MKS1 (NM_017777) Human Tagged ORF Clone

SKU RG211884

MKS1 (tGFP-tagged) - Human Meckel syndrome, type 1 (MKS1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MKS1
• Synonyms: BBS13; JBTS28; MES; MKS; POC12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211884 representing NM_017777; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGAGACCGTCTGGAGCACTGACACCGGGGAGGCAGTGTATCGCTCCCGGGACCCCGTGCGCAACT; TGCGCCTCCGAGTCCACCTGCAAAGAATCACATCAAGCAACTTTCTTCATTATCAGCCTGCTGCCGAGCT; CGGGAAGGACCTCATAGACTTGGCCACTTTTAGGCCTCAGCCAACTGCCAGTGGACACCGCCCAGAGGAA; GACGAAGAGGAGGAGATTGTGATTGGGTGGCAGGAGAAGCTCTTTAGCCAGTTTGAAGTAGATCTGTACC; AAAATGAAACAGCCTGTCAGAGTCCTTTGGATTATCAGTACCGTCAGGAGATCCTGAAGCTGGAGAATTC; GGGTGGCAAGAAAAACCGACGAATCTTTACCTACACTGACTCTGATAGATACACCAATTTGGAGGAGCAC; TGTCAGAGAATGACCACTGCAGCCAGCGAGGTGCCTTCATTCTTGGTCGAGCGAATGGCAAATGTCAGGC; GTCGCCGGCAGGACAGGCGAGGGATGGAGGGCGGCATCCTCAAGTCACGCATCGTCACCTGGGAGCCCTC; AGAAGAGTTTGTCAGGAACAACCACGTCATTAACACCCCTCTTCAGACAATGCACATCATGGCAGACCTG; GGGCCCTATAAAAAGCTTGGCTATAAGAAGTATGAACATGTCCTGTGTACTCTGAAGGTGGATAGCAATG; GTGTGATCACAGTAAAGCCTGACTTCACGGGCCTCAAAGGACCCTACAGGATTGAGACGGAGGGGGAGAA; GCAGGAGCTGTGGAAATATACGATCGACAATGTTTCCCCCCACGCACAGCCGGAGGAGGAGGAGCGGGAA; CGGCGAGTGTTCAAGGATCTTTATGGCCGGCACAAGGAGTATCTCAGCAGCCTCGTAGGCACCGACTTTG; AGATGACTGTCCCAGGTGCCCTCCGGCTCTTTGTAAATGGAGAGGTCGTTTCAGCCCAAGGCTATGAGTA; TGACAATCTCTACGTCCACTTCTTTGTAGAATTGCCAACTGCTCACTGGTCAAGCCCAGCATTCCAGCAG; CTCTCAGGAGTAACACAGACCTGCACCACCAAGTCCCTGGCAATGGACAAGGTGGCTCACTTCTCCTACC; CATTCACGTTTGAAGCCTTCTTCCTCCATGAGGATGAATCTTCTGATGCACTCCCGGAGTGGCCTGTGCT; CTACTGTGAGGTCCTCTCGCTGGACTTCTGGCAGAGGTACCGTGTGGAAGGCTATGGGGCTGTGGTGCTG; CCTGCCACTCCAGGCTCACACACCCTGACAGTCTCCACGTGGAGACCTGTGGAGCTTGGCACGGTGGCTG; AGCTGAGGAGGTTTTTCATTGGCGGTTCTCTGGAACTGGAGGACCTCTCCTATGTACGGATACCAGGATC; CTTCAAGGGGGAACGCCTGAGCCGCTTTGGACTCCGCACAGAGACCACAGGCACTGTCACCTTCCGCTTG; CACTGTCTGCAGCAGTCCAGGGCCTTCATGGAATCGAGCTCCCTTCAGAAAAGGATGCGGAGTGTGTTGG; ACCGTCTGGAAGGGTTCAGCCAGCAGAGTTCCATTCACAATGTGCTAGAGGCCTTCCGTCGAGCCCGGCG; CCGCATGCAGGAGGCCCGGGAAAGCCTCCCGCAGGACCTAGTGAGCCCCTCTGGAACCCTGGTCTCC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211884 representing NM_017777; Red=Cloning site Green=Tags(s); ; MAETVWSTDTGEAVYRSRDPVRNLRLRVHLQRITSSNFLHYQPAAELGKDLIDLATFRPQPTASGHRPEE; DEEEEIVIGWQEKLFSQFEVDLYQNETACQSPLDYQYRQEILKLENSGGKKNRRIFTYTDSDRYTNLEEH; CQRMTTAASEVPSFLVERMANVRRRRQDRRGMEGGILKSRIVTWEPSEEFVRNNHVINTPLQTMHIMADL; GPYKKLGYKKYEHVLCTLKVDSNGVITVKPDFTGLKGPYRIETEGEKQELWKYTIDNVSPHAQPEEEERE; RRVFKDLYGRHKEYLSSLVGTDFEMTVPGALRLFVNGEVVSAQGYEYDNLYVHFFVELPTAHWSSPAFQQ; LSGVTQTCTTKSLAMDKVAHFSYPFTFEAFFLHEDESSDALPEWPVLYCEVLSLDFWQRYRVEGYGAVVL; PATPGSHTLTVSTWRPVELGTVAELRRFFIGGSLELEDLSYVRIPGSFKGERLSRFGLRTETTGTVTFRL; HCLQQSRAFMESSSLQKRMRSVLDRLEGFSQQSSIHNVLEAFRRARRRMQEARESLPQDLVSPSGTLVS; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017777
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017777.4
• RefSeq Size: 2402 bp
• RefSeq ORF: 1680 bp
• Locus ID: 54903
• UniProt ID: Q9NXB0
• Cytogenetics: 17q22
• Summary: The protein encoded by this gene localizes to the basal body and is required for formation of the primary cilium in ciliated epithelial cells. Mutations in this gene result in Meckel syndrome type 1 and in Bardet-Biedl syndrome type 13. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]