ABCB4 (NM_018850) Human Tagged ORF Clone

SKU RG212987

ABCB4 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant C

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ABCB4
• Synonyms: ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3; PFIC-3; PGY3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212987 representing NM_018850; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATCTTGAGGCGGCAAAGAACGGAACAGCCTGGCGCCCCACGAGCGCGGAGGGCGACTTTGAACTGG; GCATCAGCAGCAAACAAAAAAGGAAAAAAACGAAGACAGTGAAAATGATTGGAGTATTAACATTGTTTCG; ATACTCCGATTGGCAGGATAAATTGTTTATGTCGCTGGGTACCATCATGGCCATAGCTCACGGATCAGGT; CTCCCCCTCATGATGATAGTATTTGGAGAGATGACTGACAAATTTGTTGATACTGCAGGAAACTTCTCCT; TTCCAGTGAACTTTTCCTTGTCGCTGCTAAATCCAGGCAAAATTCTGGAAGAAGAAATGACTAGATATGC; ATATTACTACTCAGGATTGGGTGCTGGAGTTCTTGTTGCTGCCTATATACAAGTTTCATTTTGGACTTTG; GCAGCTGGTCGACAGATCAGGAAAATTAGGCAGAAGTTTTTTCATGCTATTCTACGACAGGAAATAGGAT; GGTTTGACATCAACGACACCACTGAACTCAATACGCGGCTAACAGATGACATCTCCAAAATCAGTGAAGG; AATTGGTGACAAGGTTGGAATGTTCTTTCAAGCAGTAGCCACGTTTTTTGCAGGATTCATAGTGGGATTC; ATCAGAGGATGGAAGCTCACCCTTGTGATAATGGCCATCAGCCCTATTCTAGGACTCTCTGCAGCCGTTT; GGGCAAAGATACTCTCGGCATTTAGTGACAAAGAACTAGCTGCTTATGCAAAAGCAGGCGCCGTGGCAGA; AGAGGCTCTGGGGGCCATCAGGACTGTGATAGCTTTCGGGGGCCAGAACAAAGAGCTGGAAAGGTATCAG; AAACATTTAGAAAATGCCAAAGAGATTGGAATTAAAAAAGCTATTTCAGCAAACATTTCCATGGGTATTG; CCTTCCTGTTAATATATGCATCATATGCACTGGCCTTCTGGTATGGATCCACTCTAGTCATATCAAAAGA; ATATACTATTGGAAATGCAATGACAGTTTTTTTTTCAATCCTAATTGGAGCTTTCAGTGTTGGCCAGGCT; GCCCCATGTATTGATGCTTTTGCCAATGCAAGAGGAGCAGCATATGTGATCTTTGATATTATTGATAATA; ATCCTAAAATTGACAGTTTTTCAGAGAGAGGACACAAACCAGACAGCATCAAAGGGAATTTGGAGTTCAA; TGATGTTCACTTTTCTTACCCTTCTCGAGCTAACGTCAAGATCTTGAAGGGCCTCAACCTGAAGGTGCAG; AGTGGGCAGACGGTGGCCCTGGTTGGAAGTAGTGGCTGTGGGAAGAGCACAACGGTCCAGCTGATACAGA; GGCTCTATGACCCTGATGAGGGCACAATTAACATTGATGGGCAGGATATTAGGAACTTTAATGTAAACTA; TCTGAGGGAAATCATTGGTGTGGTGAGTCAGGAGCCGGTGCTGTTTTCCACCACAATTGCTGAAAATATT; TGTTATGGCCGTGGAAATGTAACCATGGATGAGATAAAGAAAGCTGTCAAAGAGGCCAACGCCTATGAGT; TTATCATGAAATTACCACAGAAATTTGACACCCTGGTTGGAGAGAGAGGGGCCCAGCTGAGTGGTGGGCA; GAAGCAGAGGATCGCCATTGCACGTGCCCTGGTTCGCAACCCCAAGATCCTTCTGCTGGATGAGGCCACG; TCAGCATTGGACACAGAAAGTGAAGCTGAGGTACAGGCAGCTCTGGATAAGGCCAGAGAAGGCCGGACCA; CCATTGTGATAGCACACCGACTGTCTACGGTCCGAAATGCAGATGTCATCGCTGGGTTTGAGGATGGAGT; AATTGTGGAGCAAGGAAGCCACAGCGAACTGATGAAGAAGGAAGGGGTGTACTTCAAACTTGTCAACATG; CAGACATCAGGAAGCCAGATCCAGTCAGAAGAATTTGAACTAAATGATGAAAAGGCTGCCACTAGAATGG; CCCCAAATGGCTGGAAATCTCGCCTATTTAGGCATTCTACTCAGAAAAACCTTAAAAATTCACAAATGTG; TCAGAAGAGCCTTGATGTGGAAACCGATGGACTTGAAGCAAATGTGCCACCAGTGTCCTTTCTGAAGGTC; CTGAAACTGAATAAAACAGAATGGCCCTACTTTGTCGTGGGAACAGTATGTGCCATTGCCAATGGGGGGC; TTCAGCCGGCATTTTCAGTCATATTCTCAGAGATCATAGCGATTTTTGGACCAGGCGATGATGCAGTGAA; GCAGCAGAAGTGCAACATATTCTCTTTGATTTTCTTATTTCTGGGAATTATTTCTTTTTTTACTTTCTTC; CTTCAGGGTTTCACGTTTGGGAAAGCTGGCGAGATCCTCACCAGAAGACTGCGGTCAATGGCTTTTAAAG; CAATGCTAAGACAGGACATGAGCTGGTTTGATGACCATAAAAACAGTACTGGTGCACTTTCTACAAGACT; TGCCACAGATGCTGCCCAAGTCCAAGGAGCCACAGGAACCAGGTTGGCTTTAATTGCACAGAATATAGCT; AACCTTGGAACTGGTATTATCATATCATTTATCTACGGTTGGCAGTTAACCCTATTGCTATTAGCAGTTG; TTCCAATTATTGCTGTGTCAGGAATTGTTGAAATGAAATTGTTGGCTGGAAATGCCAAAAGAGATAAAAA; AGAACTGGAAGCTGCTGGAAAGATTGCAACAGAGGCAATAGAAAATATTAGGACAGTTGTGTCTTTGACC; CAGGAAAGAAAATTTGAATCAATGTATGTTGAAAAATTGTATGGACCTTACAGGGTGTTTTCTGCAATTG; TATTTGGTGCAGTGGCTCTAGGACATGCCAGTTCATTTGCTCCAGACTATGCTAAAGCTAAGCTGTCTGC; AGCCCACTTATTCATGCTGTTTGAAAGACAACCTCTGATTGACAGCTACAGTGAAGAGGGGCTGAAGCCT; GATAAATTTGAAGGAAATATAACATTTAATGAAGTCGTGTTCAACTATCCCACCCGAGCAAACGTGCCAG; TGCTTCAGGGGCTGAGCCTGGAGGTGAAGAAAGGCCAGACACTAGCCCTGGTGGGCAGCAGTGGCTGTGG; GAAGAGCACGGTGGTCCAGCTCCTGGAGCGGTTCTACGACCCCTTGGCGGGGACAGTGCTTCTCGATGGT; CAAGAAGCAAAGAAACTCAATGTCCAGTGGCTCAGAGCTCAACTCGGAATCGTGTCTCAGGAGCCTATCC; TATTTGACTGCAGCATTGCCGAGAATATTGCCTATGGAGACAACAGCCGGGTTGTATCACAGGATGAAAT; TGTGAGTGCAGCCAAAGCTGCCAACATACATCCTTTCATCGAGACGTTACCCCACAAATATGAAACAAGA; GTGGGAGATAAGGGGACTCAGCTCTCAGGAGGTCAAAAACAGAGGATTGCTATTGCCCGAGCCCTCATCA; GACAACCTCAAATCCTCCTGTTGGATGAAGCTACATCAGCTCTGGATACTGAAAGTGAAAAGGTTGTCCA; AGAAGCCCTGGACAAAGCCAGAGAAGGCCGCACCTGCATTGTGATTGCTCACCGCCTGTCCACCATCCAG; AATGCAGACTTAATAGTGGTGTTTCAGAATGGGAGAGTCAAGGAGCATGGCACGCATCAGCAGCTGCTGG; CACAGAAAGGCATCTATTTTTCAATGGTCAGTGTCCAGGCTGGGACACAGAACTTA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212987 representing NM_018850; Red=Cloning site Green=Tags(s); ; MDLEAAKNGTAWRPTSAEGDFELGISSKQKRKKTKTVKMIGVLTLFRYSDWQDKLFMSLGTIMAIAHGSG; LPLMMIVFGEMTDKFVDTAGNFSFPVNFSLSLLNPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTL; AAGRQIRKIRQKFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGF; IRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQ; KHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQA; APCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQ; SGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENI; CYGRGNVTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEAT; SALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNM; QTSGSQIQSEEFELNDEKAATRMAPNGWKSRLFRHSTQKNLKNSQMCQKSLDVETDGLEANVPPVSFLKV; LKLNKTEWPYFVVGTVCAIANGGLQPAFSVIFSEIIAIFGPGDDAVKQQKCNIFSLIFLFLGIISFFTFF; LQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIA; NLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLT; QERKFESMYVEKLYGPYRVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKP; DKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVLLDG; QEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETR; VGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQ; NADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTQNL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018850
• ORF Size: 3696 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018850.2, NP_061338.1
• RefSeq Size: 3826 bp
• RefSeq ORF: 3699 bp
• Locus ID: 5244
• UniProt ID: P21439
• Cytogenetics: 7q21.12
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008]