DNA polymerase alpha (POLA1) (NM_016937) Human Tagged ORF Clone

SKU RG214011

POLA1 (tGFP-tagged) - Human polymerase (DNA directed), alpha 1, catalytic subunit (POLA1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DNA polymerase alpha
• Synonyms: NSX; p180; POLA; VEODS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214011 representing NM_016937; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCACCTGTGCACGGCGACGACTCTCTGTCAGATTCAGGGAGTTTTGTATCTTCTCGAGCCCGGCGAG; AAAAAAAATCAAAGAAGGGGCGCCAAGAAGCCCTAGAAAGACTGAAAAAGGCTAAAGCTGGTGAGAAGTA; TAAATATGAAGTCGAGGACTTCACAGGTGTTTATGAAGAAGTTGATGAAGAACAGTATTCGAAGCTGGTT; CAGGCACGCCAGGATGATGACTGGATTGTGGATGATGATGGTATTGGCTATGTGGAAGATGGCCGAGAGA; TTTTTGATGATGACCTTGAAGATGATGCCCTTGATGCTGATGAGAAAGGAAAAGATGGTAAAGCACGCAA; TAAAGACAAGAGGAATGTAAAGAAGCTCGCAGTGACAAAACCGAACAACATTAAGTCAATGTTCATTGCT; TGTGCTGGAAAGAAAACTGCAGATAAAGCTGTAGACTTGTCCAAGGATGGTCTGCTAGGTGACATTCTAC; AGGATCTTAACACTGAGACACCTCAAATAACTCCACCACCTGTAATGATACTGAAGAAGAAAAGATCCAT; TGGAGCTTCACCGAATCCTTTCTCTGTGCACACCGCCACGGCAGTTCCTTCAGGAAAAATTGCTTCCCCT; GTCTCCAGAAAGGAGCCTCCATTAACTCCTGTTCCTCTTAAACGTGCTGAATTTGCTGGCGATGATGTAC; AGGTCGAGAGTACAGAAGAAGAGCAGGAGTCAGGGGCAATGGAGTTTGAAGATGGTGACTTTGATGAGCC; CATGGAAGTTGAAGAGGTGGACCTGGAGCCTATGGCTGCCAAGGCTTGGGACAAAGAGAGTGAGCCAGCA; GAGGAAGTGAAACAAGAGGCGGATTCTGGGAAAGGGACCGTGTCCTACTTAGGAAGTTTTCTCCCGGATG; TCTCTTGTTGGGACATTGATCAAGAAGGTGATAGCAGTTTCTCAGTGCAAGAAGTTCAAGTGGATTCCAG; TCACCTCCCATTGGTAAAAGGGGCAGATGAGGAACAAGTATTCCACTTTTATTGGTTGGATGCTTATGAG; GATCAGTACAACCAACCAGGTGTGGTATTTCTGTTTGGGAAAGTTTGGATTGAATCAGCCGAGACCCATG; TGAGCTGTTGTGTCATGGTGAAAAATATCGAGCGAACGCTTTACTTCCTTCCCCGTGAAATGAAAATTGA; TCTAAATACGGGGAAAGAAACAGGAACTCCAATTTCAATGAAGGATGTTTATGAGGAATTTGATGAGAAA; ATAGCAACAAAATATAAAATTATGAAGTTCAAGTCTAAGCCAGTGGAAAAGAACTATGCTTTTGAGATAC; CTGATGTTCCAGAAAAATCTGAGTACTTGGAAGTTAAATACTCGGCTGAAATGCCACAGCTTCCTCAAGA; TTTGAAAGGAGAAACTTTTTCTCATGTATTTGGGACCAACACATCTAGCCTGGAACTGTTCTTGATGAAC; AGAAAGATCAAAGGACCTTGTTGGCTTGAAGTAAAAAAGTCCACAGCTCTTAATCAGCCAGTCAGTTGGT; GTAAAGTTGAGGCAATGGCTTTGAAACCAGACCTGGTGAATGTAATTAAGGATGTCAGTCCACCACCGCT; TGTCGTGATGGCTTTCAGCATGAAGACAATGCAGAATGCAAAGAACCATCAAAATGAGATTATTGCTATG; GCAGCTTTGGTCCATCACAGTTTTGCATTGGATAAAGCAGCCCCAAAGCCTCCCTTTCAGTCACACTTCT; GTGTTGTGTCTAAACCAAAGGACTGTATTTTTCCATATGCTTTCAAAGAAGTCATTGAGAAAAAGAATGT; GAAGGTTGAGGTTGCTGCAACAGAAAGAACACTGCTAGGTTTTTTCCTTGCAAAAGTTCACAAAATTGAT; CCTGATATCATTGTGGGTCATAATATTTATGGGTTTGAACTGGAAGTACTACTGCAGAGAATTAATGTGT; GCAAAGCTCCTCACTGGTCCAAGATAGGTCGACTGAAGCGATCCAACATGCCAAAGCTTGGGGGCCGGAG; TGGATTTGGTGAAAGAAATGCTACCTGTGGTCGAATGATCTGTGATGTGGAAATTTCAGCAAAGGAATTG; ATTCGTTGTAAAAGCTACCATCTGTCTGAACTTGTTCAGCAGATTCTAAAAACTGAAAGGGTTGTAATCC; CAATGGAAAATATACAAAATATGTACAGTGAATCTTCTCAACTGTTATACCTGTTGGAACACACCTGGAA; AGATGCCAAGTTCATTTTGCAGATCATGTGTGAGCTAAATGTTCTTCCATTAGCATTGCAGATCACTAAC; ATCGCTGGGAACATTATGTCCAGGACGCTGATGGGTGGACGATCCGAGCGTAACGAGTTCTTGTTGCTTC; ATGCATTTTACGAAAACAACTATATTGTGCCTGACAAGCAGATTTTCAGAAAGCCTCAGCAAAAACTGGG; AGATGAAGATGAAGAAATTGATGGAGATACCAATAAATACAAGAAAGGACGTAAGAAAGGAGCTTATGCT; GGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGATAAGTTCATTTTGCTTCTGGACTTCAACAGTC; TATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGTACAAAGAGTTGCTTCAGAGGCACA; GAAAGTTACAGAGGATGGAGAACAAGAACAGATCCCTGAGTTGCCAGATCCAAGCTTAGAAATGGGCATT; TTGCCCAGAGAGATCCGGAAACTGGTAGAACGGAGAAAACAAGTCAAACAGCTAATGAAACAGCAAGACT; TAAATCCAGACCTTATTCTTCAGTATGACATTCGACAGAAGGCTTTGAAGCTCACAGCGAACAGTATGTA; TGGTTGCCTGGGATTTTCCTATAGCAGATTTTACGCCAAACCACTGGCTGCCTTGGTGACATACAAAGGA; AGGGAGATTTTGATGCATACGAAAGAGATGGTACAAAAGATGAATCTTGAAGTTATTTATGGAGATACAG; ATTCAATTATGATAAACACCAATAGCACCAATCTGGAAGAAGTATTTAAGTTGGGAAACAAGGTAAAAAG; TGAAGTGAATAAGTTGTACAAACTGCTTGAAATAGACATTGATGGGGTTTTCAAGTCTCTGCTACTGCTG; AAAAAAAAGAAGTACGCTGCTCTGGTTGTTGAGCCAACGTCGGATGGGAATTATGTCACCAAACAGGAGC; TCAAAGGATTAGATATAGTTAGAAGAGATTGGTGTGATCTTGCTAAAGACACTGGAAACTTTGTGATTGG; CCAGATTCTTTCTGATCAAAGCCGGGACACTATAGTGGAAAACATTCAGAAGAGGCTGATAGAAATTGGA; GAAAATGTGCTAAATGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACAAGGCATTGACAAAGGATCCCC; AGGATTACCCTGATAAAAAAAGCCTACCTCATGTACATGTTGCCCTCTGGATAAATTCTCAAGGAGGCAG; AAAGGTGAAAGCTGGAGATACTGTGTCATATGTCATCTGTCAGGATGGATCAAACCTCACTGCAAGTCAG; AGGGCCTATGCGCCTGAGCAGCTGCAGAAACAGGATAATCTAACCATTGACACCCAGTACTACCTGGCCC; AGCAGATCCACCCAGTCGTGGCTCGGATCTGTGAACCAATAGACGGAATTGATGCTGTCCTCATTGCAAC; GTGGTTGGGACTTGACCCCACCCAATTTAGAGTTCATCATTATCATAAAGATGAAGAGAATGATGCTCTA; CTTGGTGGCCCAGCACAGCTCACTGATGAAGAGAAATACAGGGACTGTGAAAGATTCAAATGTCCATGCC; CTACATGTGGAACTGAGAATATTTATGATAATGTCTTTGATGGTTCGGGAACAGATATGGAGCCCAGCTT; GTATCGTTGCAGTAACATCGATTGTAAGGCTTCACCTCTGACCTTTACAGTACAACTGAGCAACAAATTG; ATCATGGACATTAGACGTTTCATTAAAAAGTACTATGATGGCTGGTTGATATGTGAAGAGCCAACCTGTC; GCAATCGAACTCGTCACCTTCCCCTTCAATTCTCCCGAACTGGGCCTCTTTGCCCAGCCTGCATGAAAGC; TACACTTCAACCAGAGTATTCTGACAAGTCCCTGTACACCCAGCTGTGCTTTTACCGGTACATTTTTGAT; GCGGAGTGTGCACTGGAGAAACTTACTACCGATCATGAGAAAGATAAATTGAAGAAGCAATTTTTTACCC; CCAAAGTTCTGCAGGACTACAGAAAACTCAAGAACACAGCAGAGCAATTCTTGTCCCGAAGTGGCTACTC; CGAAGTGAATCTGAGCAAACTCTTCGCTGGTTGTGCCGTGAAATCC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214011 