IDN3 (NIPBL) (NM_015384) Human Tagged ORF Clone

SKU RG211931

NIPBL (tGFP-tagged) - Human Nipped-B homolog (Drosophila) (NIPBL), transcript variant B. Note: ORF is codon optimized

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: IDN3
• Synonyms: CDLS; CDLS1; IDN3; IDN3-B; Scc2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211931 ORF sequence, codon optimized.; Due to the complexity of NM_015384, the ORF clone is codon optimized for mammalian Expression. ; The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.; ; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAATGGAGATATGCCGCACGTGCCAATCACAACCCTTGCCGGGATTGCGTCACTCACGGACCTGCTTA; ATCAACTCCCACTGCCCAGTCCCCTGCCAGCCACTACCACAAAGTCACTCCTCTTTAACGCTCGCATTGC; CGAAGAAGTTAACTGCCTGCTCGCCTGCCGGGACGATAACTTGGTGTCACAGTTGGTACACAGCCTGAAT; CAGGTGTCCACCGACCACATTGAATTGAAAGACAATCTGGGAAGCGATGACCCCGAGGGCGACATCCCCG; TGCTGTTGCAGGCCGTTCTCGCCCGCTCACCTAATGTATTTAGGGAAAAGTCAATGCAGAACCGATACGT; ACAGAGTGGCATGATGATGAGCCAATACAAACTCAGTCAGAATAGTATGCATTCCTCACCCGCAAGTTCA; AACTACCAACAAACGACCATCTCTCATTCCCCTTCCAGTCGGTTTGTTCCACCCCAGACCTCCAGTGGGA; ATCGGTTTATGCCTCAGCAGAACTCTCCGGTCCCCAGCCCCTATGCCCCTCAGAGCCCTGCTGGGTATAT; GCCCTATTCACATCCGTCTAGCTATACCACCCATCCTCAGATGCAGCAGGCCAGTGTCTCATCTCCCATT; GTCGCCGGAGGACTGCGCAACATCCACGATAACAAAGTCAGCGGTCCACTGTCTGGGAACAGCGCCAACC; ATCACGCTGATAACCCCCGGCATGGGAGCAGCGAGGACTATCTGCATATGGTCCACCGCCTGAGCTCCGA; TGATGGAGACAGTAGTACAATGAGAAACGCTGCTAGCTTTCCACTTCGGTCACCACAACCAGTGTGTTCC; CCTGCGGGCTCTGAAGGGACTCCTAAAGGCAGCCGCCCTCCCCTGATCCTTCAGAGTCAATCCCTGCCAT; GCAGTAGCCCCAGAGATGTGCCGCCAGATATCCTGCTGGACTCCCCCGAGCGGAAGCAGAAAAAGCAAAA; AAAAATGAAGCTGGGGAAGGATGAGAAAGAACAGTCAGAGAAAGCGGCCATGTACGATATAATATCTTCT; CCATCAAAGGATTCTACCAAGCTCACACTGCGACTTAGTCGAGTTCGGTCCTCCGATATGGATCAGCAGG; AAGACATGATTAGCGGTGTCGAGAATTCCAACGTGAGCGAAAATGACATACCTTTTAATGTCCAGTACCC; CGGCCAAACCAGTAAAACCCCCATTACACCCCAGGACATAAATCGGCCCCTGAATGCAGCTCAATGCCTT; TCTCAGCAGGAACAGACGGCGTTTCTCCCCGCCAACCAGGTACCTGTTCTGCAACAGAACACTAGCGTTG; CCGCAAAGCAGCCCCAGACCTCCGTGGTTCAAAATCAGCAGCAGATTTCCCAGCAAGGACCAATCTACGA; TGAGGTGGAACTGGATGCATTGGCAGAGATAGAGAGAATTGAACGCGAGTCCGCCATTGAGAGGGAAAGA; TTTTCCAAGGAAGTTCAGGATAAAGATAAACCACTGAAAAAGCGCAAGCAGGATAGTTACCCCCAAGAGG; CGGGCGGAGCCACGGGAGGGAATAGGCCTGCCTCTCAAGAAACAGGATCTACAGGCAACGGCTCCCGCCC; AGCTCTTATGGTTTCAATCGACCTGCATCAGGCGGGGCGCGTTGATAGCCAAGCGTCTATCACGCAGGAC; AGCGACTCTATTAAGAAACCTGAGGAGATCAAACAGTGCAATGATGCCCCTGTCAGCGTCCTTCAGGAGG; ACATAGTAGGGAGTCTCAAAAGTACCCCTGAAAACCACCCTGAGACTCCTAAGAAAAAGTCCGACCCGGA; GTTGTCCAAATCAGAGATGAAACAAAGCGAAAGCCGCTTGGCAGAGAGCAAGCCTAACGAGAATCGATTG; GTCGAGACAAAATCTTCAGAGAATAAGCTGGAAACCAAAGTGGAAACTCAGACTGAGGAACTCAAGCAGA; ACGAGTCTCGAACTACGGAGTGTAAACAGAATGAAAGTACCATCGTGGAACCGAAACAGAATGAGAACAG; ACTGAGTGACACTAAACCCAACGACAATAAGCAGAACAATGGTCGGTCTGAGACTACTAAATCCCGACCT; GAGACACCAAAACAGAAAGGTGAATCCAGACCCGAGACACCCAAGCAGAAAAGCGATGGACATCCCGAAA; CACCTAAACAGAAGGGAGACGGGAGGCCTGAAACTCCGAAGCAAAAGGGTGAGAGTCGGCCCGAGACGCC; TAAGCAGAAGAATGAAGGCAGGCCTGAGACACCTAAGCACCGCCACGACAATCGGAGAGACAGCGGGAAG; CCTTCCACGGAAAAGAAGCCAGAGGTATCTAAGCACAAGCAAGACACCAAGTCAGATAGTCCAAGACTGA; AAAGTGAGCGCGCTGAGGCTCTGAAGCAGCGGCCCGACGGCCGCAGTGTGTCTGAATCATTGAGGAGAGA; TCATGACAACAAGCAGAAGTCCGATGACAGAGGGGAATCTGAACGGCATCGGGGCGATCAATCCCGGGTT; AGGCGGCCCGAAACTTTGCGGTCAAGTAGTAGAAATGAACACGGGATCAAAAGCGATTCTTCTAAAACCG; ATAAGCTGGAGAGAAAACATAGACACGAGAGTGGGGACTCTCGGGAGAGGCCCTCCTCTGGGGAGCAGAA; ATCACGCCCCGACTCACCCCGCGTGAAGCAGGGTGATAGTAACAAGTCACGGTCAGACAAACTCGGGTTC; AAAAGTCCCACCTCCAAAGATGATAAAAGGACCGAAGGAAACAAAAGTAAGGTGGACACAAATAAGGCCC; ATCCGGACAATAAGGCGGAATTTCCGAGCTACCTGCTGGGTGGTAGATCCGGCGCCCTGAAGAATTTCGT; TATACCTAAGATTAAGAGGGATAAAGACGGAAACGTGACACAGGAGACAAAAAAAATGGAAATGAAAGGG; GAGCCCAAGGACAAGGTTGAAAAAATCGGCCTTGTGGAGGACCTCAATAAAGGGGCGAAGCCTGTTGTGG; TGCTTCAGAAGTTGTCCCTGGATGATGTGCAAAAACTGATAAAAGATAGAGAAGATAAGTCCCGCAGCAG; CCTTAAGCCAATCAAGAACAAACCTAGCAAATCTAACAAAGGGTCTATCGACCAATCTGTGCTTAAGGAA; TTGCCTCCCGAACTCCTGGCCGAAATCGAGTCTACGATGCCGCTCTGCGAAAGGGTAAAAATGAATAAGC; GAAAACGGAGTACGGTGAACGAAAAACCTAAATACGCCGAAATTTCTAGCGATGAGGACAACGATTCAGA; CGAGGCATTTGAGAGCAGCCGCAAGCGCCACAAGAAGGACGATGATAAAGCATGGGAGTACGAAGAGCGA; GACAGGCGGAGCTCTGGGGATCACCGCAGATCAGGGCATAGTCACGAAGGCCGGCGCAGTTCCGGAGGGG; GCAGATATCGGAACAGGAGTCCCTCTGATTCCGACATGGAGGATTATTCTCCCCCACCCAGCCTTAGTGA; GGTTGCTCGCAAAATGAAAAAGAAGGAAAAACAAAAAAAGAGGAAGGCTTATGAGCCTAAGCTCACACCC; GAAGAAATGATGGATTCTTCAACCTTCAAACGCTTTACTGCCTCCATCGAAAACATCCTCGACAACCTGG; AAGACATGGACTTCACGGCCTTTGGCGACGATGACGAAATCCCCCAGGAACTGCTGCTGGGCAAGCACCA; ACTGAATGAGCTTGGGAGCGAATCTGCTAAAATCAAGGCCATGGGCATAATGGACAAGCTGAGTACAGAC; AAGACTGTCAAGGTCCTCAATATACTGGAAAAAAACATCCAGGACGGGTCCAAACTCTCAACGCTCCTTA; ATCATAACAATGACACAGAGGAGGAGGAGCGACTGTGGAGGGACTTGATCATGGAAAGGGTTACCAAGTC; TGCTGATGCCTGCCTTACCACTATCAATATAATGACCAGTCCCAATATGCCCAAAGCTGTTTACATAGAA; GACGTAATTGAGCGCGTGATCCAATACACCAAGTTCCATCTGCAGAACACACTGTACCCTCAATATGACC; CCGTGTACAGACTCGACCCCCATGGGGGGGGACTGCTGTCCTCTAAAGCAAAGCGCGCTAAATGTTCTAC; ACATAAACAGCGCGTCATCGTGATGCTGTACAATAAGGTCTGCGATATCGTCTCTAGCCTGTCTGAGCTC; CTCGAGATTCAGCTGCTGACAGACACTACGATTCTGCAGGTGTCCTCTATGGGGATTACCCCATTCTTCG; TCGAGAATGTCTCAGAACTGCAACTGTGTGCAATAAAACTTGTCACAGCCGTGTTCTCCAGATACGAAAA; ACACCGGCAGCTCATCCTTGAAGAAATCTTTACAAGCCTTGCCCGCCTGCCTACCTCTAAGAGAAGCCTC; CGCAATTTTCGACTGAACTCAAGCGACATGGATGGAGAGCCAATGTACATCCAGATGGTGACCGCACTGG; TACTTCAGCTGATCCAGTGTGTGGTCCACCTCCCAAGCAGTGAGAAGGACAGCAACGCAGAGGAAGACAG; CAACAAAAAAATAGACCAGGACGTGGTGATCACCAACTCTTATGAAACAGCTATGCGCACTGCACAGAAT; TTCCTTAGTATTTTCCTGAAGAAATGCGGCAGTAAGCAGGGCGAAGAGGACTATCGCCCCCTGTTTGAAA; ACTTCGTGCAAGACCTTCTGAGTACCGTGAACAAGCCTGAGTGGCCCGCTGCAGAATTGCTGCTCTCCCT; CCTGGGCCGCCTGCTTGTTCATCAATTTTCTAATAAATCCACCGAGATGGCCTTGCGCGTAGCCTCACTG; GACTACCTTGGAACCGTAGCCGCACGGCTGCGGAAAGACGCCGTTACAAGTAAGATGGATCAGGGAAGCA; TCGAACGAATTCTTAAGCAGGTTAGTGGAGGCGAGGACGAAATCCAGCAGCTCCAGAAAGCGCTGTTGGA; TTATCTGGATGAGAATACTGAGACTGACCCCTCACTCGTGTTCTCCCGGAAGTTCTACATTGCCCAGTGG; TTTCGCGATACCACCCTGGAGACGGAAAAGGCAATGAAGTCTCAGAAAGATGAGGAATCCAGCGAGGGCA; CACATCACGCTAAAGAGATCGAAACCACAGGTCAGATCATGCATCGAGCCGAAAACCGGAAGAAGTTCTT; GAGGAGTATCATTAAAACCACCCCTTCCCAGTTCTCAACACTTAAAATGAACTCCGACACCGTGGACTAC; GATGATGCCTGTCTTATCGTGAGATATTTGGCCTCTATGCGGCCATTCGCACAGAGCTTTGACATCTATC; TGACTCAGATCCTCAGAGTCCTCGGCGAGAATGCCATTGCCGTTAGAACCAAGGCCATGAAGTGTCTGTC; CGAAGTCGTCGCTGTTGATCCCTCTATTTTGGCTCGGCTCGACATGCAAAGAGGGGTCCACGGGAGGCTG; ATGGACAACAGTACATCAGTTAGGGAGGCAGCCGTGGAGTTGCTCGGGAGGTTCGTGCTCTGCCGACCTC; AGCTGGCCGAACAGTATTATGATATGTTGATTGAGCGCATTCTCGATACCGGAATAAGTGTGCGAAAACG; GGTCATCAAAATTTTGCGCGACATATGCATTGAGCAACCCACTTTCCCTAAGATAACAGAAATGTGTGTG; AAAATGATACGCAGAGTGAATGACGAAGAGGGGATTAAGAAGCTGGTCAACGAAACGTTTCAGAAGCTGT; GGTTTACCCCTACCCCACACAACGATAAGGAGGCCATGACGCGCAAAATCCTGAATATCACCGACGTAGT; GGCGGCATGCAGAGATACCGGGTACGACTGGTTTGAACAGTTGCTGCAGAACCTGCTGAAGTCCGAGGAG; GACTCATCTTACAAACCCGTAAAAAAGGCATGCACTCAGCTGGTGGATAACCTGGTGGAGCACATACTCA; AATATGAAGAGAGCCTCGCCGACAGCGATAACAAGGGCGTAAATTCCGGTAGACTGGTCGCCTGCATCAC; AACTCTTTTCTTGTTCAGCAAAATTAGACCCCAACTGATGGTCAAACACGCGATGACCATGCAGCCTTAC; CTGACTACTAAGTGCTCAACGCAAAATGACTTCATGGTAATATGTAACGTGGCAAAAATTCTGGAACTGG; TGGTGCCCCTGATGGAACATCCATCTGAAACTTTTCTCGCTACTATCGAGGAAGACTTGATGAAACTGAT; TATAAAGTATGGGATGACTGTGGTCCAACATTGCGTCAGCTGTCTTGGAGCTGTGGTTAATAAGGTTACG; CAGAACTTCAAGTTCGTTTGGGCGTGCTTCAACCGCTACTATGGGGCTATATCCAAACTGAAGAGCCAAC; ATCAAGAAGACCCGAATAATACCTCTCTGCTGACTAACAAACCCGCGTTGCTGCGGAGTTTGTTCACAGT; CGGAGCTTTGTGCAGACACTTCGACTTCGACCTGGAGGACTTCAAAGGGAACAGCAAAGTGAACATCAAG; GATAAGGTCCTGGAACTGCTTATGTACTTCACCAAACACTCCGATGAGGAGGTGCAAACCAAGGCCATCA; TCGGACTCGGCTTTGCGTTTATCCAGCATCCTTCTTTGATGTTCGAGCAGGAGGTGAAGAATCTTTACAA; TAACATCCTGTCAGACAAGAACTCCAGCGTGAACCTCAAGATACAGGTGTTGAAGAACCTCCAGACTTAC; CTTCAGGAGGAAGACACGAGAATGCAGCAAGCTGACAGGGATTGGAAGAAGGTGGCCAAGCAGGAGGACT; TGAAAGAGATGGGCGACGTCAGCAGCGGGATGTCCAGCTCAATTATGCAGCTGTACCTGAAACAGGTCCT; CGAAGCCTTTTTCCACACTCAGTCTAGCGTGCGACACTTCGCACTGAATGTAATTGCGCTGACTCTCAAC; CAGGGCCTGATCCACCCCGTGCAGTGCGTGCCTTATCTCATAGCAATGGGCACAGATCCAGAACCCGCCA; TGCGCAATAAGGCCGACCAGCAGCTGGTCGAAATCGATAAGAAGTATGCCGGCTTCATTCACATGAAGGC; CGTTGCAGGAATGAAAATGAGCTACCAAGTACAGCAGGCCATAAACACATGCCTTAAAGATCCCGTCAGA; GGCTTTCGCCAGGATGAAAGTAGCTCCGCCTTGTGCAGTCATCTGTATAGCATGATCCGCGGCAACAGGC; AGCATCGGAGGGCCTTCCTTATATCCCTGTTGAACCTCTTTGATGACACTGCCAAGACAGACGTAACAAT; GTTGCTTTATATAGCAGACAACCTGGCATGCTTCCCATACCAGACACAGGAGGAGCCACTCTTCATAATG; CACCACATTGACATCACATTGTCAGTTAGTGGTAGTAATCTGCTGCAGAGTTTTAAGGAGTCTATGGTTA; AGGACAAGAGGAAGGAGAGGAAAAGCTCCCCCAGTAAGGAGAACGAGAGCTCCGATTCCGAGGAGGAGGT; TAGTCGCCCTCGGAAGTCCCGCAAGAGAGTTGATTCAGACTCCGACTCTGACAGTGAGGACGACATAAAT; AGTGTAATGAAATGCCTCCCGGAAAATTCTGCACCCCTGATTGAGTTCGCCAACGTCAGTCAGGGCATTC; TGCTGCTTCTGATGCTCAAGCAACACTTGAAAAACCTCTGCGGTTTCTCTGACTCTAAGATTCAGAAATA; TTCACCCTCAGAAAGTGCAAAGGTCTACGATAAGGCCATAAACAGAAAGACTGGTGTGCACTTCCATCCG; AAGCAGACCCTCGACTTCCTGCGGAGCGACATGGCCAACTCCAAAATAACCGAGGAGGTTAAACGATCCA; TCGTGAAGCAATACCTGGACTTTAAGCTCCTTATGGAGCACCTTGATCCTGACGAAGAGGAGGAAGAGGG; GGAAGTCAGCGCGAGTACGAATGCCCGCAATAAGGCAATTACTAGTCTCTTGGGAGGAGGGTCCCCGAAA; AATAATACTGCCGCTGAAACAGAGGATGATGAAAGTGACGGAGAAGACAGGGGCGGAGGCACCAGCGGGG; TTAGAAGGCGGCGCTCTCAGAGGATCAGCCAGCGAATCACG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211931 representing NM_015384; Red=Cloning site Green=Tags(s); ; MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLN; QVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASS; NYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPI; VAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCS; PAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISS; PSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCL; SQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERER; FSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQD; SDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRL; VETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRP; ETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGK; PSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRV; RRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGF; KSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKG; EPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKE; LPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEER; DRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTP; EEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTD; KTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIE; DVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSEL; LEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSL; RNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQN; FLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASL; DYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQW; FRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDY; DDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRL; MDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCV; KMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEE; DSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPY; LTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVT; QNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIK; DKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTY; LQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLN; QGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVR; GFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIM; HHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDIN; SVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHP; KQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPK; NNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRIT; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015384
• ORF Size: 8091 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015384.4, NP_056199.2
• RefSeq Size: 10389 bp
• RefSeq ORF: 8094 bp
• Locus ID: 25836
• UniProt ID: Q6KC79
• Cytogenetics: 5p13.2
• Summary: This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and cognitive disability. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]