANIASELARHTRGSI; YAGDVSSSVKLMEQLLDILDAQLQALRPIERESAGKNYNKMHKRERTCKDYIKAVVETVDNLLRPEALES; WKDMNATEQVHTATMLLDVLEEGAFLLADNVREPARFLAAKENVVLEVTVLNTEGQVQELVFPHEEYPRK; NSIQLSAKTIKQNSRNGVVKVVFILYNNLGLFLSTENATVKLAGEAGPGGPGGASLVVNSQVIAASINKE; SSRVFLMDPVIFTVAHLEDKNHFNANCSFWNYSERSMLGYWSTQGCRLVESNKTHTTCACSHLTNFAVLM; AHREIYQGRINELLLSVITWVGIVISLVCLAICISTFCFLRGLQTDRNTIHKNLCINLFLAELLFLVGID; KTQYEIACPIFAGLLHYFFLAAFSWLCLEGVHLYLLLVEVFESEYSRTKYYYLGGYCFPALVVGIAAAID; YRSYGTEKACWLRVDNYFIWSFIGPVSFVIVVNLVFLMVTLHKMIRSSSVLKPDSSRLDNIKSWALGAIA; LLFLLGLTWAFGLLFINKESVVMAYLFTTFNAFQGVFIFVFHCALQKKVHKEYSKCLRHSYCCIRSPPGG; THGSLKTSAMRSNTRYYTGTQSRIRRMWNDTVRKQTESSFMAGDINSTPTLNRGTMGNHLLTNPVLQPRG; GTSPYNTLIAESVGFNPSSPPVFNSPGSYREPKHPLGGREACGMDTLPLNGNFNNSYSLRSGDFPPGDGG; PEPPRGRNLADAAAFEKMIISELVHNNLRGSSSAAKGPPPPEPPVPPVPGGGGEEEAGGPGGADRAEIEL; LYKALEEPLLLPRAQSVLYQSDLDESESCTAEDGATSRPLSSPPGRDSLYASGANLRDSPSYPDSSPEGP; SEALPPPPPAPPGPPEIYYTSRPPALVARNPLQGYYQVRRPSHEGYLAAPGLEGPGPDGDGQMQLVTSL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014921
• ORF Size: 4407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014921.3, NP_055736.2
• RefSeq Size: 7871 bp
• RefSeq ORF: 4410 bp
• Locus ID: 22859
• UniProt ID: O94910
• Cytogenetics: 19p13.12
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene encodes a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species, endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. Latrophilin-1 has been shown to recruit the neurotoxin from black widow spider venom, alpha-latrotoxin, to the synapse plasma membrane. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]