RUFY1 (NM_025158) Human Tagged ORF Clone

SKU RG217181

RUFY1 (tGFP-tagged) - Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: RUFY1
• Synonyms: RABIP4; ZFYVE12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217181 representing NM_025158; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCGACCGGGAAGGCGGCTGCGCTGCTGGGCGGGGGCGGGAGCTGGAGCCGGAGCTGGAGCCGGGGC; CGGGGCCCGGGTCAGCGCTTGAGCCGGGAGAAGAGTTTGAGATCGTGGACCGAAGCCAGCTGCCCGGCCC; AGGCGACCTGCGGAGCGCAACGAGGCCGCGGGCGGCCGAGGGCTGGTCGGCGCCCATCCTGACCCTGGCA; CGCAGGGCCACCGGGAACCTGTCGGCGAGCTGCGGGAGCGCGCTGCGCGCGGCCGCGGGGCTGGGCGGCG; GGGACAGCGGGGACGGCACGGCGCGCGCAGCTTCTAAGTGCCAGATGATGGAGGAGCGTGCCAACCTGAT; GCACATGATGAAACTCAGCATCAAGGTGTTGCTCCAGTCGGCTCTGAGCCTGGGCCGCAGCCTGGATGCG; GACCATGCCCCCTTGCAGCAGTTCTTTGTAGTGATGGAGCACTGCCTCAAACATGGGCTGAAAGTTAAGA; AGAGTTTTATTGGCCAAAATAAATCATTCTTTGGTCCTTTGGAGCTGGTGGAGAAACTTTGTCCAGAAGC; ATCAGATATAGCGACTAGTGTCAGAAATCTTCCAGAATTAAAGACAGCTGTGGGAAGAGGCCGAGCGTGG; CTTTATCTTGCACTCATGCAAAAGAAACTGGCAGATTATCTGAAAGTGCTTATAGACAATAAACATCTCT; TAAGCGAGTTCTATGAGCCTGAGGCTTTAATGATGGAGGAAGAAGGGATGGTGATTGTTGGTCTGCTGGT; GGGACTCAATGTTCTCGATGCCAATCTCTGCTTGAAAGGAGAAGACTTGGATTCTCAGGTTGGAGTAATA; GATTTTTCCCTCTACCTTAAGGATGTGCAGGATCTTGATGGTGGCAAGGAGCATGAAAGAATTACTGATG; TCCTTGATCAAAAAAATTATGTGGAAGAACTTAACCGGCACTTGAGCTGCACAGTTGGGGATCTTCAAAC; CAAGATAGATGGCTTGGAAAAGACTAACTCAAAGCTTCAAGAAGAGCTTTCAGCTGCAACAGACCGAATT; TGCTCACTTCAAGAAGAACAGCAGCAGTTAAGAGAACAAAATGAATTAATTCGAGAAAGAAGTGAAAAGA; GTGTAGAGATAACAAAACAGGATACCAAAGTTGAGCTGGAGACTTACAAGCAAACTCGGCAAGGTCTGGA; TGAAATGTACAGTGATGTGTGGAAGCAGCTAAAAGAGGAGAAGAAAGTCCGGTTGGAACTGGAAAAAGAA; CTGGAGTTACAAATTGGAATGAAAACCGAAATGGAAATTGCAATGAAGTTACTGGAAAAGGACACCCACG; AGAAGCAGGACACACTAGTTGCCCTCCGCCAGCAGCTGGAAGAAGTCAAAGCGATTAATTTACAGATGTT; TCACAAAGCTCAGAATGCAGAGAGCAGTTTGCAGCAGAAGAATGAAGCCATCACATCCTTTGAAGGAAAA; ACCAACCAAGTTATGTCCAGCATGAAACAAATGGAAGAAAGGTTGCAGCACTCGGAGCGGGCGAGGCAGG; GGGCTGAGGAGCGGAGCCACAAGCTGCAGCAGGAGCTGGGCGGGAGGATCGGCGCCCTGCAGCTGCAGCT; CTCCCAGCTGCACGAGCAATGCTCAAGCCTGGAGAAAGAATTGAAATCAGAAAAAGAGCAAAGACAGGCT; CTTCAGCGCGAATTACAGCACGAGAAAGACACTTCCTCTCTACTCAGGATGGAGCTGCAACAAGTGGAAG; GACTGAAAAAGGAGTTGCGGGAGCTTCAGGACGAGAAGGCAGAGCTGCAGAAGATCTGCGAGGAGCAGGA; ACAAGCCCTCCAGGAAATGGGCCTGCACCTCAGCCAGTCCAAGCTGAAGATGGAAGATATAAAAGAAGTG; AACCAGGCACTGAAGGGCCACGCCTGGCTGAAAGATGACGAAGCGACACACTGTAGGCAGTGTGAGAAGG; AGTTCTCCATTTCCCGGAGAAAGCACCACTGCCGGAACTGTGGCCACATCTTCTGCAACACCTGCTCCAG; CAACGAGCTGGCCCTGCCCTCCTACCCCAAGCCGGTGCGAGTGTGCGACAGCTGCCACACCCTGCTCCTG; CAGCGCTGCTCCTCCACGGCCTCC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217181 representing NM_025158; Red=Cloning site Green=Tags(s); ; MADREGGCAAGRGRELEPELEPGPGPGSALEPGEEFEIVDRSQLPGPGDLRSATRPRAAEGWSAPILTLA; RRATGNLSASCGSALRAAAGLGGGDSGDGTARAASKCQMMEERANLMHMMKLSIKVLLQSALSLGRSLDA; DHAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAW; LYLALMQKKLADYLKVLIDNKHLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVI; DFSLYLKDVQDLDGGKEHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAATDRI; CSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKE; LELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKAQNAESSLQQKNEAITSFEGK; TNQVMSSMKQMEERLQHSERARQGAEERSHKLQQELGGRIGALQLQLSQLHEQCSSLEKELKSEKEQRQA; LQRELQHEKDTSSLLRMELQQVEGLKKELRELQDEKAELQKICEEQEQALQEMGLHLSQSKLKMEDIKEV; NQALKGHAWLKDDEATHCRQCEKEFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLL; QRCSSTAS; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025158
• ORF Size: 2124 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_025158.5
• RefSeq Size: 2644 bp
• RefSeq ORF: 2127 bp
• Locus ID: 80230
• UniProt ID: Q96T51
• Cytogenetics: 5q35.3
• Domains: FYVE, RUN
• Protein Pathways: Endocytosis
• Summary: This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]