DNA Polymerase theta (POLQ) (NM_199420) Human Tagged ORF Clone

SKU RG212566

POLQ (tGFP-tagged) - Human polymerase (DNA directed), theta (POLQ)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DNA Polymerase theta
• Synonyms: PRO0327
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212566 representing NM_199420; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAATCTTCTGCGTCGGAGTGGGAAACGGCGGCGTTCAGAATCAGGCTCAGATTCGTTCTCGGGAAGCG; GCGGTGACAGCAGTGCCAGCCCCCAGTTCCTCTCCGGGTCCGTGCTGAGCCCGCCGCCCGGCCTTGGTCG; CTGCCTGAAGGCCGCAGCTGCAGGAGAATGCAAGCCTACAGTTCCTGACTACGAAATAGACAAGCTACTA; TTGGCAAACTGGGGACTTCCTAAAGCAGTTCTGGAAAAATACCACAGTTTTGGTGTAAAAAAGATGTTTG; AATGGCAGGCAGAGTGCCTTTTGCTTGGACAAGTCCTGGAAGGAAAGAATTTAGTTTATTCAGCTCCTAC; AAGTGCTGGGAAGACTCTTGTGGCAGAATTACTTATTTTGAAGCGGGTTTTGGAAATGCGGAAGAAAGCT; TTGTTTATTCTTCCCTTTGTTTCTGTGGCTAAAGAGAAGAAATACTACCTCCAGAGTCTGTTTCAGGAAG; TAGGAATAAAAGTAGACGGTTATATGGGCAGCACCTCTCCATCAAGGCATTTCTCTTCATTGGATATTGC; AGTCTGCACAATTGAGAGAGCCAATGGTCTGATCAATCGCCTCATAGAGGAAAATAAGATGGATCTGTTA; GGAATGGTGGTTGTGGATGAATTACATATGCTGGGAGACTCTCACCGAGGGTATCTGCTGGAACTTTTGC; TGACCAAGATTTGCTATATTACTCGGAAATCAGCATCTTGTCAGGCAGATCTAGCCAGTTCTCTGTCTAA; TGCTGTGCAAATCGTTGGCATGAGTGCTACCCTTCCTAATTTGGAGCTTGTGGCTTCCTGGTTGAATGCT; GAACTCTACCATACCGACTTTCGCCCTGTACCGCTTTTGGAGTCAGTAAAAGTTGGAAATTCCATATATG; ACTCTTCAATGAAACTTGTGAGGGAATTTGAGCCCATGCTACAAGTGAAGGGAGATGAGGACCATGTTGT; TAGTTTATGTTATGAGACGATTTGTGATAACCATTCAGTATTACTTTTTTGTCCATCAAAGAAATGGTGT; GAGAAGCTGGCAGATATCATTGCTCGAGAGTTTTATAATCTACATCATCAAGCTGAGGGATTGGTGAAAC; CCTCTGAATGCCCACCAGTAATTCTGGAACAAAAAGAACTCCTGGAAGTGATGGATCAGTTAAGACGTTT; GCCTTCAGGACTGGACTCTGTATTACAGAAAACTGTACCATGGGGAGTAGCATTTCATCATGCAGGTCTT; ACTTTTGAGGAGAGGGATATCATTGAAGGAGCCTTTCGTCAAGGTCTCATTCGGGTCTTGGCGGCAACTT; CTACTCTTTCTTCTGGGGTGAATTTACCTGCACGTCGTGTGATTATTCGAACCCCTATTTTTGGTGGTCG; ACCTCTAGATATTCTTACTTATAAGCAGATGGTTGGCCGTGCTGGCAGGAAAGGAGTGGACACAGTAGGC; GAGAGTATCTTAATTTGTAAGAACTCTGAGAAATCAAAAGGCATAGCTCTCCTTCAGGGTTCTCTAAAGC; CTGTTCGCAGCTGTCTGCAAAGACGAGAAGGAGAAGAAGTAACTGGCAGCATGATACGAGCTATTCTGGA; GATAATAGTTGGTGGAGTGGCAAGTACATCACAAGATATGCATACTTATGCTGCCTGCACATTTTTGGCT; GCAAGTATGAAAGAAGGGAAGCAAGGAATTCAGAGAAATCAAGAGTCTGTTCAGCTTGGAGCGATTGAGG; CCTGTGTGATGTGGCTACTAGAAAATGAATTCATCCAGAGTACAGAAGCCAGTGATGGAACAGAAGGAAA; GGTGTATCATCCAACACATCTTGGTTCGGCCACTCTTTCTTCTTCACTTTCTCCAGCTGATACTTTAGAT; ATTTTTGCTGACCTGCAAAGAGCAATGAAGGGCTTTGTTTTAGAGAATGATCTTCATATTCTCTATCTGG; TTACACCTATGTTTGAGGATTGGACTACTATTGATTGGTATCGATTTTTCTGTTTATGGGAGAAGTTGCC; AACTTCAATGAAAAGGGTGGCAGAGCTAGTGGGAGTTGAAGAGGGGTTCTTGGCCCGTTGTGTGAAAGGA; AAAGTAGTAGCCAGAACTGAGAGACAGCATCGACAAATGGCCATCCATAAAAGGTTTTTCACCAGTCTTG; TGCTATTAGATTTAATCAGTGAAGTTCCCTTAAGGGAAATAAATCAGAAATATGGATGCAATCGTGGGCA; GATTCAATCTTTGCAACAGTCAGCTGCTGTTTATGCAGGGATGATTACAGTATTTTCCAACCGTCTGGGC; TGGCACAACATGGAACTACTACTTTCCCAATTTCAGAAGCGTCTTACGTTTGGCATCCAGAGGGAGCTGT; GTGACCTGGTTCGGGTATCCTTACTAAATGCTCAGAGAGCCAGGGTTCTCTATGCTTCTGGCTTTCATAC; TGTGGCAGACCTTGCTAGAGCAAATATTGTGGAGGTGGAGGTGATTCTGAAAAATGCTGTGCCTTTCAAA; AGTGCCCGGAAGGCAGTGGATGAGGAAGAGGAAGCAGTTGAAGAACGTCGCAATATGCGAACTATCTGGG; TGACTGGCAGAAAAGGTTTAACTGAAAGGGAAGCAGCAGCCCTTATAGTGGAAGAAGCCAGAATGATTCT; GCAGCAGGACTTAGTTGAAATGGGAGTGCAATGGAATCCATGTGCCCTGTTACATTCTAGTACATGCTCA; TTGACTCATAGTGAGTCCGAAGTAAAGGAACACACATTTATATCCCAAACTAAGAGTTCTTATAAAAAAT; TAACATCAAAGAACAAAAGTAACACAATATTTAGTGATTCTTATATTAAGCATTCACCAAATATAGTGCA; AGACTTAAATAAAAGTAGAGAGCATACAAGTTCCTTTAATTGTAATTTCCAGAATGGGAATCAAGAACAT; CAGACATGTTCCATTTTCAGAGCAAGAAAACGGGCCTCTTTAGATATAAATAAAGAGAAGCCAGGAGCCT; CTCAGAATGAGGGGAAAACAAGTGATAAGAAAGTTGTTCAGACTTTTTCACAGAAAACAAAAAAGGCACC; TTTGAATTTCAATTCAGAAAAGATGAGCAGAAGTTTTCGATCTTGGAAACGTAGAAAGCATCTAAAGCGA; TCTAGGGACAGCAGCCCCCTGAAAGACTCTGGAGCGTGTAGAATCCATTTACAAGGACAGACTCTGTCTA; ATCCTAGTCTTTGTGAAGACCCGTTTACCTTAGATGAGAAGAAAACGGAATTTAGAAATTCAGGGCCATT; TGCTAAAAATGTATCTTTGAGTGGTAAGGAAAAAGATAATAAAACATCATTCCCATTACAAATAAAGCAA; AATTGTTCATGGAACATAACACTAACTAATGATAATTTTGTGGAGCATATTGTCACAGGATCTCAGAGTA; AAAATGTGACTTGTCAGGCCACTAGTGTGGTTAGTGAAAAGGGCAGAGGAGTAGCTGTTGAGGCAGAAAA; AATAAATGAAGTGCTGATACAAAATGGTTCAAAAAACCAGAATGTTTATATGAAACACCATGACATCCAT; CCAATTAACCAGTACCTGCGAAAGCAATCTCATGAACAGACAAGCACTATTACCAAACAGAAAAATATAA; TAGAGAGACAAATGCCCTGTGAAGCAGTCAGTAGTTACATAAATAGAGACTCAAATGTTACTATCAATTG; TGAAAGGATAAAGCTTAATACAGAGGAAAATAAACCAAGTCATTTTCAGGCATTAGGAGATGATATAAGC; AGAACTGTGATACCCAGTGAAGTACTTCCATCAGCTGGAGCATTTAGCAAATCAGAAGGCCAGCATGAGA; ATTTTCTAAATATTTCTAGACTACAAGAAAAAACAGGTACTTATACAACAAACAAAACTAAAAATAATCA; TGTTTCTGACTTAGGTTTAGTCCTCTGTGATTTTGAAGATAGTTTCTATCTGGATACTCAGTCAGAGAAA; ATAATACAACAGATGGCAACTGAAAATGCCAAACTAGGAGCAAAGGACACCAACCTGGCAGCAGGGATAA; TGCAGAAGAGCTTAGTCCAACAGAACTCAATGAACTCTTTTCAGAAGGAGTGTCACATTCCTTTTCCTGC; TGAACAGCACCCTCTAGGAGCGACTAAGATAGATCATTTGGACCTTAAGACTGTAGGTACTATGAAACAA; AGCAGTGATTCACATGGGGTTGATATCCTGACTCCAGAAAGCCCGATTTTCCATTCTCCAATACTATTGG; AGGAAAATGGTCTTTTTTTAAAAAAGAATGAAGTTTCTGTTACTGATTCACAATTAAATAGTTTTCTTCA; AGGTTATCAAACACAAGAAACTGTGAAACCAGTTATACTTCTGATTCCTCAAAAGAGAACTCCCACTGGT; GTAGAAGGAGAATGTCTTCCAGTTCCTGAAACAAGTTTGAATATGAGTGATAGTTTACTATTTGATAGCT; TCAGTGATGACTATCTAGTAAAAGAACAATTACCTGATATGCAAATGAAAGAACCCCTTCCTTCAGAAGT; AACATCAAACCATTTTAGTGATTCTCTGTGTCTACAAGAAGACCTAATTAAAAAATCAAATGTAAATGAG; AATCAAGATACCCACCAGCAGTTGACTTGTTCCAATGATGAATCTATTATATTTTCAGAAATGGATTCTG; TTCAGATGGTTGAAGCTTTGGACAATGTGGATATATTTCCTGTCCAAGAGAAGAATCATACTGTAGTATC; TCCTAGAGCATTAGAACTAAGTGATCCAGTACTTGATGAGCACCACCAAGGTGATCAAGATGGAGGAGAT; CAAGATGAAAGGGCTGAAAAATCAAAATTAACTGGGACCAGGCAAAATCATTCATTCATATGGTCAGGGG; CATCATTTGATCTAAGTCCAGGACTGCAAAGGATTTTAGATAAAGTATCCAGTCCTCTAGAAAATGAAAA; GCTAAAATCAATGACTATAAACTTTTCCAGTTTGAATAGAAAAAATACAGAGTTAAATGAAGAACAAGAA; GTTATTTCAAACTTGGAGACAAAACAAGTGCAGGGAATTTCATTTTCTTCTAATAATGAAGTAAAAAGCA; AGATTGAGATGCTAGAAAACAATGCCAATCATGATGAAACCTCATCCCTCTTACCTCGTAAAGAAAGTAA; TATAGTTGATGATAATGGTCTCATTCCTCCTACACCCATTCCAACATCTGCTTCTAAGCTGACATTTCCA; GGGATTCTTGAAACACCTGTAAACCCTTGGAAAACTAATAATGTTTTACAACCTGGTGAAAGTTATTTAT; TTGGCTCACCTTCAGATATTAAAAACCACGATTTAAGTCCAGGGAGTAGAAATGGGTTCAAAGACAACAG; CCCTATTAGTGACACAAGCTTTTCACTTCAGTTATCACAGGATGGATTACAGTTAACTCCAGCCTCAAGC; AGTTCAGAAAGTTTGTCCATAATTGATGTAGCAAGTGACCAAAATCTTTTCCAAACATTCATTAAGGAGT; GGCGGTGCAAAAAGCGATTTTCCATCTCACTGGCTTGTGAAAAGATTAGAAGTTTGACATCTTCTAAAAC; TGCTACTATTGGCAGTAGGTTTAAGCAAGCTAGCTCACCTCAGGAAATTCCTATTAGAGATGATGGATTT; CCCATTAAAGGTTGTGATGACACCTTGGTGGTTGGACTGGCAGTATGCTGGGGTGGAAGGGATGCCTATT; ATTTTTCACTGCAGAAGGAACAAAAGCATTCTGAAATTAGTGCCAGTTTGGTTCCACCTTCTTTAGATCC; AAGCCTGACTTTGAAAGACAGGATGTGGTACCTTCAATCTTGCTTGCGAAAGGAATCTGATAAAGAATGT; TCTGTTGTCATCTATGACTTCATCCAGAGCTATAAAATTCTTCTTCTTTCTTGTGGCATCTCCTTGGAGC; AAAGTTATGAAGATCCTAAGGTGGCATGCTGGTTACTAGATCCAGATTCTCAGGAGCCGACTCTTCATAG; CATAGTTACCAGTTTTCTTCCTCATGAGCTTCCACTCCTAGAAGGGATGGAGACCAGCCAAGGGATTCAA; AGCCTGGGGCTAAATGCTGGCAGTGAGCATTCTGGGCGATACAGAGCATCTGTGGAGTCCATTCTCATCT; TCAACTCTATGAATCAGCTCAACTCTTTGTTGCAGAAGGAAAACCTTCAAGATGTTTTCCGTAAGGTGGA; AATGCCCTCTCAGTACTGCTTGGCCTTGCTAGAACTAAATGGAATTGGCTTTAGTACTGCAGAATGTGAA; AGTCAGAAACATATAATGCAAGCCAAGCTGGATGCAATTGAGACCCAGGCCTATCAACTAGCTGGCCACA; GTTTTTCTTTCACCAGTTCAGATGACATCGCTGAGGTTTTATTTTTGGAATTGAAGTTGCCCCCAAATAG; AGAGATGAAAAACCAAGGCAGCAAGAAAACTCTGGGTTCTACCAGAAGAGGGATTGACAATGGACGCAAG; CTAAGGCTGGGAAGACAGTTCAGCACTAGTAAGGACGTTTTAAATAAATTAAAGGCATTACATCCTTTAC; CAGGCTTGATATTAGAATGGAGAAGAATCACTAATGCTATTACCAAAGTGGTCTTTCCCCTTCAGCGGGA; AAAGTGTCTTAATCCTTTTCTTGGAATGGAAAGAATCTATCCTGTATCACAGTCGCACACTGCTACAGGA; CGAATAACCTTTACAGAACCAAATATTCAGAATGTGCCAAGAGATTTTGAAATCAAAATGCCAACACTAG; TAGGAGAAAGCCCACCTTCTCAAGCTGTAGGCAAAGGCCTACTTCCCATGGGCAGAGGAAAATATAAGAA; GGGTTTCAGCGTGAATCCTAGATGCCAGGCACAGATGGAGGAGAGAGCTGCAGACAGAGGAATGCCATTT; TCAATTAGCATGCGACATGCCTTTGTGCCTTTCCCAGGTGGTTCAATACTGGCTGCTGACTACTCTCAGC; TTGAACTGAGGATCTTGGCTCATTTATCCCATGATCGTCGTCTCATTCAAGTGTTAAACACTGGAGCTGA; TGTTTTCAGGAGCATTGCAGCAGAGTGGAAGATGATTGAGCCAGAGTCTGTTGGGGATGATCTGAGGCAG; CAGGCAAAACAGATTTGCTATGGGATCATTTATGGAATGGGAGCTAAATCTTTGGGAGAGCAGATGGGCA; TTAAAGAAAATGATGCTGCATGCTATATTGACTCCTTCAAATCCAGATACACAGGGATTAATCAATTCAT; GACAGAGACAGTGAAGAATTGTAAAAGAGACGGATTTGTTCAGACCATTTTGGGAAGGCGTAGATATTTG; CCAGGAATCAAAGACAACAACCCTTATCGTAAAGCTCACGCTGAGCGTCAAGCTATCAACACAATAGTCC; AAGGATCAGCAGCTGATATTGTCAAAATAGCCACAGTTAACATTCAGAAGCAATTAGAGACCTTCCACTC; AACCTTCAAATCCCATGGTCATCGAGAGGGTATGCTCCAAAGTGACCAAACAGGATTGTCACGAAAGAGA; AAACTGCAAGGGATGTTCTGCCCAATCAGAGGAGGCTTCTTCATCCTTCAACTCCATGATGAACTCCTAT; ATGAAGTGGCAGAAGAAGATGTTGTTCAGGTAGCTCAGATTGTCAAGAATGAAATGGAAAGTGCTGTAAA; ACTGTCTGTGAAATTGAAAGTGAAAGTGAAAATAGGCGCCAGCTGGGGAGAGCTAAAGGACTTTGATGTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212566 representing NM_199420; Red=Cloning site Green=Tags(s); ; MNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPGLGRCLKAAAAGECKPTVPDYEIDKLL; LANWGLPKAVLEKYHSFGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKA; LFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLL; GMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNA; ELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWC; EKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGL; TFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVG; ESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLA; ASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLD; IFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKG; KVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLG; WHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFK; SARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCS; LTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEH; QTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKR; SRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQ; NCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIH; PINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDIS; RTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEK; IIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQ; SSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTG; VEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNE; NQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGD; QDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQE; VISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFP; GILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASS; SSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGF; PIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKEC; SVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQ; SLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECE; SQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRK; LRLGRQFSTSKDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATG; RITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADRGMPF; SISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQ; QAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYL; PGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTGLSRKR; KLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199420
• ORF Size: 7770 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_199420.2, NP_955452.2
• RefSeq Size: 8790 bp
• RefSeq ORF: 7773 bp
• Locus ID: 10721
• UniProt ID: O75417
• Cytogenetics: 3q13.33
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Summary: DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (By similarity). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (By similarity).[UniProtKB/Swiss-Prot Function]