representing NM_016937; Red=Cloning site Green=Tags(s); ; MAPVHGDDSLSDSGSFVSSRARREKKSKKGRQEALERLKKAKAGEKYKYEVEDFTGVYEEVDEEQYSKLV; QARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDADEKGKDGKARNKDKRNVKKLAVTKPNNIKSMFIA; CAGKKTADKAVDLSKDGLLGDILQDLNTETPQITPPPVMILKKKRSIGASPNPFSVHTATAVPSGKIASP; VSRKEPPLTPVPLKRAEFAGDDVQVESTEEEQESGAMEFEDGDFDEPMEVEEVDLEPMAAKAWDKESEPA; EEVKQEADSGKGTVSYLGSFLPDVSCWDIDQEGDSSFSVQEVQVDSSHLPLVKGADEEQVFHFYWLDAYE; DQYNQPGVVFLFGKVWIESAETHVSCCVMVKNIERTLYFLPREMKIDLNTGKETGTPISMKDVYEEFDEK; IATKYKIMKFKSKPVEKNYAFEIPDVPEKSEYLEVKYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMN; RKIKGPCWLEVKKSTALNQPVSWCKVEAMALKPDLVNVIKDVSPPPLVVMAFSMKTMQNAKNHQNEIIAM; AALVHHSFALDKAAPKPPFQSHFCVVSKPKDCIFPYAFKEVIEKKNVKVEVAATERTLLGFFLAKVHKID; PDIIVGHNIYGFELEVLLQRINVCKAPHWSKIGRLKRSNMPKLGGRSGFGERNATCGRMICDVEISAKEL; IRCKSYHLSELVQQILKTERVVIPMENIQNMYSESSQLLYLLEHTWKDAKFILQIMCELNVLPLALQITN; IAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKGAYA; GGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVTEDGEQEQIPELPDPSLEMGI; LPREIRKLVERRKQVKQLMKQQDLNPDLILQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKG; REILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGVFKSLLLL; KKKKYAALVVEPTSDGNYVTKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIG; ENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTASQ; RAYAPEQLQKQDNLTIDTQYYLAQQIHPVVARICEPIDGIDAVLIATWLGLDPTQFRVHHYHKDEENDAL; LGGPAQLTDEEKYRDCERFKCPCPTCGTENIYDNVFDGSGTDMEPSLYRCSNIDCKASPLTFTVQLSNKL; IMDIRRFIKKYYDGWLICEEPTCRNRTRHLPLQFSRTGPLCPACMKATLQPEYSDKSLYTQLCFYRYIFD; AECALEKLTTDHEKDKLKKQFFTPKVLQDYRKLKNTAEQFLSRSGYSEVNLSKLFAGCAVKS; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016937
• ORF Size: 4386 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016937.1, NP_058633.1
• RefSeq Size: 5433 bp
• RefSeq ORF: 4389 bp
• Locus ID: 5422
• UniProt ID: P09884
• Cytogenetics: Xp22.11-p21.3
• Protein Families: Druggable Genome
• Protein Pathways: DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• Summary: This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010]