CD79B (NM_000626) Human Tagged ORF Clone

  • TrueORF®
SKU
RG215340
CD79B (tGFP-tagged) - Human CD79b molecule, immunoglobulin-associated beta (CD79B), transcript variant 1
  $650.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD79B
Synonyms AGM6; B29; IGB
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG215340 representing NM_000626
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGGCTGGCGTTGTCTCCTGTGCCCAGCCACTGGATGGTGGCGTTGCTGCTGCTGCTCTCAGCTG
AGCCAGTACCAGCAGCCAGATCGGAGGACCGGTACCGGAATCCCAAAGGTAGTGCTTGTTCGCGGATCTG
GCAGAGCCCACGTTTCATAGCCAGGAAACGGGGCTTCACGGTGAAAATGCACTGCTACATGAACAGCGCC
TCCGGCAATGTGAGCTGGCTCTGGAAGCAGGAGATGGACGAGAATCCCCAGCAGCTGAAGCTGGAAAAGG
GCCGCATGGAAGAGTCCCAGAACGAATCTCTCGCCACCCTCACCATCCAAGGCATCCGGTTTGAGGACAA
TGGCATCTACTTCTGCCAGCAGAAGTGCAACAACACCTCGGAGGTCTACCAGGGCTGCGGCACAGAGCTG
CGAGTCATGGGATTCAGCACCTTGGCACAGCTGAAGCAGAGGAACACGCTGAAGGATGGTATCATCATGA
TCCAGACGCTGCTGATCATCCTCTTCATCATCGTGCCTATCTTCCTGCTGCTGGACAAGGATGACAGCAA
GGCTGGCATGGAGGAAGATCACACCTACGAGGGCCTGGACATTGACCAGACAGCCACCTATGAGGACATA
GTGACGCTGCGGACAGGGGAAGTGAAGTGGTCTGTAGGTGAGCACCCAGGCCAGGAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG215340 representing NM_000626
Red=Cloning site Green=Tags(s)

MARLALSPVPSHWMVALLLLLSAEPVPAARSEDRYRNPKGSACSRIWQSPRFIARKRGFTVKMHCYMNSA
SGNVSWLWKQEMDENPQQLKLEKGRMEESQNESLATLTIQGIRFEDNGIYFCQQKCNNTSEVYQGCGTEL
RVMGFSTLAQLKQRNTLKDGIIMIQTLLIILFIIVPIFLLLDKDDSKAGMEEDHTYEGLDIDQTATYEDI
VTLRTGEVKWSVGEHPGQE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000626
ORF Size 687 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000626.1, NP_000617.1
RefSeq Size 1270 bp
RefSeq ORF 690 bp
Locus ID 974
UniProt ID P40259
Cytogenetics 17q23.3
Protein Families Druggable Genome, Transmembrane
Protein Pathways B cell receptor signaling pathway
Summary The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_000626" in other vectors (6)
SKU Description Size Price
RC215340 CD79B (Myc-DDK-tagged)-Human CD79b molecule, immunoglobulin-associated beta (CD79B), transcript variant 1 10 ug
$450.00
RC215340L1 Lenti ORF clone of Human CD79b molecule, immunoglobulin-associated beta (CD79B), transcript variant 1, Myc-DDK-tagged 10 ug
$750.00
RC215340L2 Lenti ORF clone of Human CD79b molecule, immunoglobulin-associated beta (CD79B), transcript variant 1, mGFP tagged 10 ug
$750.00
RC215340L3 Lenti ORF clone of Human CD79b molecule, immunoglobulin-associated beta (CD79B), transcript variant 1, Myc-DDK-tagged 10 ug
$750.00
RC215340L4 Lenti ORF clone of Human CD79b molecule, immunoglobulin-associated beta (CD79B), transcript variant 1, mGFP tagged 10 ug
$750.00
SC122570 CD79B (untagged)-Human CD79b molecule, immunoglobulin-associated beta (CD79B), transcript variant 1 10 ug
$450.00

Plasmid Map for RG215340

Download sequence for

Cloning scheme for RG215340

Circular map for RG215340

file-alt Citations

CD79B limits response of diffuse large B cell lymphoma to ibrutinib
Kim, Kim, Ryu et al
Leuk. Lymphoma (2015)
Cited by 44
AI Summary: OriGene's CD79B expression plasmid (pCMV-AC-GFP + CD79B) was used in this study to overexpress CD79B and demonstrate its role in conferring ibrutinib resistance in diffuse large B-cell lymphoma.
Snippet: Ibt) for 4 weeks. Expression plasmids encoding wild-type CD79B (pCMV-AC-GFP + CD79B) were purchased from OriGene (Rockville, MD 20850) and CD79B RNAi from Qiagen (Hilden, Germany). GeneSolution siRNAs used ... Cite SaveSaving
Showing 1-1 of 1 papers.
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